Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2413 and GFF2414 are separated by 291 nucleotides GFF2414 and GFF2415 are separated by 206 nucleotides GFF2415 and GFF2416 are separated by 120 nucleotides
GFF2413 - FIG021952: putative membrane protein, at 25,885 to 26,889
GFF2413
GFF2414 - Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-), at 27,181 to 28,347
GFF2414
GFF2415 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 28,554 to 28,985
GFF2415
GFF2416 - Putative polyferredoxin, at 29,106 to 29,969
GFF2416
Position (kb)
27
28
29 Strain fitness (log2 ratio)
-2
-1
0
1 at 26.907 kb on - strand at 26.977 kb on - strand at 26.995 kb on + strand at 26.995 kb on + strand at 26.996 kb on - strand at 26.996 kb on - strand at 26.996 kb on - strand at 26.996 kb on - strand at 27.057 kb on + strand at 27.060 kb on - strand at 27.060 kb on - strand at 27.064 kb on - strand at 27.128 kb on - strand at 27.128 kb on - strand at 27.152 kb on - strand at 27.180 kb on - strand at 27.187 kb on - strand at 27.216 kb on + strand at 27.241 kb on - strand at 27.308 kb on - strand, within GFF2414 at 27.418 kb on - strand, within GFF2414 at 27.423 kb on + strand, within GFF2414 at 27.423 kb on + strand, within GFF2414 at 27.424 kb on - strand, within GFF2414 at 27.501 kb on - strand, within GFF2414 at 27.501 kb on - strand, within GFF2414 at 27.501 kb on - strand, within GFF2414 at 27.582 kb on - strand, within GFF2414 at 27.634 kb on + strand, within GFF2414 at 27.634 kb on + strand, within GFF2414 at 27.634 kb on + strand, within GFF2414 at 27.634 kb on + strand, within GFF2414 at 27.635 kb on - strand, within GFF2414 at 27.635 kb on - strand, within GFF2414 at 27.658 kb on + strand, within GFF2414 at 27.658 kb on + strand, within GFF2414 at 27.788 kb on - strand, within GFF2414 at 28.014 kb on - strand, within GFF2414 at 28.124 kb on - strand, within GFF2414 at 28.188 kb on - strand, within GFF2414 at 28.188 kb on - strand, within GFF2414 at 28.347 kb on + strand at 28.347 kb on + strand at 28.347 kb on + strand at 28.347 kb on + strand at 28.348 kb on - strand at 28.370 kb on - strand at 28.376 kb on + strand at 28.419 kb on + strand at 28.461 kb on - strand at 28.511 kb on + strand at 28.658 kb on + strand, within GFF2415 at 28.659 kb on - strand, within GFF2415 at 28.659 kb on - strand, within GFF2415 at 28.659 kb on - strand, within GFF2415 at 28.773 kb on - strand, within GFF2415 at 28.834 kb on + strand, within GFF2415 at 28.834 kb on + strand, within GFF2415 at 28.990 kb on - strand at 29.031 kb on + strand at 29.094 kb on + strand at 29.094 kb on + strand at 29.207 kb on + strand, within GFF2416 at 29.208 kb on - strand, within GFF2416 at 29.217 kb on - strand, within GFF2416 at 29.220 kb on - strand, within GFF2416
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 26,907 - +0.7 26,977 - +0.7 26,995 + +0.1 26,995 + +0.1 26,996 - +0.2 26,996 - +0.3 26,996 - -0.8 26,996 - +0.5 27,057 + +0.1 27,060 - +0.2 27,060 - -0.1 27,064 - +0.1 27,128 - -0.1 27,128 - +0.6 27,152 - +1.5 27,180 - -0.3 27,187 - +0.1 27,216 + +1.3 27,241 - +0.4 27,308 - GFF2414 0.11 +0.8 27,418 - GFF2414 0.20 +0.7 27,423 + GFF2414 0.21 +0.5 27,423 + GFF2414 0.21 -0.6 27,424 - GFF2414 0.21 +0.1 27,501 - GFF2414 0.27 +0.8 27,501 - GFF2414 0.27 -1.1 27,501 - GFF2414 0.27 +0.4 27,582 - GFF2414 0.34 -0.2 27,634 + GFF2414 0.39 +0.2 27,634 + GFF2414 0.39 +0.2 27,634 + GFF2414 0.39 +0.2 27,634 + GFF2414 0.39 +0.3 27,635 - GFF2414 0.39 +0.5 27,635 - GFF2414 0.39 +1.1 27,658 + GFF2414 0.41 +0.1 27,658 + GFF2414 0.41 +0.9 27,788 - GFF2414 0.52 +0.3 28,014 - GFF2414 0.71 +0.4 28,124 - GFF2414 0.81 +0.1 28,188 - GFF2414 0.86 +0.3 28,188 - GFF2414 0.86 -0.1 28,347 + +0.1 28,347 + -0.2 28,347 + +1.4 28,347 + +1.2 28,348 - +0.5 28,370 - +0.3 28,376 + +0.2 28,419 + -0.1 28,461 - -0.6 28,511 + +0.2 28,658 + GFF2415 0.24 -0.3 28,659 - GFF2415 0.24 -1.5 28,659 - GFF2415 0.24 -1.5 28,659 - GFF2415 0.24 -1.5 28,773 - GFF2415 0.51 -1.9 28,834 + GFF2415 0.65 -1.3 28,834 + GFF2415 0.65 -1.0 28,990 - -0.2 29,031 + +0.3 29,094 + +0.2 29,094 + +0.3 29,207 + GFF2416 0.12 -2.1 29,208 - GFF2416 0.12 -0.5 29,217 - GFF2416 0.13 +1.2 29,220 - GFF2416 0.13 -1.1
Or see this region's nucleotide sequence