Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1693 and GFF1694 are separated by 20 nucleotides GFF1694 and GFF1695 overlap by 4 nucleotides GFF1695 and GFF1696 are separated by 231 nucleotides
GFF1693 - D-cysteine desulfhydrase (EC 4.4.1.15), at 2,645 to 3,631
GFF1693
GFF1694 - Cystine ABC transporter, permease protein, at 3,652 to 4,320
GFF1694
GFF1695 - Cystine ABC transporter, ATP-binding protein, at 4,317 to 5,069
GFF1695
GFF1696 - N-3-oxohexanoyl-L-homoserine lactone quorum-sensing transcriptional activator @ N-3-oxooctanoyl-L-homoserine lactone quorum-sensing transcriptional activator, at 5,301 to 6,023
GFF1696
Position (kb) 3
4
5 Strain fitness (log2 ratio) -1
0
1 at 2.652 kb on + strand at 2.750 kb on + strand, within GFF1693 at 3.104 kb on + strand, within GFF1693 at 3.153 kb on + strand, within GFF1693 at 3.249 kb on + strand, within GFF1693 at 3.355 kb on + strand, within GFF1693 at 3.400 kb on - strand, within GFF1693 at 3.411 kb on - strand, within GFF1693 at 3.628 kb on - strand at 3.628 kb on - strand at 3.628 kb on - strand at 3.823 kb on + strand, within GFF1694 at 3.823 kb on + strand, within GFF1694 at 3.824 kb on - strand, within GFF1694 at 3.874 kb on + strand, within GFF1694 at 3.878 kb on - strand, within GFF1694 at 4.110 kb on + strand, within GFF1694 at 4.128 kb on + strand, within GFF1694 at 4.128 kb on + strand, within GFF1694 at 4.181 kb on + strand, within GFF1694 at 4.182 kb on - strand, within GFF1694 at 4.209 kb on + strand, within GFF1694 at 4.453 kb on + strand, within GFF1695 at 4.454 kb on - strand, within GFF1695 at 4.497 kb on - strand, within GFF1695 at 4.959 kb on + strand, within GFF1695 at 5.202 kb on - strand at 5.225 kb on - strand at 5.225 kb on - strand at 5.227 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 2,652 + +0.2 2,750 + GFF1693 0.11 -0.0 3,104 + GFF1693 0.47 +0.8 3,153 + GFF1693 0.51 +0.0 3,249 + GFF1693 0.61 +0.0 3,355 + GFF1693 0.72 +0.8 3,400 - GFF1693 0.76 -0.7 3,411 - GFF1693 0.78 +0.2 3,628 - +0.4 3,628 - +0.2 3,628 - +0.6 3,823 + GFF1694 0.26 +1.3 3,823 + GFF1694 0.26 +0.1 3,824 - GFF1694 0.26 +0.7 3,874 + GFF1694 0.33 +0.3 3,878 - GFF1694 0.34 -0.2 4,110 + GFF1694 0.68 +0.3 4,128 + GFF1694 0.71 +0.1 4,128 + GFF1694 0.71 +0.7 4,181 + GFF1694 0.79 -0.2 4,182 - GFF1694 0.79 +0.3 4,209 + GFF1694 0.83 +0.8 4,453 + GFF1695 0.18 -0.5 4,454 - GFF1695 0.18 +0.7 4,497 - GFF1695 0.24 -0.3 4,959 + GFF1695 0.85 -0.3 5,202 - +0.1 5,225 - -0.4 5,225 - -0.1 5,227 - -0.1
Or see this region's nucleotide sequence