Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3157 and GFF3158 are separated by 19 nucleotides GFF3158 and GFF3159 are separated by 14 nucleotides GFF3159 and GFF3160 are separated by 170 nucleotides
GFF3157 - Putative arylsulfate sulfotransferase (EC 2.8.2.22), at 160,638 to 161,432
GFF3157
GFF3158 - Periplasmic thiol:disulfide interchange protein, DsbA-like, at 161,452 to 162,123
GFF3158
GFF3159 - Inner membrane thiol:disulfide oxidoreductase, DsbB-like, at 162,138 to 162,815
GFF3159
GFF3160 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12), at 162,986 to 163,639
GFF3160
Position (kb)
162
163 Strain fitness (log2 ratio)
-2
-1
0
1 at 161.197 kb on - strand, within GFF3157 at 161.208 kb on - strand, within GFF3157 at 161.214 kb on - strand, within GFF3157 at 161.217 kb on - strand, within GFF3157 at 161.274 kb on - strand, within GFF3157 at 161.477 kb on + strand at 161.586 kb on + strand, within GFF3158 at 162.047 kb on - strand, within GFF3158 at 162.047 kb on - strand, within GFF3158 at 162.051 kb on + strand, within GFF3158 at 162.052 kb on - strand, within GFF3158 at 162.052 kb on - strand, within GFF3158 at 162.056 kb on - strand at 162.285 kb on + strand, within GFF3159 at 162.318 kb on + strand, within GFF3159 at 162.347 kb on - strand, within GFF3159 at 162.370 kb on + strand, within GFF3159 at 162.441 kb on - strand, within GFF3159 at 162.449 kb on + strand, within GFF3159 at 162.458 kb on + strand, within GFF3159 at 162.478 kb on + strand, within GFF3159 at 162.482 kb on + strand, within GFF3159 at 162.483 kb on - strand, within GFF3159 at 162.550 kb on + strand, within GFF3159 at 162.647 kb on - strand, within GFF3159 at 162.690 kb on + strand, within GFF3159 at 162.734 kb on + strand, within GFF3159 at 162.734 kb on + strand, within GFF3159 at 162.734 kb on + strand, within GFF3159 at 162.735 kb on - strand, within GFF3159 at 162.735 kb on - strand, within GFF3159 at 162.735 kb on - strand, within GFF3159 at 162.735 kb on - strand, within GFF3159 at 162.735 kb on - strand, within GFF3159 at 162.826 kb on + strand at 162.917 kb on + strand at 162.917 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 161,197 - GFF3157 0.70 -0.2 161,208 - GFF3157 0.72 +0.2 161,214 - GFF3157 0.72 +0.4 161,217 - GFF3157 0.73 -0.4 161,274 - GFF3157 0.80 +0.2 161,477 + +0.1 161,586 + GFF3158 0.20 +0.6 162,047 - GFF3158 0.89 +1.0 162,047 - GFF3158 0.89 -0.1 162,051 + GFF3158 0.89 +0.1 162,052 - GFF3158 0.89 -0.1 162,052 - GFF3158 0.89 +0.7 162,056 - -0.1 162,285 + GFF3159 0.22 -0.9 162,318 + GFF3159 0.27 +0.2 162,347 - GFF3159 0.31 -0.7 162,370 + GFF3159 0.34 +0.6 162,441 - GFF3159 0.45 -2.7 162,449 + GFF3159 0.46 -0.8 162,458 + GFF3159 0.47 +0.7 162,478 + GFF3159 0.50 +1.1 162,482 + GFF3159 0.51 -0.3 162,483 - GFF3159 0.51 -0.0 162,550 + GFF3159 0.61 -0.4 162,647 - GFF3159 0.75 +0.2 162,690 + GFF3159 0.81 +0.2 162,734 + GFF3159 0.88 -0.3 162,734 + GFF3159 0.88 +0.5 162,734 + GFF3159 0.88 +0.1 162,735 - GFF3159 0.88 -1.2 162,735 - GFF3159 0.88 -0.8 162,735 - GFF3159 0.88 -0.3 162,735 - GFF3159 0.88 +0.2 162,735 - GFF3159 0.88 -0.2 162,826 + -0.3 162,917 + -0.4 162,917 + -0.3
Or see this region's nucleotide sequence