Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4685 and GFF4686 are separated by 89 nucleotides GFF4686 and GFF4687 are separated by 9 nucleotides GFF4687 and GFF4688 are separated by 59 nucleotides GFF4688 and GFF4689 are separated by 71 nucleotides
GFF4685 - Superoxide dismutase [Cu-Zn] precursor (EC 1.15.1.1), at 14,352 to 14,885
GFF4685
GFF4686 - Phage minor tail protein, at 14,975 to 15,670
GFF4686
GFF4687 - Phage tail assembly protein, at 15,680 to 16,417
GFF4687
GFF4688 - Phage tail assembly protein I, at 16,477 to 17,019
GFF4688
GFF4689 - Phage tail fiber protein, at 17,091 to 19,538
GFF4689
Position (kb) 15
16
17 Strain fitness (log2 ratio) -1
0
1
2 at 14.808 kb on + strand, within GFF4685 at 14.871 kb on + strand at 14.891 kb on - strand at 14.939 kb on - strand at 14.972 kb on + strand at 14.974 kb on + strand at 14.974 kb on + strand at 14.974 kb on + strand at 14.985 kb on + strand at 15.300 kb on - strand, within GFF4686 at 15.300 kb on - strand, within GFF4686 at 15.380 kb on - strand, within GFF4686 at 15.418 kb on + strand, within GFF4686 at 15.424 kb on + strand, within GFF4686 at 15.472 kb on + strand, within GFF4686 at 15.619 kb on + strand at 15.668 kb on + strand at 15.817 kb on + strand, within GFF4687 at 15.818 kb on - strand, within GFF4687 at 15.818 kb on - strand, within GFF4687 at 15.919 kb on + strand, within GFF4687 at 15.930 kb on + strand, within GFF4687 at 15.972 kb on + strand, within GFF4687 at 15.972 kb on + strand, within GFF4687 at 15.972 kb on + strand, within GFF4687 at 15.976 kb on + strand, within GFF4687 at 16.037 kb on + strand, within GFF4687 at 16.038 kb on - strand, within GFF4687 at 16.058 kb on + strand, within GFF4687 at 16.078 kb on + strand, within GFF4687 at 16.120 kb on + strand, within GFF4687 at 16.131 kb on - strand, within GFF4687 at 16.305 kb on - strand, within GFF4687 at 16.305 kb on - strand, within GFF4687 at 16.436 kb on + strand at 16.586 kb on + strand, within GFF4688 at 16.593 kb on + strand, within GFF4688 at 17.021 kb on - strand at 17.077 kb on - strand at 17.077 kb on - strand at 17.117 kb on + strand at 17.117 kb on + strand at 17.118 kb on - strand at 17.270 kb on + strand at 17.271 kb on - strand at 17.271 kb on - strand at 17.271 kb on - strand at 17.292 kb on + strand at 17.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 14,808 + GFF4685 0.85 -0.6 14,871 + -0.3 14,891 - -0.1 14,939 - +0.5 14,972 + +0.3 14,974 + -0.5 14,974 + -0.7 14,974 + -0.1 14,985 + +0.0 15,300 - GFF4686 0.47 +0.4 15,300 - GFF4686 0.47 -0.4 15,380 - GFF4686 0.58 +0.2 15,418 + GFF4686 0.64 -0.7 15,424 + GFF4686 0.65 -0.2 15,472 + GFF4686 0.71 +0.3 15,619 + +0.4 15,668 + +0.1 15,817 + GFF4687 0.19 +0.4 15,818 - GFF4687 0.19 -0.4 15,818 - GFF4687 0.19 +0.4 15,919 + GFF4687 0.32 -0.6 15,930 + GFF4687 0.34 -1.7 15,972 + GFF4687 0.40 -0.4 15,972 + GFF4687 0.40 -0.4 15,972 + GFF4687 0.40 -1.7 15,976 + GFF4687 0.40 +1.4 16,037 + GFF4687 0.48 +0.1 16,038 - GFF4687 0.49 -0.9 16,058 + GFF4687 0.51 -0.4 16,078 + GFF4687 0.54 -0.0 16,120 + GFF4687 0.60 +0.2 16,131 - GFF4687 0.61 -0.7 16,305 - GFF4687 0.85 +0.1 16,305 - GFF4687 0.85 -0.4 16,436 + -0.4 16,586 + GFF4688 0.20 +0.3 16,593 + GFF4688 0.21 +0.4 17,021 - +2.4 17,077 - +1.9 17,077 - -0.2 17,117 + +0.8 17,117 + -0.2 17,118 - -0.8 17,270 + +0.1 17,271 - +0.2 17,271 - -0.7 17,271 - +0.2 17,292 + +0.2 17,293 - +0.0
Or see this region's nucleotide sequence