Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4254 and GFF4255 overlap by 4 nucleotides GFF4255 and GFF4256 overlap by 1 nucleotides GFF4256 and GFF4257 are separated by 205 nucleotides
GFF4254 - putative Cytochrome bd2, subunit I, at 38,978 to 40,381
GFF4254
GFF4255 - putative Cytochrome bd2, subunit II, at 40,378 to 41,388
GFF4255
GFF4256 - putative Cytochrome bd2, subunit II, at 41,388 to 41,519
GFF4256
GFF4257 - Transcriptional regulator, AraC family, at 41,725 to 42,729
GFF4257
Position (kb) 40
41
42 Strain fitness (log2 ratio) -2
-1
0
1 at 39.471 kb on + strand, within GFF4254 at 39.532 kb on + strand, within GFF4254 at 39.532 kb on + strand, within GFF4254 at 39.533 kb on - strand, within GFF4254 at 39.552 kb on + strand, within GFF4254 at 39.604 kb on + strand, within GFF4254 at 39.784 kb on + strand, within GFF4254 at 39.871 kb on + strand, within GFF4254 at 40.119 kb on + strand, within GFF4254 at 40.184 kb on - strand, within GFF4254 at 40.226 kb on + strand, within GFF4254 at 40.252 kb on + strand at 40.253 kb on - strand at 40.253 kb on - strand at 40.256 kb on + strand at 40.257 kb on - strand at 40.257 kb on - strand at 40.411 kb on - strand at 40.435 kb on + strand at 40.435 kb on + strand at 40.435 kb on + strand at 40.435 kb on + strand at 40.436 kb on - strand at 40.436 kb on - strand at 40.436 kb on - strand at 40.603 kb on + strand, within GFF4255 at 40.604 kb on - strand, within GFF4255 at 40.660 kb on - strand, within GFF4255 at 40.660 kb on - strand, within GFF4255 at 40.888 kb on + strand, within GFF4255 at 40.888 kb on + strand, within GFF4255 at 41.067 kb on - strand, within GFF4255 at 41.071 kb on + strand, within GFF4255 at 41.072 kb on - strand, within GFF4255 at 41.341 kb on + strand at 41.422 kb on + strand, within GFF4256 at 41.672 kb on - strand at 41.851 kb on - strand, within GFF4257 at 41.892 kb on - strand, within GFF4257 at 41.917 kb on - strand, within GFF4257 at 41.917 kb on - strand, within GFF4257 at 41.939 kb on + strand, within GFF4257 at 41.939 kb on + strand, within GFF4257 at 41.940 kb on - strand, within GFF4257 at 41.940 kb on - strand, within GFF4257 at 41.947 kb on + strand, within GFF4257 at 41.948 kb on - strand, within GFF4257 at 41.980 kb on + strand, within GFF4257 at 41.981 kb on - strand, within GFF4257 at 41.981 kb on - strand, within GFF4257 at 41.981 kb on - strand, within GFF4257 at 41.982 kb on + strand, within GFF4257 at 41.982 kb on + strand, within GFF4257 at 41.983 kb on - strand, within GFF4257 at 42.004 kb on - strand, within GFF4257 at 42.142 kb on + strand, within GFF4257 at 42.299 kb on + strand, within GFF4257 at 42.334 kb on + strand, within GFF4257
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 39,471 + GFF4254 0.35 +0.7 39,532 + GFF4254 0.39 -0.2 39,532 + GFF4254 0.39 +0.5 39,533 - GFF4254 0.40 +0.1 39,552 + GFF4254 0.41 +0.1 39,604 + GFF4254 0.45 +0.4 39,784 + GFF4254 0.57 -0.3 39,871 + GFF4254 0.64 -0.4 40,119 + GFF4254 0.81 -1.3 40,184 - GFF4254 0.86 +0.7 40,226 + GFF4254 0.89 -0.4 40,252 + -0.2 40,253 - -0.2 40,253 - +0.3 40,256 + -0.6 40,257 - +0.0 40,257 - -1.1 40,411 - +0.8 40,435 + +0.1 40,435 + -0.1 40,435 + +0.6 40,435 + +0.5 40,436 - +0.9 40,436 - -1.1 40,436 - +0.2 40,603 + GFF4255 0.22 +1.2 40,604 - GFF4255 0.22 +0.8 40,660 - GFF4255 0.28 -0.1 40,660 - GFF4255 0.28 +0.3 40,888 + GFF4255 0.50 -0.2 40,888 + GFF4255 0.50 -0.1 41,067 - GFF4255 0.68 +0.6 41,071 + GFF4255 0.69 -1.9 41,072 - GFF4255 0.69 +0.4 41,341 + +0.9 41,422 + GFF4256 0.26 -0.4 41,672 - +0.1 41,851 - GFF4257 0.13 -0.6 41,892 - GFF4257 0.17 +0.8 41,917 - GFF4257 0.19 +0.4 41,917 - GFF4257 0.19 -0.1 41,939 + GFF4257 0.21 -0.6 41,939 + GFF4257 0.21 +0.0 41,940 - GFF4257 0.21 +1.0 41,940 - GFF4257 0.21 -0.6 41,947 + GFF4257 0.22 +0.8 41,948 - GFF4257 0.22 +0.4 41,980 + GFF4257 0.25 +0.5 41,981 - GFF4257 0.25 -0.2 41,981 - GFF4257 0.25 -1.8 41,981 - GFF4257 0.25 -0.3 41,982 + GFF4257 0.26 -0.6 41,982 + GFF4257 0.26 -0.0 41,983 - GFF4257 0.26 +0.7 42,004 - GFF4257 0.28 -0.1 42,142 + GFF4257 0.41 -0.0 42,299 + GFF4257 0.57 +0.2 42,334 + GFF4257 0.61 -0.7
Or see this region's nucleotide sequence