Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4737 and GFF4738 overlap by 4 nucleotides GFF4738 and GFF4739 are separated by 81 nucleotides GFF4739 and GFF4740 are separated by 24 nucleotides
GFF4737 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 44,779 to 45,537
GFF4737
GFF4738 - Type III secretion system protein, at 45,534 to 45,776
GFF4738
GFF4739 - PrgI protein, at 45,858 to 46,100
GFF4739
GFF4740 - MxiG protein; Pathogenicity 1 island effector protein, at 46,125 to 47,189
GFF4740
Position (kb) 45
46
47 Strain fitness (log2 ratio) -2
-1
0
1
2 at 45.000 kb on - strand, within GFF4737 at 45.078 kb on - strand, within GFF4737 at 45.079 kb on + strand, within GFF4737 at 45.080 kb on - strand, within GFF4737 at 45.144 kb on - strand, within GFF4737 at 45.147 kb on + strand, within GFF4737 at 45.147 kb on + strand, within GFF4737 at 45.148 kb on - strand, within GFF4737 at 45.223 kb on + strand, within GFF4737 at 45.314 kb on + strand, within GFF4737 at 45.329 kb on + strand, within GFF4737 at 45.370 kb on - strand, within GFF4737 at 45.408 kb on + strand, within GFF4737 at 45.442 kb on - strand, within GFF4737 at 45.518 kb on + strand at 45.520 kb on + strand at 45.521 kb on - strand at 45.544 kb on - strand at 45.611 kb on - strand, within GFF4738 at 45.686 kb on + strand, within GFF4738 at 45.825 kb on - strand at 45.840 kb on - strand at 45.851 kb on + strand at 45.891 kb on - strand, within GFF4739 at 45.907 kb on - strand, within GFF4739 at 46.021 kb on - strand, within GFF4739 at 46.032 kb on + strand, within GFF4739 at 46.033 kb on - strand, within GFF4739 at 46.116 kb on + strand at 46.145 kb on - strand at 46.244 kb on - strand, within GFF4740 at 46.244 kb on - strand, within GFF4740 at 46.267 kb on + strand, within GFF4740 at 46.267 kb on + strand, within GFF4740 at 46.268 kb on - strand, within GFF4740 at 46.272 kb on - strand, within GFF4740 at 46.302 kb on + strand, within GFF4740 at 46.346 kb on + strand, within GFF4740 at 46.364 kb on - strand, within GFF4740 at 46.588 kb on + strand, within GFF4740 at 46.588 kb on + strand, within GFF4740 at 46.610 kb on + strand, within GFF4740 at 46.695 kb on - strand, within GFF4740 at 46.695 kb on - strand, within GFF4740 at 46.721 kb on - strand, within GFF4740 at 47.007 kb on + strand, within GFF4740
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 45,000 - GFF4737 0.29 -0.0 45,078 - GFF4737 0.39 +0.3 45,079 + GFF4737 0.40 -0.2 45,080 - GFF4737 0.40 -0.5 45,144 - GFF4737 0.48 +0.7 45,147 + GFF4737 0.48 -0.1 45,147 + GFF4737 0.48 +0.4 45,148 - GFF4737 0.49 +0.6 45,223 + GFF4737 0.58 +0.7 45,314 + GFF4737 0.70 +0.0 45,329 + GFF4737 0.72 +0.0 45,370 - GFF4737 0.78 +2.1 45,408 + GFF4737 0.83 +0.1 45,442 - GFF4737 0.87 -0.9 45,518 + -0.3 45,520 + +0.9 45,521 - -0.0 45,544 - -0.3 45,611 - GFF4738 0.32 +0.5 45,686 + GFF4738 0.63 -0.0 45,825 - +0.6 45,840 - -0.1 45,851 + +0.3 45,891 - GFF4739 0.14 +0.3 45,907 - GFF4739 0.20 +0.9 46,021 - GFF4739 0.67 +0.6 46,032 + GFF4739 0.72 +0.3 46,033 - GFF4739 0.72 +0.2 46,116 + -0.6 46,145 - +0.2 46,244 - GFF4740 0.11 +0.1 46,244 - GFF4740 0.11 +0.3 46,267 + GFF4740 0.13 -1.5 46,267 + GFF4740 0.13 +0.0 46,268 - GFF4740 0.13 +0.1 46,272 - GFF4740 0.14 +0.2 46,302 + GFF4740 0.17 +0.3 46,346 + GFF4740 0.21 -0.3 46,364 - GFF4740 0.22 -1.2 46,588 + GFF4740 0.43 -1.1 46,588 + GFF4740 0.43 -0.6 46,610 + GFF4740 0.46 -0.7 46,695 - GFF4740 0.54 +0.1 46,695 - GFF4740 0.54 -0.2 46,721 - GFF4740 0.56 -0.1 47,007 + GFF4740 0.83 -2.7
Or see this region's nucleotide sequence