Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF269

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF268 and GFF269 are separated by 85 nucleotidesGFF269 and GFF270 are separated by 253 nucleotides GFF268 - 3-polyprenyl-4-hydroxybenzoate carboxy-lyase (EC 4.1.1.-), at 42,432 to 43,910 GFF268 GFF269 - NAD(P)H-flavin reductase (EC 1.5.1.29) (EC 1.16.1.3), at 43,996 to 44,697 GFF269 GFF270 - Possible transferase, at 44,951 to 45,664 GFF270 Position (kb) 43 44 45Strain fitness (log2 ratio) -2 -1 0 1at 43.904 kb on + strandat 43.908 kb on + strandat 44.006 kb on + strandat 44.006 kb on + strandat 44.007 kb on - strandat 44.035 kb on - strandat 44.048 kb on + strandat 44.049 kb on - strandat 44.049 kb on - strandat 44.212 kb on + strand, within GFF269at 44.213 kb on - strand, within GFF269at 44.435 kb on - strand, within GFF269at 44.563 kb on + strand, within GFF269at 44.586 kb on + strand, within GFF269at 44.590 kb on + strand, within GFF269at 44.591 kb on - strand, within GFF269at 45.016 kb on + strandat 45.059 kb on + strand, within GFF270at 45.092 kb on - strand, within GFF270at 45.151 kb on - strand, within GFF270at 45.206 kb on - strand, within GFF270at 45.314 kb on - strand, within GFF270at 45.615 kb on + strandat 45.640 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
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43,904 + +1.7
43,908 + +0.4
44,006 + -1.1
44,006 + -1.3
44,007 - -0.0
44,035 - +0.9
44,048 + -0.7
44,049 - -0.6
44,049 - -0.5
44,212 + GFF269 0.31 -0.6
44,213 - GFF269 0.31 -0.8
44,435 - GFF269 0.63 +0.3
44,563 + GFF269 0.81 +0.5
44,586 + GFF269 0.84 +0.2
44,590 + GFF269 0.85 -1.4
44,591 - GFF269 0.85 -2.0
45,016 + +0.2
45,059 + GFF270 0.15 -0.4
45,092 - GFF270 0.20 -0.6
45,151 - GFF270 0.28 +1.0
45,206 - GFF270 0.36 +0.2
45,314 - GFF270 0.51 +0.2
45,615 + -0.1
45,640 + -0.0

Or see this region's nucleotide sequence