Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF445 and GFF446 are separated by 174 nucleotides GFF446 and GFF447 are separated by 22 nucleotides
GFF445 - Xylulose kinase (EC 2.7.1.17), at 166,711 to 168,165
GFF445
GFF446 - Inner membrane protein YiaB, at 168,340 to 168,693
GFF446
GFF447 - Inner membrane protein YiaH, at 168,716 to 169,711
GFF447
Position (kb) 168
169 Strain fitness (log2 ratio) -1
0
1
2 at 167.384 kb on + strand, within GFF445 at 167.385 kb on - strand, within GFF445 at 167.385 kb on - strand, within GFF445 at 167.433 kb on - strand, within GFF445 at 167.570 kb on + strand, within GFF445 at 167.571 kb on - strand, within GFF445 at 167.704 kb on + strand, within GFF445 at 167.704 kb on + strand, within GFF445 at 167.822 kb on - strand, within GFF445 at 167.838 kb on + strand, within GFF445 at 167.912 kb on - strand, within GFF445 at 167.927 kb on + strand, within GFF445 at 167.928 kb on - strand, within GFF445 at 167.961 kb on + strand, within GFF445 at 168.022 kb on - strand at 168.044 kb on + strand at 168.044 kb on + strand at 168.207 kb on - strand at 168.241 kb on + strand at 168.390 kb on - strand, within GFF446 at 168.390 kb on - strand, within GFF446 at 168.409 kb on + strand, within GFF446 at 168.410 kb on - strand, within GFF446 at 168.458 kb on - strand, within GFF446 at 168.588 kb on - strand, within GFF446 at 168.648 kb on + strand, within GFF446 at 168.648 kb on + strand, within GFF446 at 168.648 kb on + strand, within GFF446 at 168.648 kb on + strand, within GFF446 at 168.648 kb on + strand, within GFF446 at 168.649 kb on - strand, within GFF446 at 168.650 kb on + strand, within GFF446 at 168.651 kb on - strand, within GFF446 at 168.651 kb on - strand, within GFF446 at 168.652 kb on + strand, within GFF446 at 168.653 kb on - strand, within GFF446 at 168.667 kb on + strand at 168.677 kb on - strand at 168.718 kb on - strand at 168.722 kb on - strand at 168.736 kb on + strand at 168.749 kb on + strand at 168.750 kb on - strand at 168.844 kb on + strand, within GFF447 at 168.848 kb on + strand, within GFF447 at 168.951 kb on - strand, within GFF447 at 169.002 kb on + strand, within GFF447 at 169.003 kb on - strand, within GFF447 at 169.034 kb on + strand, within GFF447 at 169.061 kb on - strand, within GFF447 at 169.151 kb on - strand, within GFF447 at 169.164 kb on + strand, within GFF447 at 169.167 kb on + strand, within GFF447 at 169.167 kb on + strand, within GFF447 at 169.168 kb on - strand, within GFF447 at 169.189 kb on - strand, within GFF447 at 169.189 kb on - strand, within GFF447 at 169.189 kb on - strand, within GFF447 at 169.189 kb on - strand, within GFF447 at 169.246 kb on + strand, within GFF447 at 169.255 kb on + strand, within GFF447 at 169.329 kb on + strand, within GFF447 at 169.421 kb on - strand, within GFF447 at 169.478 kb on - strand, within GFF447 at 169.639 kb on + strand at 169.639 kb on + strand at 169.639 kb on + strand at 169.639 kb on + strand at 169.640 kb on - strand at 169.640 kb on - strand at 169.640 kb on - strand at 169.642 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 167,384 + GFF445 0.46 +0.1 167,385 - GFF445 0.46 -0.0 167,385 - GFF445 0.46 +0.1 167,433 - GFF445 0.50 +0.3 167,570 + GFF445 0.59 -0.1 167,571 - GFF445 0.59 -0.5 167,704 + GFF445 0.68 +0.1 167,704 + GFF445 0.68 -0.4 167,822 - GFF445 0.76 +0.1 167,838 + GFF445 0.77 +0.5 167,912 - GFF445 0.83 +1.7 167,927 + GFF445 0.84 +0.4 167,928 - GFF445 0.84 +0.3 167,961 + GFF445 0.86 +0.2 168,022 - -0.1 168,044 + -0.1 168,044 + +0.2 168,207 - +0.1 168,241 + +0.5 168,390 - GFF446 0.14 -0.5 168,390 - GFF446 0.14 -0.7 168,409 + GFF446 0.19 -0.4 168,410 - GFF446 0.20 -0.0 168,458 - GFF446 0.33 +0.7 168,588 - GFF446 0.70 -0.2 168,648 + GFF446 0.87 +0.7 168,648 + GFF446 0.87 -0.2 168,648 + GFF446 0.87 -0.6 168,648 + GFF446 0.87 -0.1 168,648 + GFF446 0.87 -0.1 168,649 - GFF446 0.87 -0.4 168,650 + GFF446 0.88 -0.2 168,651 - GFF446 0.88 -0.3 168,651 - GFF446 0.88 -0.1 168,652 + GFF446 0.88 +0.1 168,653 - GFF446 0.88 -0.4 168,667 + -0.9 168,677 - -0.8 168,718 - +0.6 168,722 - -0.0 168,736 + +0.2 168,749 + -1.1 168,750 - -0.2 168,844 + GFF447 0.13 +0.0 168,848 + GFF447 0.13 -0.4 168,951 - GFF447 0.24 -0.2 169,002 + GFF447 0.29 -0.2 169,003 - GFF447 0.29 +1.1 169,034 + GFF447 0.32 -0.7 169,061 - GFF447 0.35 -0.0 169,151 - GFF447 0.44 +0.6 169,164 + GFF447 0.45 +0.3 169,167 + GFF447 0.45 +0.0 169,167 + GFF447 0.45 +0.3 169,168 - GFF447 0.45 +0.6 169,189 - GFF447 0.47 -0.4 169,189 - GFF447 0.47 -0.9 169,189 - GFF447 0.47 -0.3 169,189 - GFF447 0.47 -0.4 169,246 + GFF447 0.53 -0.0 169,255 + GFF447 0.54 +0.2 169,329 + GFF447 0.62 +0.2 169,421 - GFF447 0.71 +0.1 169,478 - GFF447 0.77 +2.4 169,639 + +0.7 169,639 + -0.2 169,639 + +0.0 169,639 + +0.4 169,640 - -0.4 169,640 - -1.0 169,640 - +0.0 169,642 + -0.6
Or see this region's nucleotide sequence