Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4837 and GFF4838 are separated by 51 nucleotides GFF4838 and GFF4839 are separated by 57 nucleotides
GFF4837 - 'GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)' transl_table=11, at 132,846 to 135,080
GFF4837
GFF4838 - 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-), at 135,132 to 136,427
GFF4838
GFF4839 - BarA sensory histidine kinase (= VarS = GacS), at 136,485 to 139,241
GFF4839
Position (kb) 135
136
137 Strain fitness (log2 ratio) -2
-1
0
1 at 134.152 kb on + strand, within GFF4837 at 134.190 kb on - strand, within GFF4837 at 134.309 kb on + strand, within GFF4837 at 134.310 kb on - strand, within GFF4837 at 134.312 kb on - strand, within GFF4837 at 134.312 kb on - strand, within GFF4837 at 134.312 kb on - strand, within GFF4837 at 134.315 kb on + strand, within GFF4837 at 134.399 kb on - strand, within GFF4837 at 134.479 kb on + strand, within GFF4837 at 134.537 kb on - strand, within GFF4837 at 134.664 kb on + strand, within GFF4837 at 134.665 kb on - strand, within GFF4837 at 134.800 kb on - strand, within GFF4837 at 134.814 kb on + strand, within GFF4837 at 134.815 kb on - strand, within GFF4837 at 134.871 kb on - strand at 134.871 kb on - strand at 135.051 kb on - strand at 135.054 kb on - strand at 135.056 kb on + strand at 135.056 kb on + strand at 135.057 kb on - strand at 135.057 kb on - strand at 135.119 kb on - strand at 135.133 kb on + strand at 135.133 kb on + strand at 135.134 kb on - strand at 135.275 kb on - strand, within GFF4838 at 135.300 kb on + strand, within GFF4838 at 135.632 kb on + strand, within GFF4838 at 135.642 kb on - strand, within GFF4838 at 135.669 kb on + strand, within GFF4838 at 135.705 kb on + strand, within GFF4838 at 135.718 kb on - strand, within GFF4838 at 136.265 kb on + strand, within GFF4838 at 136.308 kb on - strand at 136.495 kb on - strand at 136.629 kb on - strand at 136.662 kb on - strand at 136.670 kb on - strand at 136.698 kb on - strand at 136.868 kb on + strand, within GFF4839 at 136.869 kb on - strand, within GFF4839 at 137.005 kb on - strand, within GFF4839 at 137.005 kb on - strand, within GFF4839 at 137.045 kb on + strand, within GFF4839 at 137.118 kb on - strand, within GFF4839 at 137.118 kb on - strand, within GFF4839 at 137.253 kb on - strand, within GFF4839 at 137.253 kb on - strand, within GFF4839
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 134,152 + GFF4837 0.58 -0.1 134,190 - GFF4837 0.60 +0.2 134,309 + GFF4837 0.65 +0.6 134,310 - GFF4837 0.66 -0.9 134,312 - GFF4837 0.66 -1.4 134,312 - GFF4837 0.66 +0.1 134,312 - GFF4837 0.66 -0.6 134,315 + GFF4837 0.66 -2.2 134,399 - GFF4837 0.69 +0.7 134,479 + GFF4837 0.73 -0.1 134,537 - GFF4837 0.76 +0.0 134,664 + GFF4837 0.81 -0.9 134,665 - GFF4837 0.81 +0.0 134,800 - GFF4837 0.87 -0.0 134,814 + GFF4837 0.88 -0.6 134,815 - GFF4837 0.88 +0.1 134,871 - +0.7 134,871 - -0.4 135,051 - +0.4 135,054 - -0.9 135,056 + -0.1 135,056 + -0.3 135,057 - +0.4 135,057 - +1.1 135,119 - +0.1 135,133 + +0.1 135,133 + -0.2 135,134 - -0.1 135,275 - GFF4838 0.11 +0.1 135,300 + GFF4838 0.13 +0.3 135,632 + GFF4838 0.39 +0.5 135,642 - GFF4838 0.39 -1.7 135,669 + GFF4838 0.41 -1.2 135,705 + GFF4838 0.44 -0.5 135,718 - GFF4838 0.45 -0.2 136,265 + GFF4838 0.87 -1.0 136,308 - -0.5 136,495 - +0.6 136,629 - +0.6 136,662 - +0.5 136,670 - +1.0 136,698 - +1.0 136,868 + GFF4839 0.14 -0.1 136,869 - GFF4839 0.14 +1.4 137,005 - GFF4839 0.19 +0.3 137,005 - GFF4839 0.19 +0.7 137,045 + GFF4839 0.20 +0.1 137,118 - GFF4839 0.23 +1.2 137,118 - GFF4839 0.23 +0.4 137,253 - GFF4839 0.28 +0.2 137,253 - GFF4839 0.28 +1.5
Or see this region's nucleotide sequence