Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4556 and GFF4557 are separated by 287 nucleotides GFF4557 and GFF4558 are separated by 22 nucleotides GFF4558 and GFF4559 are separated by 49 nucleotides
GFF4556 - Leucyl-tRNA synthetase (EC 6.1.1.4), at 339,339 to 341,921
GFF4556
GFF4557 - Hydrolase, UxaA family, at 342,209 to 342,499
GFF4557
GFF4558 - Altronate hydrolase (EC 4.2.1.7), at 342,522 to 343,688
GFF4558
GFF4559 - 2-keto-3-deoxygluconate permease (KDG permease), at 343,738 to 344,691
GFF4559
Position (kb) 342
343
344 Strain fitness (log2 ratio) -1
0
1 at 342.262 kb on + strand, within GFF4557 at 342.263 kb on - strand, within GFF4557 at 342.395 kb on - strand, within GFF4557 at 342.395 kb on - strand, within GFF4557 at 342.395 kb on - strand, within GFF4557 at 342.398 kb on + strand, within GFF4557 at 342.399 kb on - strand, within GFF4557 at 342.447 kb on - strand, within GFF4557 at 342.447 kb on - strand, within GFF4557 at 342.582 kb on + strand at 342.729 kb on - strand, within GFF4558 at 342.818 kb on + strand, within GFF4558 at 342.875 kb on + strand, within GFF4558 at 342.876 kb on - strand, within GFF4558 at 343.051 kb on + strand, within GFF4558 at 343.286 kb on + strand, within GFF4558 at 343.364 kb on + strand, within GFF4558 at 343.642 kb on + strand at 343.667 kb on + strand at 343.841 kb on + strand, within GFF4559 at 343.860 kb on + strand, within GFF4559 at 344.040 kb on - strand, within GFF4559 at 344.093 kb on + strand, within GFF4559 at 344.094 kb on - strand, within GFF4559 at 344.096 kb on - strand, within GFF4559 at 344.096 kb on - strand, within GFF4559 at 344.154 kb on - strand, within GFF4559 at 344.244 kb on + strand, within GFF4559 at 344.245 kb on - strand, within GFF4559 at 344.556 kb on + strand, within GFF4559 at 344.637 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 342,262 + GFF4557 0.18 +1.6 342,263 - GFF4557 0.19 +1.1 342,395 - GFF4557 0.64 +0.0 342,395 - GFF4557 0.64 +0.2 342,395 - GFF4557 0.64 -0.0 342,398 + GFF4557 0.65 -0.9 342,399 - GFF4557 0.65 +0.5 342,447 - GFF4557 0.82 +0.2 342,447 - GFF4557 0.82 -0.3 342,582 + -0.3 342,729 - GFF4558 0.18 -0.5 342,818 + GFF4558 0.25 +0.3 342,875 + GFF4558 0.30 -0.4 342,876 - GFF4558 0.30 -0.2 343,051 + GFF4558 0.45 -0.5 343,286 + GFF4558 0.65 +0.3 343,364 + GFF4558 0.72 -1.3 343,642 + +0.5 343,667 + -0.5 343,841 + GFF4559 0.11 +0.1 343,860 + GFF4559 0.13 +0.5 344,040 - GFF4559 0.32 +0.2 344,093 + GFF4559 0.37 -0.1 344,094 - GFF4559 0.37 -0.0 344,096 - GFF4559 0.38 +0.3 344,096 - GFF4559 0.38 +0.3 344,154 - GFF4559 0.44 +1.0 344,244 + GFF4559 0.53 -0.1 344,245 - GFF4559 0.53 -0.4 344,556 + GFF4559 0.86 -0.2 344,637 - +0.1
Or see this region's nucleotide sequence