Strain Fitness in Pseudomonas simiae WCS417 around PS417_12045

Experiment: 1-ethyl-3-methylimidazolium chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_12040 and PS417_12045 are separated by 56 nucleotidesPS417_12045 and PS417_12050 are separated by 27 nucleotidesPS417_12050 and PS417_12055 are separated by 87 nucleotides PS417_12040: PS417_12040 - dihydroxyacetone kinase, at 2,615,162 to 2,616,163 _12040 PS417_12045: PS417_12045 - iditol 2-dehydrogenase, at 2,616,220 to 2,617,335 _12045 PS417_12050: PS417_12050 - short-chain dehydrogenase, at 2,617,363 to 2,618,118 _12050 PS417_12055: PS417_12055 - xylitol ABC transporter, substrate-binding component (from data), at 2,618,206 to 2,619,213 _12055 Position (kb) 2616 2617 2618Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2615.278 kb on - strand, within PS417_12040at 2615.278 kb on - strandat 2615.278 kb on - strand, within PS417_12040at 2615.278 kb on - strand, within PS417_12040at 2615.289 kb on + strand, within PS417_12040at 2615.289 kb on + strand, within PS417_12040at 2615.290 kb on - strand, within PS417_12040at 2615.290 kb on - strandat 2615.290 kb on - strand, within PS417_12040at 2615.290 kb on - strand, within PS417_12040at 2615.290 kb on - strand, within PS417_12040at 2615.353 kb on - strand, within PS417_12040at 2615.353 kb on - strand, within PS417_12040at 2615.353 kb on - strand, within PS417_12040at 2615.486 kb on + strand, within PS417_12040at 2615.516 kb on + strandat 2615.658 kb on + strand, within PS417_12040at 2615.844 kb on + strand, within PS417_12040at 2615.845 kb on - strand, within PS417_12040at 2615.853 kb on + strand, within PS417_12040at 2615.973 kb on + strand, within PS417_12040at 2616.249 kb on + strandat 2616.371 kb on + strand, within PS417_12045at 2616.371 kb on + strand, within PS417_12045at 2616.372 kb on - strand, within PS417_12045at 2616.544 kb on - strand, within PS417_12045at 2616.881 kb on + strand, within PS417_12045at 2616.881 kb on + strand, within PS417_12045at 2616.881 kb on + strand, within PS417_12045at 2617.365 kb on - strandat 2617.436 kb on + strandat 2617.436 kb on + strandat 2617.607 kb on - strand, within PS417_12050at 2617.646 kb on + strand, within PS417_12050at 2617.853 kb on + strand, within PS417_12050at 2617.854 kb on - strand, within PS417_12050at 2617.900 kb on + strand, within PS417_12050at 2617.901 kb on - strand, within PS417_12050at 2618.123 kb on - strandat 2618.232 kb on - strandat 2618.232 kb on - strandat 2618.279 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 200 mM
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2,615,278 - PS417_12040 0.12 -0.2
2,615,278 - +1.0
2,615,278 - PS417_12040 0.12 +0.6
2,615,278 - PS417_12040 0.12 +1.5
2,615,289 + PS417_12040 0.13 +0.3
2,615,289 + PS417_12040 0.13 +1.1
2,615,290 - PS417_12040 0.13 -0.5
2,615,290 - -2.3
2,615,290 - PS417_12040 0.13 +0.2
2,615,290 - PS417_12040 0.13 +0.5
2,615,290 - PS417_12040 0.13 +0.2
2,615,353 - PS417_12040 0.19 -0.0
2,615,353 - PS417_12040 0.19 -0.2
2,615,353 - PS417_12040 0.19 +0.1
2,615,486 + PS417_12040 0.32 -1.0
2,615,516 + +3.7
2,615,658 + PS417_12040 0.50 -0.3
2,615,844 + PS417_12040 0.68 -0.8
2,615,845 - PS417_12040 0.68 -1.5
2,615,853 + PS417_12040 0.69 +1.0
2,615,973 + PS417_12040 0.81 +0.3
2,616,249 + -0.1
2,616,371 + PS417_12045 0.14 +2.3
2,616,371 + PS417_12045 0.14 -0.8
2,616,372 - PS417_12045 0.14 +0.2
2,616,544 - PS417_12045 0.29 +0.5
2,616,881 + PS417_12045 0.59 -0.6
2,616,881 + PS417_12045 0.59 +0.1
2,616,881 + PS417_12045 0.59 +0.1
2,617,365 - -0.7
2,617,436 + +0.3
2,617,436 + -0.7
2,617,607 - PS417_12050 0.32 +0.9
2,617,646 + PS417_12050 0.37 -0.2
2,617,853 + PS417_12050 0.65 -0.3
2,617,854 - PS417_12050 0.65 -0.1
2,617,900 + PS417_12050 0.71 +0.9
2,617,901 - PS417_12050 0.71 -1.1
2,618,123 - -0.1
2,618,232 - -1.1
2,618,232 - -0.2
2,618,279 + +0.4

Or see this region's nucleotide sequence