Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3036

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3035 and GFF3036 are separated by 810 nucleotidesGFF3036 and GFF3037 overlap by 19 nucleotidesGFF3037 and GFF3038 are separated by 216 nucleotides GFF3035 - D-mannonate oxidoreductase (EC 1.1.1.57), at 49,500 to 50,972 GFF3035 GFF3036 - Muconolactone isomerase (EC 5.3.3.4),putative, at 51,783 to 51,917 GFF3036 GFF3037 - hypothetical protein, at 51,899 to 52,015 GFF3037 GFF3038 - Hexuronate utilization operon transcriptional repressor ExuR, at 52,232 to 52,981 GFF3038 Position (kb) 51 52Strain fitness (log2 ratio) -1 0 1 2at 50.883 kb on - strandat 50.887 kb on - strandat 50.887 kb on - strandat 51.651 kb on + strandat 51.651 kb on + strandat 51.652 kb on - strandat 51.652 kb on - strandat 51.652 kb on - strandat 51.749 kb on + strandat 51.754 kb on - strandat 51.774 kb on - strandat 51.781 kb on - strandat 51.940 kb on + strand, within GFF3037at 51.955 kb on - strand, within GFF3037at 52.046 kb on + strandat 52.047 kb on - strandat 52.100 kb on + strandat 52.101 kb on - strandat 52.113 kb on + strandat 52.114 kb on - strandat 52.381 kb on + strand, within GFF3038at 52.382 kb on - strand, within GFF3038at 52.429 kb on - strand, within GFF3038at 52.607 kb on - strand, within GFF3038at 52.609 kb on + strand, within GFF3038at 52.609 kb on + strand, within GFF3038at 52.610 kb on - strand, within GFF3038at 52.624 kb on + strand, within GFF3038at 52.624 kb on + strand, within GFF3038at 52.670 kb on - strand, within GFF3038at 52.722 kb on + strand, within GFF3038at 52.771 kb on - strand, within GFF3038at 52.820 kb on - strand, within GFF3038

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
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50,883 - +0.0
50,887 - -0.5
50,887 - -0.6
51,651 + -0.4
51,651 + +0.3
51,652 - -0.6
51,652 - +0.0
51,652 - +1.2
51,749 + +0.6
51,754 - +0.6
51,774 - -0.5
51,781 - -0.2
51,940 + GFF3037 0.35 +0.2
51,955 - GFF3037 0.48 +0.4
52,046 + +0.5
52,047 - +0.1
52,100 + -0.3
52,101 - +0.3
52,113 + -0.6
52,114 - -0.8
52,381 + GFF3038 0.20 +0.2
52,382 - GFF3038 0.20 +2.2
52,429 - GFF3038 0.26 +1.0
52,607 - GFF3038 0.50 -0.5
52,609 + GFF3038 0.50 +0.1
52,609 + GFF3038 0.50 +0.5
52,610 - GFF3038 0.50 +0.8
52,624 + GFF3038 0.52 +0.4
52,624 + GFF3038 0.52 -0.4
52,670 - GFF3038 0.58 +0.4
52,722 + GFF3038 0.65 +0.0
52,771 - GFF3038 0.72 +0.1
52,820 - GFF3038 0.78 +0.4

Or see this region's nucleotide sequence