Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3116 and GFF3117 are separated by 112 nucleotides GFF3117 and GFF3118 are separated by 6 nucleotides GFF3118 and GFF3119 overlap by 8 nucleotides GFF3119 and GFF3120 are separated by 121 nucleotides
GFF3116 - FIG00553873: hypothetical protein, at 123,715 to 124,599
GFF3116
GFF3117 - Biopolymer transport protein ExbD/TolR, at 124,712 to 125,137
GFF3117
GFF3118 - MotA/TolQ/ExbB proton channel family protein, at 125,144 to 125,878
GFF3118
GFF3119 - hypothetical protein, at 125,871 to 126,008
GFF3119
GFF3120 - Cystathionine beta-lyase (EC 4.4.1.8), at 126,130 to 127,317
GFF3120
Position (kb) 125
126 Strain fitness (log2 ratio) -1
0
1 at 124.149 kb on - strand, within GFF3116 at 124.272 kb on + strand, within GFF3116 at 124.339 kb on + strand, within GFF3116 at 124.340 kb on - strand, within GFF3116 at 124.519 kb on + strand at 124.520 kb on - strand at 124.520 kb on - strand at 124.520 kb on - strand at 124.525 kb on + strand at 124.525 kb on + strand at 124.526 kb on - strand at 124.549 kb on + strand at 124.550 kb on - strand at 124.593 kb on - strand at 124.615 kb on - strand at 124.869 kb on - strand, within GFF3117 at 124.912 kb on + strand, within GFF3117 at 124.945 kb on - strand, within GFF3117 at 124.981 kb on + strand, within GFF3117 at 125.175 kb on - strand at 125.232 kb on - strand, within GFF3118 at 125.265 kb on + strand, within GFF3118 at 125.266 kb on - strand, within GFF3118 at 125.359 kb on + strand, within GFF3118 at 125.423 kb on - strand, within GFF3118 at 125.570 kb on - strand, within GFF3118 at 125.570 kb on - strand, within GFF3118 at 125.715 kb on - strand, within GFF3118 at 125.833 kb on - strand at 125.869 kb on + strand at 125.916 kb on + strand, within GFF3119 at 125.917 kb on - strand, within GFF3119 at 125.917 kb on - strand, within GFF3119 at 125.917 kb on - strand, within GFF3119 at 125.920 kb on - strand, within GFF3119 at 125.920 kb on - strand, within GFF3119 at 125.920 kb on - strand, within GFF3119 at 125.960 kb on + strand, within GFF3119 at 125.960 kb on + strand, within GFF3119 at 125.968 kb on + strand, within GFF3119 at 125.982 kb on + strand, within GFF3119 at 126.029 kb on - strand at 126.079 kb on + strand at 126.081 kb on + strand at 126.091 kb on - strand at 126.091 kb on - strand at 126.204 kb on + strand at 126.204 kb on + strand at 126.460 kb on + strand, within GFF3120 at 126.558 kb on + strand, within GFF3120 at 126.581 kb on + strand, within GFF3120 at 126.687 kb on + strand, within GFF3120 at 126.735 kb on + strand, within GFF3120 at 126.735 kb on + strand, within GFF3120 at 126.877 kb on + strand, within GFF3120 at 126.878 kb on - strand, within GFF3120 at 126.878 kb on - strand, within GFF3120 at 126.878 kb on - strand, within GFF3120
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 124,149 - GFF3116 0.49 +0.5 124,272 + GFF3116 0.63 -1.3 124,339 + GFF3116 0.71 -0.1 124,340 - GFF3116 0.71 +0.4 124,519 + +0.4 124,520 - +0.5 124,520 - -0.1 124,520 - +0.2 124,525 + -0.1 124,525 + -0.6 124,526 - +0.0 124,549 + +0.6 124,550 - -0.3 124,593 - -0.1 124,615 - +1.3 124,869 - GFF3117 0.37 +0.7 124,912 + GFF3117 0.47 +0.5 124,945 - GFF3117 0.55 -0.1 124,981 + GFF3117 0.63 +1.6 125,175 - -0.5 125,232 - GFF3118 0.12 +0.0 125,265 + GFF3118 0.16 +0.4 125,266 - GFF3118 0.17 +1.5 125,359 + GFF3118 0.29 +0.1 125,423 - GFF3118 0.38 +0.4 125,570 - GFF3118 0.58 +1.3 125,570 - GFF3118 0.58 +0.9 125,715 - GFF3118 0.78 -0.8 125,833 - +0.6 125,869 + +0.5 125,916 + GFF3119 0.33 -0.2 125,917 - GFF3119 0.33 +0.4 125,917 - GFF3119 0.33 -0.4 125,917 - GFF3119 0.33 +0.1 125,920 - GFF3119 0.36 -0.3 125,920 - GFF3119 0.36 -0.4 125,920 - GFF3119 0.36 -0.8 125,960 + GFF3119 0.64 +0.0 125,960 + GFF3119 0.64 -0.5 125,968 + GFF3119 0.70 -0.2 125,982 + GFF3119 0.80 -0.3 126,029 - -0.3 126,079 + +0.4 126,081 + +0.4 126,091 - -0.7 126,091 - +0.9 126,204 + +0.7 126,204 + -0.1 126,460 + GFF3120 0.28 -0.9 126,558 + GFF3120 0.36 +0.1 126,581 + GFF3120 0.38 +0.1 126,687 + GFF3120 0.47 -0.1 126,735 + GFF3120 0.51 -0.3 126,735 + GFF3120 0.51 +0.1 126,877 + GFF3120 0.63 +0.1 126,878 - GFF3120 0.63 +0.2 126,878 - GFF3120 0.63 +0.4 126,878 - GFF3120 0.63 +0.5
Or see this region's nucleotide sequence