Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3100 and GFF3101 are separated by 75 nucleotides GFF3101 and GFF3102 are separated by 27 nucleotides GFF3102 and GFF3103 overlap by 8 nucleotides GFF3103 and GFF3104 overlap by 8 nucleotides
GFF3100 - Possible ABC-transport protein, periplasmic-binding component, at 111,209 to 112,261
GFF3100
GFF3101 - [NiFe] hydrogenase metallocenter assembly protein HybG, at 112,337 to 112,585
GFF3101
GFF3102 - [NiFe] hydrogenase nickel incorporation protein HybF, at 112,613 to 112,954
GFF3102
GFF3103 - Hydrogenase-2 operon protein hybE, at 112,947 to 113,435
GFF3103
GFF3104 - Hydrogenase maturation protease (EC 3.4.24.-), at 113,428 to 113,922
GFF3104
Position (kb) 112
113 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 111.592 kb on - strand, within GFF3100 at 111.634 kb on - strand, within GFF3100 at 111.786 kb on + strand, within GFF3100 at 111.786 kb on + strand, within GFF3100 at 111.787 kb on - strand, within GFF3100 at 112.037 kb on + strand, within GFF3100 at 112.037 kb on + strand, within GFF3100 at 112.095 kb on - strand, within GFF3100 at 112.205 kb on + strand at 112.315 kb on + strand at 112.317 kb on + strand at 112.379 kb on + strand, within GFF3101 at 112.379 kb on + strand, within GFF3101 at 112.433 kb on + strand, within GFF3101 at 112.434 kb on - strand, within GFF3101 at 112.434 kb on - strand, within GFF3101 at 112.489 kb on + strand, within GFF3101 at 112.541 kb on - strand, within GFF3101 at 112.579 kb on + strand at 112.586 kb on - strand at 112.628 kb on - strand at 112.857 kb on - strand, within GFF3102 at 112.869 kb on - strand, within GFF3102 at 112.915 kb on + strand, within GFF3102 at 113.031 kb on + strand, within GFF3103 at 113.325 kb on - strand, within GFF3103
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 111,592 - GFF3100 0.36 -0.3 111,634 - GFF3100 0.40 +0.6 111,786 + GFF3100 0.55 -1.3 111,786 + GFF3100 0.55 +0.1 111,787 - GFF3100 0.55 +0.4 112,037 + GFF3100 0.79 -1.0 112,037 + GFF3100 0.79 -0.6 112,095 - GFF3100 0.84 +0.7 112,205 + -0.4 112,315 + -0.1 112,317 + -0.4 112,379 + GFF3101 0.17 -2.8 112,379 + GFF3101 0.17 -0.3 112,433 + GFF3101 0.39 -0.8 112,434 - GFF3101 0.39 -1.8 112,434 - GFF3101 0.39 -0.2 112,489 + GFF3101 0.61 +0.2 112,541 - GFF3101 0.82 -1.0 112,579 + +0.6 112,586 - -0.4 112,628 - -0.1 112,857 - GFF3102 0.71 +0.2 112,869 - GFF3102 0.75 +0.1 112,915 + GFF3102 0.88 +0.3 113,031 + GFF3103 0.17 +0.0 113,325 - GFF3103 0.77 +0.5
Or see this region's nucleotide sequence