Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4489 and GFF4490 overlap by 4 nucleotides GFF4490 and GFF4491 are separated by 48 nucleotides GFF4491 and GFF4492 are separated by 30 nucleotides GFF4492 and GFF4493 overlap by 4 nucleotides
GFF4489 - TonB-dependent receptor; Outer membrane receptor for ferric enterobactin and colicins B, D, at 271,772 to 274,027
GFF4489
GFF4490 - hypothetical protein, at 274,024 to 274,167
GFF4490
GFF4491 - Enterobactin esterase, at 274,216 to 275,430
GFF4491
GFF4492 - FIG005032: Putative cytoplasmic protein YbdZ in enterobactin biosynthesis operon, at 275,461 to 275,679
GFF4492
GFF4493 - Enterobactin synthetase component F, serine activating enzyme (EC 2.7.7.-), at 275,676 to 279,560
GFF4493
Position (kb) 274
275
276 Strain fitness (log2 ratio) -1
0
1
2 at 273.254 kb on - strand, within GFF4489 at 273.338 kb on - strand, within GFF4489 at 273.520 kb on + strand, within GFF4489 at 273.521 kb on - strand, within GFF4489 at 273.785 kb on + strand, within GFF4489 at 273.987 kb on - strand at 273.999 kb on - strand at 274.030 kb on + strand at 274.031 kb on - strand at 274.031 kb on - strand at 274.372 kb on + strand, within GFF4491 at 274.372 kb on + strand, within GFF4491 at 274.372 kb on + strand, within GFF4491 at 274.490 kb on - strand, within GFF4491 at 274.497 kb on + strand, within GFF4491 at 274.586 kb on + strand, within GFF4491 at 274.587 kb on - strand, within GFF4491 at 274.773 kb on - strand, within GFF4491 at 274.822 kb on - strand, within GFF4491 at 274.938 kb on + strand, within GFF4491 at 275.133 kb on - strand, within GFF4491 at 275.310 kb on + strand at 275.619 kb on + strand, within GFF4492 at 275.769 kb on + strand at 275.769 kb on + strand at 275.769 kb on + strand at 275.770 kb on - strand at 276.040 kb on + strand at 276.217 kb on - strand, within GFF4493 at 276.412 kb on + strand, within GFF4493 at 276.412 kb on + strand, within GFF4493
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 273,254 - GFF4489 0.66 -0.4 273,338 - GFF4489 0.69 +0.8 273,520 + GFF4489 0.77 -0.2 273,521 - GFF4489 0.78 +0.7 273,785 + GFF4489 0.89 -0.2 273,987 - +0.0 273,999 - +0.8 274,030 + -0.4 274,031 - +0.3 274,031 - -0.4 274,372 + GFF4491 0.13 +0.1 274,372 + GFF4491 0.13 +0.2 274,372 + GFF4491 0.13 +2.1 274,490 - GFF4491 0.23 +0.3 274,497 + GFF4491 0.23 +0.8 274,586 + GFF4491 0.30 -0.6 274,587 - GFF4491 0.31 +0.1 274,773 - GFF4491 0.46 +0.2 274,822 - GFF4491 0.50 +0.7 274,938 + GFF4491 0.59 -0.7 275,133 - GFF4491 0.75 +0.9 275,310 + -0.4 275,619 + GFF4492 0.72 +0.2 275,769 + -0.3 275,769 + +1.0 275,769 + -0.3 275,770 - -0.3 276,040 + +0.7 276,217 - GFF4493 0.14 +0.2 276,412 + GFF4493 0.19 -0.5 276,412 + GFF4493 0.19 -0.2
Or see this region's nucleotide sequence