Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3096 and GFF3097 are separated by 669 nucleotides GFF3097 and GFF3098 are separated by 226 nucleotides
GFF3096 - Putative methyl-accepting chemotaxis protein, at 105,754 to 106,812
GFF3096
GFF3097 - Glutathionylspermidine synthase (EC 6.3.1.8) / Glutathionylspermidine amidohydrolase (EC 3.5.1.78), at 107,482 to 109,338
GFF3097
GFF3098 - Uncharacterized GST-like protein yghU associated with glutathionylspermidine synthetase/amidase, at 109,565 to 110,431
GFF3098
Position (kb)
107
108
109
110 Strain fitness (log2 ratio)
-2
-1
0
1 at 106.585 kb on - strand, within GFF3096 at 107.204 kb on + strand at 107.206 kb on + strand at 107.385 kb on - strand at 107.590 kb on - strand at 107.603 kb on + strand at 107.718 kb on + strand, within GFF3097 at 107.718 kb on + strand, within GFF3097 at 107.721 kb on + strand, within GFF3097 at 107.721 kb on + strand, within GFF3097 at 107.908 kb on + strand, within GFF3097 at 107.909 kb on - strand, within GFF3097 at 107.909 kb on - strand, within GFF3097 at 107.909 kb on - strand, within GFF3097 at 107.990 kb on - strand, within GFF3097 at 108.192 kb on + strand, within GFF3097 at 108.193 kb on - strand, within GFF3097 at 108.247 kb on + strand, within GFF3097 at 108.274 kb on - strand, within GFF3097 at 108.281 kb on + strand, within GFF3097 at 108.282 kb on - strand, within GFF3097 at 108.350 kb on + strand, within GFF3097 at 108.356 kb on + strand, within GFF3097 at 108.408 kb on + strand, within GFF3097 at 108.409 kb on - strand, within GFF3097 at 108.409 kb on - strand, within GFF3097 at 108.486 kb on + strand, within GFF3097 at 108.487 kb on - strand, within GFF3097 at 108.587 kb on + strand, within GFF3097 at 108.628 kb on + strand, within GFF3097 at 108.629 kb on - strand, within GFF3097 at 108.633 kb on - strand, within GFF3097 at 108.753 kb on - strand, within GFF3097 at 108.753 kb on - strand, within GFF3097 at 108.822 kb on - strand, within GFF3097 at 109.177 kb on - strand at 109.187 kb on + strand at 109.187 kb on + strand at 109.188 kb on - strand at 109.262 kb on + strand at 109.345 kb on - strand at 109.430 kb on + strand at 109.431 kb on - strand at 109.431 kb on - strand at 109.527 kb on - strand at 109.639 kb on + strand at 109.698 kb on + strand, within GFF3098 at 109.741 kb on + strand, within GFF3098 at 109.742 kb on - strand, within GFF3098 at 110.057 kb on + strand, within GFF3098 at 110.092 kb on + strand, within GFF3098 at 110.145 kb on + strand, within GFF3098 at 110.177 kb on + strand, within GFF3098 at 110.182 kb on + strand, within GFF3098 at 110.183 kb on - strand, within GFF3098 at 110.183 kb on - strand, within GFF3098 at 110.237 kb on + strand, within GFF3098 at 110.237 kb on + strand, within GFF3098 at 110.271 kb on - strand, within GFF3098 at 110.280 kb on + strand, within GFF3098 at 110.281 kb on - strand, within GFF3098
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 106,585 - GFF3096 0.78 +0.2 107,204 + +0.2 107,206 + +0.1 107,385 - +0.5 107,590 - +0.9 107,603 + +0.1 107,718 + GFF3097 0.13 +0.1 107,718 + GFF3097 0.13 -0.8 107,721 + GFF3097 0.13 -0.4 107,721 + GFF3097 0.13 +0.4 107,908 + GFF3097 0.23 -0.1 107,909 - GFF3097 0.23 +0.3 107,909 - GFF3097 0.23 +0.1 107,909 - GFF3097 0.23 -0.3 107,990 - GFF3097 0.27 +0.1 108,192 + GFF3097 0.38 -0.1 108,193 - GFF3097 0.38 +0.2 108,247 + GFF3097 0.41 +0.0 108,274 - GFF3097 0.43 +0.0 108,281 + GFF3097 0.43 -2.7 108,282 - GFF3097 0.43 +0.3 108,350 + GFF3097 0.47 -0.5 108,356 + GFF3097 0.47 -0.0 108,408 + GFF3097 0.50 +0.2 108,409 - GFF3097 0.50 +0.3 108,409 - GFF3097 0.50 -0.5 108,486 + GFF3097 0.54 -0.2 108,487 - GFF3097 0.54 +1.0 108,587 + GFF3097 0.60 +0.8 108,628 + GFF3097 0.62 +0.4 108,629 - GFF3097 0.62 +0.9 108,633 - GFF3097 0.62 +0.7 108,753 - GFF3097 0.68 +0.4 108,753 - GFF3097 0.68 +0.8 108,822 - GFF3097 0.72 -0.3 109,177 - +0.1 109,187 + -0.9 109,187 + +0.7 109,188 - -0.9 109,262 + +0.2 109,345 - -0.1 109,430 + +0.0 109,431 - +0.4 109,431 - +0.5 109,527 - -1.3 109,639 + -0.2 109,698 + GFF3098 0.15 +1.1 109,741 + GFF3098 0.20 +0.2 109,742 - GFF3098 0.20 +0.2 110,057 + GFF3098 0.57 +0.1 110,092 + GFF3098 0.61 -0.1 110,145 + GFF3098 0.67 +0.1 110,177 + GFF3098 0.71 +0.1 110,182 + GFF3098 0.71 -0.1 110,183 - GFF3098 0.71 -0.0 110,183 - GFF3098 0.71 +1.1 110,237 + GFF3098 0.78 +0.4 110,237 + GFF3098 0.78 -0.5 110,271 - GFF3098 0.81 -0.0 110,280 + GFF3098 0.82 -0.4 110,281 - GFF3098 0.83 -0.0
Or see this region's nucleotide sequence