Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3181 and GFF3182 are separated by 387 nucleotides GFF3182 and GFF3183 are separated by 11 nucleotides GFF3183 and GFF3184 are separated by 262 nucleotides
GFF3181 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 185,937 to 187,502
GFF3181
GFF3182 - Aerotaxis sensor receptor protein, at 187,890 to 189,410
GFF3182
GFF3183 - FIG01046167: hypothetical protein, at 189,422 to 189,577
GFF3183
GFF3184 - Putrescine aminotransferase (EC 2.6.1.82), at 189,840 to 191,219
GFF3184
Position (kb) 187
188
189
190 Strain fitness (log2 ratio) -1
0
1 at 186.927 kb on + strand, within GFF3181 at 186.927 kb on + strand, within GFF3181 at 186.948 kb on - strand, within GFF3181 at 187.025 kb on + strand, within GFF3181 at 187.097 kb on + strand, within GFF3181 at 187.221 kb on - strand, within GFF3181 at 187.230 kb on + strand, within GFF3181 at 187.370 kb on + strand at 187.371 kb on - strand at 187.661 kb on - strand at 187.663 kb on + strand at 187.794 kb on + strand at 187.875 kb on + strand at 187.875 kb on + strand at 187.885 kb on + strand at 188.066 kb on + strand, within GFF3182 at 188.103 kb on + strand, within GFF3182 at 188.337 kb on - strand, within GFF3182 at 188.337 kb on - strand, within GFF3182 at 188.341 kb on - strand, within GFF3182 at 188.369 kb on + strand, within GFF3182 at 188.576 kb on + strand, within GFF3182 at 188.577 kb on - strand, within GFF3182 at 188.577 kb on - strand, within GFF3182 at 188.721 kb on + strand, within GFF3182 at 188.810 kb on + strand, within GFF3182 at 188.912 kb on + strand, within GFF3182 at 188.913 kb on - strand, within GFF3182 at 189.023 kb on - strand, within GFF3182 at 189.025 kb on - strand, within GFF3182 at 189.025 kb on - strand, within GFF3182 at 189.077 kb on + strand, within GFF3182 at 189.077 kb on + strand, within GFF3182 at 189.077 kb on + strand, within GFF3182 at 189.077 kb on + strand, within GFF3182 at 189.077 kb on + strand, within GFF3182 at 189.078 kb on - strand, within GFF3182 at 189.078 kb on - strand, within GFF3182 at 189.078 kb on - strand, within GFF3182 at 189.080 kb on - strand, within GFF3182 at 189.199 kb on + strand, within GFF3182 at 189.199 kb on + strand, within GFF3182 at 189.206 kb on + strand, within GFF3182 at 189.207 kb on - strand, within GFF3182 at 189.323 kb on + strand at 189.323 kb on + strand at 189.325 kb on + strand at 189.325 kb on + strand at 189.325 kb on + strand at 189.326 kb on - strand at 189.326 kb on - strand at 189.345 kb on - strand at 189.359 kb on + strand at 189.360 kb on - strand at 189.360 kb on - strand at 189.360 kb on - strand at 189.381 kb on + strand at 189.538 kb on + strand, within GFF3183 at 189.800 kb on - strand at 189.817 kb on + strand at 189.818 kb on - strand at 189.850 kb on + strand at 189.966 kb on - strand at 189.977 kb on - strand at 190.202 kb on - strand, within GFF3184
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 186,927 + GFF3181 0.63 -1.6 186,927 + GFF3181 0.63 -0.0 186,948 - GFF3181 0.65 +0.1 187,025 + GFF3181 0.69 +0.7 187,097 + GFF3181 0.74 +1.5 187,221 - GFF3181 0.82 -1.1 187,230 + GFF3181 0.83 -0.2 187,370 + -0.4 187,371 - +0.3 187,661 - +0.0 187,663 + +0.3 187,794 + +0.3 187,875 + +0.1 187,875 + -1.0 187,885 + +0.4 188,066 + GFF3182 0.12 -0.0 188,103 + GFF3182 0.14 +0.0 188,337 - GFF3182 0.29 +0.2 188,337 - GFF3182 0.29 +0.5 188,341 - GFF3182 0.30 -1.2 188,369 + GFF3182 0.31 -0.0 188,576 + GFF3182 0.45 +0.0 188,577 - GFF3182 0.45 +1.5 188,577 - GFF3182 0.45 +0.2 188,721 + GFF3182 0.55 +0.5 188,810 + GFF3182 0.60 +0.2 188,912 + GFF3182 0.67 -0.5 188,913 - GFF3182 0.67 +0.4 189,023 - GFF3182 0.74 +0.8 189,025 - GFF3182 0.75 +0.5 189,025 - GFF3182 0.75 +1.0 189,077 + GFF3182 0.78 -0.2 189,077 + GFF3182 0.78 +0.3 189,077 + GFF3182 0.78 -0.0 189,077 + GFF3182 0.78 -0.1 189,077 + GFF3182 0.78 +1.1 189,078 - GFF3182 0.78 -0.1 189,078 - GFF3182 0.78 +0.1 189,078 - GFF3182 0.78 -0.0 189,080 - GFF3182 0.78 -0.1 189,199 + GFF3182 0.86 +0.1 189,199 + GFF3182 0.86 +0.4 189,206 + GFF3182 0.87 -0.6 189,207 - GFF3182 0.87 -1.0 189,323 + -0.3 189,323 + +0.1 189,325 + +1.2 189,325 + +1.5 189,325 + +0.1 189,326 - +0.2 189,326 - +0.8 189,345 - -0.3 189,359 + -0.0 189,360 - +0.7 189,360 - +0.8 189,360 - +0.1 189,381 + +0.8 189,538 + GFF3183 0.74 +0.5 189,800 - +0.7 189,817 + -0.2 189,818 - -0.1 189,850 + -0.0 189,966 - -0.1 189,977 - +0.2 190,202 - GFF3184 0.26 +1.2
Or see this region's nucleotide sequence