Strain Fitness in Pseudomonas simiae WCS417 around PS417_02460

Experiment: 1-ethyl-3-methylimidazolium chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_02450 and PS417_02455 are separated by 194 nucleotidesPS417_02455 and PS417_02460 overlap by 4 nucleotidesPS417_02460 and PS417_02465 are separated by 63 nucleotidesPS417_02465 and PS417_02470 overlap by 13 nucleotides PS417_02450: PS417_02450 - oligoribonuclease, at 561,268 to 561,810 _02450 PS417_02455: PS417_02455 - putative transporter, required for glycine utilization (from data), at 562,005 to 562,619 _02455 PS417_02460: PS417_02460 - epoxyqueuosine reductase, at 562,616 to 563,680 _02460 PS417_02465: PS417_02465 - carbohydrate kinase, at 563,744 to 564,604 _02465 PS417_02470: PS417_02470 - ATPase, at 564,592 to 565,062 _02470 Position (kb) 562 563 564Strain fitness (log2 ratio) -1 0 1 2 3at 562.020 kb on + strandat 562.020 kb on + strandat 562.447 kb on - strand, within PS417_02455at 562.480 kb on - strand, within PS417_02455at 562.480 kb on - strand, within PS417_02455at 562.482 kb on - strand, within PS417_02455at 562.482 kb on - strand, within PS417_02455at 562.494 kb on + strand, within PS417_02455at 562.600 kb on - strandat 562.618 kb on - strandat 562.828 kb on + strand, within PS417_02460at 562.828 kb on + strand, within PS417_02460at 562.829 kb on - strand, within PS417_02460at 562.829 kb on - strand, within PS417_02460at 562.829 kb on - strand, within PS417_02460at 563.041 kb on - strand, within PS417_02460at 563.167 kb on - strand, within PS417_02460at 563.190 kb on - strand, within PS417_02460at 563.379 kb on + strand, within PS417_02460at 563.380 kb on - strand, within PS417_02460at 563.402 kb on + strand, within PS417_02460at 563.434 kb on - strand, within PS417_02460at 563.434 kb on - strand, within PS417_02460at 563.517 kb on + strand, within PS417_02460at 563.518 kb on - strand, within PS417_02460at 563.518 kb on - strand, within PS417_02460at 563.518 kb on - strand, within PS417_02460at 563.713 kb on - strandat 563.775 kb on + strandat 563.776 kb on - strandat 563.776 kb on - strandat 563.776 kb on - strandat 563.776 kb on - strandat 563.776 kb on - strandat 563.776 kb on - strandat 563.862 kb on + strand, within PS417_02465at 563.862 kb on + strand, within PS417_02465at 563.862 kb on + strand, within PS417_02465at 563.862 kb on + strand, within PS417_02465at 563.863 kb on - strand, within PS417_02465at 564.275 kb on - strand, within PS417_02465at 564.465 kb on - strand, within PS417_02465at 564.555 kb on + strandat 564.602 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 200 mM
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562,020 + -0.4
562,020 + +1.5
562,447 - PS417_02455 0.72 +0.3
562,480 - PS417_02455 0.77 +0.1
562,480 - PS417_02455 0.77 +1.1
562,482 - PS417_02455 0.78 -0.3
562,482 - PS417_02455 0.78 -0.4
562,494 + PS417_02455 0.80 -0.3
562,600 - +1.5
562,618 - +0.7
562,828 + PS417_02460 0.20 +2.2
562,828 + PS417_02460 0.20 +2.0
562,829 - PS417_02460 0.20 +0.6
562,829 - PS417_02460 0.20 +0.5
562,829 - PS417_02460 0.20 +0.6
563,041 - PS417_02460 0.40 +0.3
563,167 - PS417_02460 0.52 +0.2
563,190 - PS417_02460 0.54 +0.2
563,379 + PS417_02460 0.72 +1.4
563,380 - PS417_02460 0.72 -1.3
563,402 + PS417_02460 0.74 +1.4
563,434 - PS417_02460 0.77 +2.9
563,434 - PS417_02460 0.77 -0.7
563,517 + PS417_02460 0.85 +1.8
563,518 - PS417_02460 0.85 +0.3
563,518 - PS417_02460 0.85 +0.3
563,518 - PS417_02460 0.85 +0.1
563,713 - -0.2
563,775 + -0.2
563,776 - +1.5
563,776 - -1.0
563,776 - -0.2
563,776 - -0.8
563,776 - +0.3
563,776 - -0.5
563,862 + PS417_02465 0.14 +0.5
563,862 + PS417_02465 0.14 +1.3
563,862 + PS417_02465 0.14 +1.3
563,862 + PS417_02465 0.14 +2.1
563,863 - PS417_02465 0.14 -1.0
564,275 - PS417_02465 0.62 -0.4
564,465 - PS417_02465 0.84 +1.0
564,555 + +2.0
564,602 + +1.5

Or see this region's nucleotide sequence