Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3097

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3096 and GFF3097 are separated by 669 nucleotidesGFF3097 and GFF3098 are separated by 226 nucleotides GFF3096 - Putative methyl-accepting chemotaxis protein, at 105,754 to 106,812 GFF3096 GFF3097 - Glutathionylspermidine synthase (EC 6.3.1.8) / Glutathionylspermidine amidohydrolase (EC 3.5.1.78), at 107,482 to 109,338 GFF3097 GFF3098 - Uncharacterized GST-like protein yghU associated with glutathionylspermidine synthetase/amidase, at 109,565 to 110,431 GFF3098 Position (kb) 107 108 109 110Strain fitness (log2 ratio) -2 -1 0 1at 106.585 kb on - strand, within GFF3096at 107.204 kb on + strandat 107.206 kb on + strandat 107.385 kb on - strandat 107.590 kb on - strandat 107.603 kb on + strandat 107.718 kb on + strand, within GFF3097at 107.718 kb on + strand, within GFF3097at 107.721 kb on + strand, within GFF3097at 107.721 kb on + strand, within GFF3097at 107.908 kb on + strand, within GFF3097at 107.909 kb on - strand, within GFF3097at 107.909 kb on - strand, within GFF3097at 107.909 kb on - strand, within GFF3097at 107.990 kb on - strand, within GFF3097at 108.192 kb on + strand, within GFF3097at 108.193 kb on - strand, within GFF3097at 108.247 kb on + strand, within GFF3097at 108.274 kb on - strand, within GFF3097at 108.281 kb on + strand, within GFF3097at 108.282 kb on - strand, within GFF3097at 108.350 kb on + strand, within GFF3097at 108.356 kb on + strand, within GFF3097at 108.408 kb on + strand, within GFF3097at 108.409 kb on - strand, within GFF3097at 108.409 kb on - strand, within GFF3097at 108.486 kb on + strand, within GFF3097at 108.487 kb on - strand, within GFF3097at 108.587 kb on + strand, within GFF3097at 108.628 kb on + strand, within GFF3097at 108.629 kb on - strand, within GFF3097at 108.633 kb on - strand, within GFF3097at 108.753 kb on - strand, within GFF3097at 108.753 kb on - strand, within GFF3097at 108.822 kb on - strand, within GFF3097at 109.177 kb on - strandat 109.187 kb on + strandat 109.187 kb on + strandat 109.188 kb on - strandat 109.262 kb on + strandat 109.345 kb on - strandat 109.430 kb on + strandat 109.431 kb on - strandat 109.431 kb on - strandat 109.527 kb on - strandat 109.639 kb on + strandat 109.698 kb on + strand, within GFF3098at 109.741 kb on + strand, within GFF3098at 109.742 kb on - strand, within GFF3098at 110.057 kb on + strand, within GFF3098at 110.092 kb on + strand, within GFF3098at 110.145 kb on + strand, within GFF3098at 110.177 kb on + strand, within GFF3098at 110.182 kb on + strand, within GFF3098at 110.183 kb on - strand, within GFF3098at 110.183 kb on - strand, within GFF3098at 110.237 kb on + strand, within GFF3098at 110.237 kb on + strand, within GFF3098at 110.271 kb on - strand, within GFF3098at 110.280 kb on + strand, within GFF3098at 110.281 kb on - strand, within GFF3098

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
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106,585 - GFF3096 0.78 +0.2
107,204 + +0.2
107,206 + +0.1
107,385 - +0.5
107,590 - +0.9
107,603 + +0.1
107,718 + GFF3097 0.13 +0.1
107,718 + GFF3097 0.13 -0.8
107,721 + GFF3097 0.13 -0.4
107,721 + GFF3097 0.13 +0.4
107,908 + GFF3097 0.23 -0.1
107,909 - GFF3097 0.23 +0.3
107,909 - GFF3097 0.23 +0.1
107,909 - GFF3097 0.23 -0.3
107,990 - GFF3097 0.27 +0.1
108,192 + GFF3097 0.38 -0.1
108,193 - GFF3097 0.38 +0.2
108,247 + GFF3097 0.41 +0.0
108,274 - GFF3097 0.43 +0.0
108,281 + GFF3097 0.43 -2.7
108,282 - GFF3097 0.43 +0.3
108,350 + GFF3097 0.47 -0.5
108,356 + GFF3097 0.47 -0.0
108,408 + GFF3097 0.50 +0.2
108,409 - GFF3097 0.50 +0.3
108,409 - GFF3097 0.50 -0.5
108,486 + GFF3097 0.54 -0.2
108,487 - GFF3097 0.54 +1.0
108,587 + GFF3097 0.60 +0.8
108,628 + GFF3097 0.62 +0.4
108,629 - GFF3097 0.62 +0.9
108,633 - GFF3097 0.62 +0.7
108,753 - GFF3097 0.68 +0.4
108,753 - GFF3097 0.68 +0.8
108,822 - GFF3097 0.72 -0.3
109,177 - +0.1
109,187 + -0.9
109,187 + +0.7
109,188 - -0.9
109,262 + +0.2
109,345 - -0.1
109,430 + +0.0
109,431 - +0.4
109,431 - +0.5
109,527 - -1.3
109,639 + -0.2
109,698 + GFF3098 0.15 +1.1
109,741 + GFF3098 0.20 +0.2
109,742 - GFF3098 0.20 +0.2
110,057 + GFF3098 0.57 +0.1
110,092 + GFF3098 0.61 -0.1
110,145 + GFF3098 0.67 +0.1
110,177 + GFF3098 0.71 +0.1
110,182 + GFF3098 0.71 -0.1
110,183 - GFF3098 0.71 -0.0
110,183 - GFF3098 0.71 +1.1
110,237 + GFF3098 0.78 +0.4
110,237 + GFF3098 0.78 -0.5
110,271 - GFF3098 0.81 -0.0
110,280 + GFF3098 0.82 -0.4
110,281 - GFF3098 0.83 -0.0

Or see this region's nucleotide sequence