Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2263 and GFF2264 are separated by 142 nucleotides GFF2264 and GFF2265 overlap by 4 nucleotides
GFF2263 - Rhamnulose-1-phosphate aldolase (EC 4.1.2.19), at 54,006 to 54,833
GFF2263
GFF2264 - L-rhamnose isomerase (EC 5.3.1.14), at 54,976 to 56,235
GFF2264
GFF2265 - Rhamnulokinase (EC 2.7.1.5), at 56,232 to 57,701
GFF2265
Position (kb) 54
55
56
57 Strain fitness (log2 ratio) -1
0
1
2 at 54.004 kb on + strand at 54.005 kb on - strand at 54.123 kb on + strand, within GFF2263 at 54.124 kb on - strand, within GFF2263 at 54.147 kb on - strand, within GFF2263 at 54.176 kb on + strand, within GFF2263 at 54.177 kb on - strand, within GFF2263 at 54.379 kb on + strand, within GFF2263 at 54.380 kb on - strand, within GFF2263 at 54.399 kb on - strand, within GFF2263 at 54.417 kb on - strand, within GFF2263 at 54.749 kb on + strand, within GFF2263 at 54.750 kb on - strand, within GFF2263 at 54.822 kb on - strand at 54.849 kb on + strand at 54.879 kb on - strand at 54.982 kb on - strand at 55.007 kb on + strand at 55.065 kb on - strand at 55.065 kb on - strand at 55.131 kb on - strand, within GFF2264 at 55.400 kb on + strand, within GFF2264 at 55.400 kb on + strand, within GFF2264 at 55.400 kb on + strand, within GFF2264 at 55.502 kb on + strand, within GFF2264 at 55.527 kb on - strand, within GFF2264 at 55.600 kb on - strand, within GFF2264 at 55.943 kb on + strand, within GFF2264 at 56.175 kb on + strand at 56.175 kb on + strand at 56.176 kb on - strand at 56.176 kb on - strand at 56.180 kb on - strand at 56.314 kb on + strand at 56.314 kb on + strand at 56.315 kb on - strand at 56.333 kb on + strand at 56.334 kb on - strand at 56.370 kb on + strand at 56.388 kb on - strand, within GFF2265 at 56.397 kb on - strand, within GFF2265 at 56.403 kb on - strand, within GFF2265 at 56.403 kb on - strand, within GFF2265 at 56.567 kb on - strand, within GFF2265 at 56.941 kb on + strand, within GFF2265 at 56.941 kb on + strand, within GFF2265 at 56.941 kb on + strand, within GFF2265 at 56.942 kb on - strand, within GFF2265 at 56.954 kb on + strand, within GFF2265 at 57.215 kb on - strand, within GFF2265
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 54,004 + -0.2 54,005 - -0.1 54,123 + GFF2263 0.14 +0.3 54,124 - GFF2263 0.14 -0.5 54,147 - GFF2263 0.17 +0.1 54,176 + GFF2263 0.21 +0.2 54,177 - GFF2263 0.21 -0.2 54,379 + GFF2263 0.45 -1.7 54,380 - GFF2263 0.45 -0.4 54,399 - GFF2263 0.47 +1.9 54,417 - GFF2263 0.50 -0.1 54,749 + GFF2263 0.90 -0.1 54,750 - GFF2263 0.90 +0.6 54,822 - +0.0 54,849 + -0.5 54,879 - +1.0 54,982 - +0.6 55,007 + -0.8 55,065 - +0.2 55,065 - -0.3 55,131 - GFF2264 0.12 -0.8 55,400 + GFF2264 0.34 -0.4 55,400 + GFF2264 0.34 +0.5 55,400 + GFF2264 0.34 -0.8 55,502 + GFF2264 0.42 -0.9 55,527 - GFF2264 0.44 +0.1 55,600 - GFF2264 0.50 +0.8 55,943 + GFF2264 0.77 +0.5 56,175 + +0.4 56,175 + -0.3 56,176 - -1.5 56,176 - +0.6 56,180 - -0.5 56,314 + -0.1 56,314 + -0.2 56,315 - +0.2 56,333 + +0.4 56,334 - -0.1 56,370 + -0.1 56,388 - GFF2265 0.11 -0.0 56,397 - GFF2265 0.11 +0.1 56,403 - GFF2265 0.12 -0.2 56,403 - GFF2265 0.12 +0.3 56,567 - GFF2265 0.23 +0.2 56,941 + GFF2265 0.48 +0.5 56,941 + GFF2265 0.48 +0.5 56,941 + GFF2265 0.48 -0.8 56,942 - GFF2265 0.48 +0.2 56,954 + GFF2265 0.49 +0.5 57,215 - GFF2265 0.67 +0.1
Or see this region's nucleotide sequence