Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3481 and GFF3482 are separated by 26 nucleotides GFF3482 and GFF3483 are separated by 95 nucleotides
GFF3481 - Nicotinamidase (EC 3.5.1.19), at 233,168 to 233,824
GFF3481
GFF3482 - L-asparaginase I, cytoplasmic (EC 3.5.1.1), at 233,851 to 234,867
GFF3482
GFF3483 - Signal peptide peptidase SppA (EC 3.4.21.-), at 234,963 to 236,819
GFF3483
Position (kb) 233
234
235 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 233.113 kb on + strand at 233.141 kb on + strand at 233.142 kb on - strand at 233.142 kb on - strand at 233.170 kb on - strand at 233.216 kb on - strand at 233.217 kb on + strand at 233.218 kb on - strand at 233.330 kb on + strand, within GFF3481 at 233.361 kb on + strand, within GFF3481 at 233.387 kb on - strand, within GFF3481 at 233.466 kb on + strand, within GFF3481 at 233.467 kb on - strand, within GFF3481 at 233.496 kb on + strand, within GFF3481 at 233.497 kb on - strand, within GFF3481 at 233.497 kb on - strand, within GFF3481 at 233.538 kb on - strand, within GFF3481 at 233.616 kb on + strand, within GFF3481 at 233.808 kb on - strand at 234.143 kb on + strand, within GFF3482 at 234.190 kb on + strand, within GFF3482 at 234.250 kb on - strand, within GFF3482 at 234.263 kb on - strand, within GFF3482 at 234.412 kb on - strand, within GFF3482 at 234.414 kb on + strand, within GFF3482 at 234.427 kb on + strand, within GFF3482 at 234.477 kb on + strand, within GFF3482 at 234.623 kb on + strand, within GFF3482 at 234.658 kb on - strand, within GFF3482 at 234.681 kb on + strand, within GFF3482 at 234.682 kb on - strand, within GFF3482 at 234.795 kb on + strand at 234.805 kb on - strand at 234.826 kb on - strand at 234.849 kb on - strand at 234.870 kb on + strand at 234.898 kb on - strand at 234.898 kb on - strand at 234.901 kb on - strand at 235.031 kb on + strand at 235.145 kb on + strand at 235.146 kb on - strand at 235.146 kb on - strand at 235.174 kb on + strand, within GFF3483 at 235.561 kb on + strand, within GFF3483 at 235.561 kb on + strand, within GFF3483 at 235.561 kb on + strand, within GFF3483 at 235.561 kb on + strand, within GFF3483 at 235.730 kb on + strand, within GFF3483 at 235.766 kb on + strand, within GFF3483
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 233,113 + -0.3 233,141 + +0.8 233,142 - +0.3 233,142 - -0.3 233,170 - -0.3 233,216 - -1.3 233,217 + -0.1 233,218 - +0.1 233,330 + GFF3481 0.25 -0.0 233,361 + GFF3481 0.29 +0.2 233,387 - GFF3481 0.33 +1.1 233,466 + GFF3481 0.45 -0.2 233,467 - GFF3481 0.46 +0.6 233,496 + GFF3481 0.50 +0.7 233,497 - GFF3481 0.50 +0.6 233,497 - GFF3481 0.50 -0.4 233,538 - GFF3481 0.56 +0.8 233,616 + GFF3481 0.68 +0.5 233,808 - +1.3 234,143 + GFF3482 0.29 -0.2 234,190 + GFF3482 0.33 -2.9 234,250 - GFF3482 0.39 -0.7 234,263 - GFF3482 0.41 -0.9 234,412 - GFF3482 0.55 -0.6 234,414 + GFF3482 0.55 -0.3 234,427 + GFF3482 0.57 -0.6 234,477 + GFF3482 0.62 -0.4 234,623 + GFF3482 0.76 +0.2 234,658 - GFF3482 0.79 -0.3 234,681 + GFF3482 0.82 -0.6 234,682 - GFF3482 0.82 -1.0 234,795 + -1.0 234,805 - -1.0 234,826 - -0.7 234,849 - -0.2 234,870 + -0.3 234,898 - -0.4 234,898 - -0.0 234,901 - -0.8 235,031 + -1.0 235,145 + -2.0 235,146 - +0.4 235,146 - -0.7 235,174 + GFF3483 0.11 -0.3 235,561 + GFF3483 0.32 +0.6 235,561 + GFF3483 0.32 -0.0 235,561 + GFF3483 0.32 -0.2 235,561 + GFF3483 0.32 +0.8 235,730 + GFF3483 0.41 +0.1 235,766 + GFF3483 0.43 -0.6
Or see this region's nucleotide sequence