Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1635

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1634 and GFF1635 are separated by 116 nucleotidesGFF1635 and GFF1636 are separated by 15 nucleotides GFF1634 - Lipid A biosynthesis (KDO) 2-(lauroyl)-lipid IVA acyltransferase (EC 2.3.1.-), at 20,413 to 21,384 GFF1634 GFF1635 - Cell wall endopeptidase, family M23/M37, at 21,501 to 22,820 GFF1635 GFF1636 - Zinc ABC transporter, periplasmic-binding protein ZnuA, at 22,836 to 23,780 GFF1636 Position (kb) 21 22 23Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 20.625 kb on + strand, within GFF1634at 20.634 kb on - strand, within GFF1634at 20.634 kb on - strand, within GFF1634at 20.708 kb on + strand, within GFF1634at 20.709 kb on - strand, within GFF1634at 20.769 kb on - strand, within GFF1634at 20.874 kb on - strand, within GFF1634at 20.899 kb on - strand, within GFF1634at 21.357 kb on - strandat 21.425 kb on - strandat 21.429 kb on + strandat 21.547 kb on + strandat 21.630 kb on + strandat 21.631 kb on - strandat 21.745 kb on + strand, within GFF1635at 21.757 kb on + strand, within GFF1635at 21.970 kb on - strand, within GFF1635at 22.006 kb on + strand, within GFF1635at 22.040 kb on - strand, within GFF1635at 22.040 kb on - strand, within GFF1635at 22.040 kb on - strand, within GFF1635at 22.242 kb on - strand, within GFF1635at 22.242 kb on - strand, within GFF1635at 22.254 kb on - strand, within GFF1635at 22.331 kb on - strand, within GFF1635at 22.461 kb on - strand, within GFF1635at 22.463 kb on + strand, within GFF1635at 22.529 kb on - strand, within GFF1635at 22.583 kb on + strand, within GFF1635at 22.673 kb on + strand, within GFF1635at 22.822 kb on + strandat 22.873 kb on - strandat 22.905 kb on - strandat 23.131 kb on + strand, within GFF1636at 23.349 kb on - strand, within GFF1636at 23.371 kb on - strand, within GFF1636at 23.371 kb on - strand, within GFF1636at 23.484 kb on - strand, within GFF1636at 23.550 kb on + strand, within GFF1636at 23.790 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
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20,625 + GFF1634 0.22 -1.6
20,634 - GFF1634 0.23 -5.1
20,634 - GFF1634 0.23 -2.1
20,708 + GFF1634 0.30 -2.1
20,709 - GFF1634 0.30 -2.9
20,769 - GFF1634 0.37 -1.6
20,874 - GFF1634 0.47 -2.9
20,899 - GFF1634 0.50 -4.3
21,357 - -2.3
21,425 - +0.5
21,429 + -0.7
21,547 + +0.3
21,630 + -0.4
21,631 - +0.3
21,745 + GFF1635 0.18 +0.3
21,757 + GFF1635 0.19 +0.7
21,970 - GFF1635 0.36 +0.1
22,006 + GFF1635 0.38 -0.1
22,040 - GFF1635 0.41 -0.8
22,040 - GFF1635 0.41 -0.4
22,040 - GFF1635 0.41 +0.7
22,242 - GFF1635 0.56 +0.0
22,242 - GFF1635 0.56 +0.7
22,254 - GFF1635 0.57 +0.5
22,331 - GFF1635 0.63 +0.3
22,461 - GFF1635 0.73 -0.5
22,463 + GFF1635 0.73 -0.2
22,529 - GFF1635 0.78 +0.3
22,583 + GFF1635 0.82 -0.2
22,673 + GFF1635 0.89 -0.4
22,822 + -0.0
22,873 - -1.5
22,905 - +0.0
23,131 + GFF1636 0.31 +0.6
23,349 - GFF1636 0.54 +0.7
23,371 - GFF1636 0.57 +1.1
23,371 - GFF1636 0.57 -0.4
23,484 - GFF1636 0.69 +0.2
23,550 + GFF1636 0.76 +0.5
23,790 - +1.0

Or see this region's nucleotide sequence