Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF325 and GFF326 are separated by 145 nucleotides GFF326 and GFF327 overlap by 4 nucleotides GFF327 and GFF328 are separated by 3 nucleotides
GFF325 - PTS system, gluconate-specific IIB component (EC 2.7.1.69), at 41,184 to 41,669
GFF325
GFF326 - PTS system, mannose-specific IIC component, at 41,815 to 42,564
GFF326
GFF327 - PTS system, mannose-specific IID component (EC 2.7.1.69), at 42,561 to 43,421
GFF327
GFF328 - L-seryl-tRNA(Sec) selenium transferase-related protein, at 43,425 to 44,534
GFF328
Position (kb) 42
43
44 Strain fitness (log2 ratio) -1
0
1 at 41.630 kb on + strand at 41.651 kb on + strand at 41.678 kb on + strand at 41.744 kb on + strand at 41.801 kb on + strand at 41.801 kb on + strand at 41.803 kb on + strand at 41.803 kb on + strand at 41.809 kb on - strand at 41.814 kb on + strand at 41.825 kb on + strand at 41.852 kb on - strand at 41.852 kb on - strand at 41.881 kb on + strand at 41.904 kb on - strand, within GFF326 at 41.949 kb on + strand, within GFF326 at 41.993 kb on - strand, within GFF326 at 42.054 kb on + strand, within GFF326 at 42.055 kb on - strand, within GFF326 at 42.245 kb on - strand, within GFF326 at 42.577 kb on + strand at 42.577 kb on + strand at 42.578 kb on - strand at 42.578 kb on - strand at 42.654 kb on - strand, within GFF327 at 42.688 kb on - strand, within GFF327 at 42.697 kb on + strand, within GFF327 at 42.791 kb on + strand, within GFF327 at 42.800 kb on + strand, within GFF327 at 42.842 kb on + strand, within GFF327 at 43.072 kb on - strand, within GFF327 at 43.094 kb on + strand, within GFF327 at 43.094 kb on + strand, within GFF327 at 43.094 kb on + strand, within GFF327 at 43.094 kb on + strand, within GFF327 at 43.095 kb on - strand, within GFF327 at 43.095 kb on - strand, within GFF327 at 43.095 kb on - strand, within GFF327 at 43.206 kb on + strand, within GFF327 at 43.207 kb on - strand, within GFF327 at 43.326 kb on + strand, within GFF327 at 43.332 kb on + strand, within GFF327 at 43.394 kb on - strand at 43.419 kb on + strand at 43.419 kb on + strand at 43.420 kb on - strand at 43.437 kb on - strand at 43.437 kb on - strand at 43.607 kb on + strand, within GFF328 at 43.622 kb on + strand, within GFF328 at 43.623 kb on - strand, within GFF328 at 43.623 kb on - strand, within GFF328 at 43.623 kb on - strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.627 kb on - strand, within GFF328 at 43.627 kb on - strand, within GFF328 at 43.627 kb on - strand, within GFF328 at 43.627 kb on - strand, within GFF328 at 43.742 kb on - strand, within GFF328 at 43.760 kb on + strand, within GFF328 at 44.045 kb on + strand, within GFF328 at 44.049 kb on + strand, within GFF328 at 44.050 kb on - strand, within GFF328 at 44.050 kb on - strand, within GFF328 at 44.070 kb on - strand, within GFF328 at 44.099 kb on + strand, within GFF328 at 44.099 kb on + strand, within GFF328 at 44.099 kb on + strand, within GFF328 at 44.190 kb on + strand, within GFF328 at 44.191 kb on - strand, within GFF328
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 41,630 + -0.2 41,651 + -0.0 41,678 + +0.2 41,744 + -0.1 41,801 + +0.5 41,801 + -0.2 41,803 + -0.1 41,803 + -0.6 41,809 - +0.8 41,814 + +0.4 41,825 + -0.4 41,852 - +0.0 41,852 - -0.5 41,881 + +0.5 41,904 - GFF326 0.12 +0.2 41,949 + GFF326 0.18 +0.0 41,993 - GFF326 0.24 -0.3 42,054 + GFF326 0.32 +0.3 42,055 - GFF326 0.32 +0.7 42,245 - GFF326 0.57 -0.8 42,577 + -0.3 42,577 + -0.3 42,578 - +0.3 42,578 - -0.5 42,654 - GFF327 0.11 -0.9 42,688 - GFF327 0.15 +0.0 42,697 + GFF327 0.16 +0.0 42,791 + GFF327 0.27 +0.2 42,800 + GFF327 0.28 -0.4 42,842 + GFF327 0.33 -0.1 43,072 - GFF327 0.59 -0.9 43,094 + GFF327 0.62 -0.2 43,094 + GFF327 0.62 +0.2 43,094 + GFF327 0.62 -0.4 43,094 + GFF327 0.62 -1.0 43,095 - GFF327 0.62 -0.1 43,095 - GFF327 0.62 -0.8 43,095 - GFF327 0.62 +1.5 43,206 + GFF327 0.75 +0.5 43,207 - GFF327 0.75 -0.7 43,326 + GFF327 0.89 -0.4 43,332 + GFF327 0.90 -0.4 43,394 - -0.1 43,419 + +0.2 43,419 + -0.3 43,420 - +0.0 43,437 - -1.1 43,437 - -0.9 43,607 + GFF328 0.16 +0.6 43,622 + GFF328 0.18 +0.2 43,623 - GFF328 0.18 -0.0 43,623 - GFF328 0.18 +1.1 43,623 - GFF328 0.18 -0.9 43,626 + GFF328 0.18 +0.6 43,626 + GFF328 0.18 -0.1 43,626 + GFF328 0.18 -0.5 43,626 + GFF328 0.18 -1.4 43,626 + GFF328 0.18 -0.5 43,626 + GFF328 0.18 -1.0 43,626 + GFF328 0.18 -0.6 43,626 + GFF328 0.18 +0.2 43,627 - GFF328 0.18 +0.2 43,627 - GFF328 0.18 -1.0 43,627 - GFF328 0.18 +0.0 43,627 - GFF328 0.18 -0.0 43,742 - GFF328 0.29 -0.1 43,760 + GFF328 0.30 +0.1 44,045 + GFF328 0.56 -0.0 44,049 + GFF328 0.56 +0.4 44,050 - GFF328 0.56 -0.0 44,050 - GFF328 0.56 +0.3 44,070 - GFF328 0.58 +0.5 44,099 + GFF328 0.61 -0.0 44,099 + GFF328 0.61 -0.2 44,099 + GFF328 0.61 +0.2 44,190 + GFF328 0.69 +0.6 44,191 - GFF328 0.69 -1.6
Or see this region's nucleotide sequence