Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2384 and GFF2385 are separated by 40 nucleotides GFF2385 and GFF2386 are separated by 107 nucleotides GFF2386 and GFF2387 are separated by 10 nucleotides GFF2387 and GFF2388 are separated by 262 nucleotides GFF2388 and GFF2389 are separated by 141 nucleotides
GFF2384 - DNA-directed RNA polymerase alpha subunit (EC 2.7.7.6), at 14,074 to 15,063
GFF2384
GFF2385 - LSU ribosomal protein L17p, at 15,104 to 15,487
GFF2385
GFF2386 - FIG004614: Putative cytoplasmic protein, at 15,595 to 15,963
GFF2386
GFF2387 - transcriptional regulator, MerR family, at 15,974 to 16,399
GFF2387
GFF2388 - Large-conductance mechanosensitive channel, at 16,662 to 17,075
GFF2388
GFF2389 - Trk system potassium uptake protein TrkA, at 17,217 to 18,593
GFF2389
Position (kb) 15
16
17 Strain fitness (log2 ratio) -1
0
1 at 15.549 kb on + strand at 15.549 kb on + strand at 15.549 kb on + strand at 15.602 kb on + strand at 15.793 kb on - strand, within GFF2386 at 15.793 kb on - strand, within GFF2386 at 15.800 kb on + strand, within GFF2386 at 15.925 kb on + strand, within GFF2386 at 15.961 kb on + strand at 15.974 kb on - strand at 15.974 kb on - strand at 16.065 kb on + strand, within GFF2387 at 16.333 kb on + strand, within GFF2387 at 16.340 kb on + strand, within GFF2387 at 16.438 kb on + strand at 16.457 kb on - strand at 16.474 kb on + strand at 16.488 kb on + strand at 16.661 kb on - strand at 16.831 kb on - strand, within GFF2388 at 16.853 kb on - strand, within GFF2388 at 16.899 kb on - strand, within GFF2388 at 16.899 kb on - strand, within GFF2388
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 15,549 + -0.2 15,549 + +0.4 15,549 + +0.1 15,602 + -1.4 15,793 - GFF2386 0.54 -0.1 15,793 - GFF2386 0.54 +0.4 15,800 + GFF2386 0.56 -0.2 15,925 + GFF2386 0.89 -0.1 15,961 + +0.4 15,974 - +0.9 15,974 - -0.1 16,065 + GFF2387 0.21 -0.3 16,333 + GFF2387 0.84 +0.9 16,340 + GFF2387 0.86 +0.1 16,438 + +0.1 16,457 - +0.4 16,474 + +0.5 16,488 + +0.2 16,661 - +0.4 16,831 - GFF2388 0.41 +0.7 16,853 - GFF2388 0.46 +0.8 16,899 - GFF2388 0.57 +0.2 16,899 - GFF2388 0.57 +1.0
Or see this region's nucleotide sequence