Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3441 and GFF3442 overlap by 11 nucleotides GFF3442 and GFF3443 overlap by 7 nucleotides GFF3443 and GFF3444 overlap by 7 nucleotides GFF3444 and GFF3445 are separated by 81 nucleotides
GFF3441 - Nickel transport system permease protein nikB2 (TC 3.A.1.5.3), at 202,809 to 203,783
GFF3441
GFF3442 - Nickel transport system permease protein nikC2 (TC 3.A.1.5.3), at 203,773 to 204,585
GFF3442
GFF3443 - Nickel transport ATP-binding protein nikD2 (TC 3.A.1.5.3), at 204,579 to 205,376
GFF3443
GFF3444 - Nickel transport ATP-binding protein nikE2 (TC 3.A.1.5.3), at 205,370 to 205,960
GFF3444
GFF3445 - Putative inner membrane protein, at 206,042 to 207,175
GFF3445
Position (kb) 204
205
206 Strain fitness (log2 ratio) -1
0
1 at 203.689 kb on + strand at 203.701 kb on + strand at 203.701 kb on + strand at 203.702 kb on - strand at 203.705 kb on - strand at 203.705 kb on - strand at 203.821 kb on - strand at 203.951 kb on + strand, within GFF3442 at 204.293 kb on - strand, within GFF3442 at 204.491 kb on + strand, within GFF3442 at 204.503 kb on + strand, within GFF3442 at 204.655 kb on + strand at 204.788 kb on + strand, within GFF3443 at 204.818 kb on + strand, within GFF3443 at 204.818 kb on + strand, within GFF3443 at 204.819 kb on - strand, within GFF3443 at 204.819 kb on - strand, within GFF3443 at 204.849 kb on - strand, within GFF3443 at 204.965 kb on - strand, within GFF3443 at 204.968 kb on - strand, within GFF3443 at 205.077 kb on + strand, within GFF3443 at 205.077 kb on + strand, within GFF3443 at 205.078 kb on - strand, within GFF3443 at 205.080 kb on - strand, within GFF3443 at 205.245 kb on + strand, within GFF3443 at 205.246 kb on - strand, within GFF3443 at 205.246 kb on - strand, within GFF3443 at 205.248 kb on - strand, within GFF3443 at 205.334 kb on - strand at 205.366 kb on + strand at 205.366 kb on + strand at 205.856 kb on + strand, within GFF3444 at 205.856 kb on + strand, within GFF3444 at 205.857 kb on - strand, within GFF3444 at 205.857 kb on - strand, within GFF3444 at 205.857 kb on - strand, within GFF3444 at 205.903 kb on + strand at 205.920 kb on + strand at 206.086 kb on + strand at 206.092 kb on - strand at 206.256 kb on + strand, within GFF3445
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 203,689 + -0.3 203,701 + +0.1 203,701 + -0.1 203,702 - -0.2 203,705 - +0.5 203,705 - +0.1 203,821 - +0.1 203,951 + GFF3442 0.22 +0.2 204,293 - GFF3442 0.64 +0.9 204,491 + GFF3442 0.88 +0.4 204,503 + GFF3442 0.90 +0.2 204,655 + +0.9 204,788 + GFF3443 0.26 -1.0 204,818 + GFF3443 0.30 +0.0 204,818 + GFF3443 0.30 +0.4 204,819 - GFF3443 0.30 +0.3 204,819 - GFF3443 0.30 +1.4 204,849 - GFF3443 0.34 +0.3 204,965 - GFF3443 0.48 -0.2 204,968 - GFF3443 0.49 +0.8 205,077 + GFF3443 0.62 +0.9 205,077 + GFF3443 0.62 +0.5 205,078 - GFF3443 0.63 +0.2 205,080 - GFF3443 0.63 +0.4 205,245 + GFF3443 0.83 -0.3 205,246 - GFF3443 0.84 +0.8 205,246 - GFF3443 0.84 -0.1 205,248 - GFF3443 0.84 +0.1 205,334 - -0.9 205,366 + -0.0 205,366 + -0.2 205,856 + GFF3444 0.82 -1.3 205,856 + GFF3444 0.82 -0.3 205,857 - GFF3444 0.82 +0.0 205,857 - GFF3444 0.82 -0.8 205,857 - GFF3444 0.82 +0.6 205,903 + +0.5 205,920 + +0.2 206,086 + +0.2 206,092 - +0.3 206,256 + GFF3445 0.19 -0.7
Or see this region's nucleotide sequence