Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1130 and GFF1131 are separated by 15 nucleotides GFF1131 and GFF1132 are separated by 21 nucleotides GFF1132 and GFF1133 are separated by 1 nucleotides
GFF1130 - Putative nitrite reductase, at 319,730 to 320,932
GFF1130
GFF1131 - Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1), at 320,948 to 321,868
GFF1131
GFF1132 - Transcriptional regulatory protein CitB, DpiA, at 321,890 to 322,576
GFF1132
GFF1133 - Sensor kinase CitA, DpiB (EC 2.7.3.-), at 322,578 to 324,236
GFF1133
Position (kb) 320
321
322 Strain fitness (log2 ratio) -2
-1
0
1
2 at 320.042 kb on - strand, within GFF1130 at 320.097 kb on - strand, within GFF1130 at 320.159 kb on + strand, within GFF1130 at 320.239 kb on - strand, within GFF1130 at 320.247 kb on + strand, within GFF1130 at 320.247 kb on + strand, within GFF1130 at 320.282 kb on - strand, within GFF1130 at 320.335 kb on - strand, within GFF1130 at 320.382 kb on - strand, within GFF1130 at 320.402 kb on + strand, within GFF1130 at 320.402 kb on + strand, within GFF1130 at 320.403 kb on - strand, within GFF1130 at 320.403 kb on - strand, within GFF1130 at 320.516 kb on + strand, within GFF1130 at 320.516 kb on + strand, within GFF1130 at 320.597 kb on + strand, within GFF1130 at 320.597 kb on + strand, within GFF1130 at 320.606 kb on + strand, within GFF1130 at 320.638 kb on + strand, within GFF1130 at 320.638 kb on + strand, within GFF1130 at 320.642 kb on + strand, within GFF1130 at 320.650 kb on - strand, within GFF1130 at 320.652 kb on + strand, within GFF1130 at 320.657 kb on - strand, within GFF1130 at 320.848 kb on + strand at 320.848 kb on + strand at 320.854 kb on - strand at 320.920 kb on + strand at 320.930 kb on + strand at 320.962 kb on - strand at 321.189 kb on - strand, within GFF1131 at 321.265 kb on + strand, within GFF1131 at 321.312 kb on + strand, within GFF1131 at 321.337 kb on + strand, within GFF1131 at 321.348 kb on + strand, within GFF1131 at 321.349 kb on - strand, within GFF1131 at 321.427 kb on + strand, within GFF1131 at 321.625 kb on + strand, within GFF1131 at 321.870 kb on + strand at 321.871 kb on - strand at 321.907 kb on - strand at 321.937 kb on - strand at 322.039 kb on + strand, within GFF1132 at 322.039 kb on + strand, within GFF1132 at 322.040 kb on - strand, within GFF1132 at 322.043 kb on + strand, within GFF1132 at 322.044 kb on - strand, within GFF1132 at 322.064 kb on + strand, within GFF1132 at 322.065 kb on - strand, within GFF1132 at 322.150 kb on + strand, within GFF1132 at 322.248 kb on + strand, within GFF1132 at 322.313 kb on - strand, within GFF1132 at 322.367 kb on - strand, within GFF1132 at 322.421 kb on + strand, within GFF1132 at 322.565 kb on - strand at 322.603 kb on + strand at 322.603 kb on + strand at 322.604 kb on - strand at 322.604 kb on - strand at 322.647 kb on - strand at 322.672 kb on + strand at 322.673 kb on - strand at 322.805 kb on + strand, within GFF1133
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 320,042 - GFF1130 0.26 +1.0 320,097 - GFF1130 0.31 +0.3 320,159 + GFF1130 0.36 +1.1 320,239 - GFF1130 0.42 +0.2 320,247 + GFF1130 0.43 -2.5 320,247 + GFF1130 0.43 +0.7 320,282 - GFF1130 0.46 +0.1 320,335 - GFF1130 0.50 -0.6 320,382 - GFF1130 0.54 +0.4 320,402 + GFF1130 0.56 -1.5 320,402 + GFF1130 0.56 -0.1 320,403 - GFF1130 0.56 -0.5 320,403 - GFF1130 0.56 +0.8 320,516 + GFF1130 0.65 +0.1 320,516 + GFF1130 0.65 -0.0 320,597 + GFF1130 0.72 -0.3 320,597 + GFF1130 0.72 +0.4 320,606 + GFF1130 0.73 -0.7 320,638 + GFF1130 0.75 +0.4 320,638 + GFF1130 0.75 +0.2 320,642 + GFF1130 0.76 +0.0 320,650 - GFF1130 0.76 -2.0 320,652 + GFF1130 0.77 +0.1 320,657 - GFF1130 0.77 +0.9 320,848 + +0.8 320,848 + +0.3 320,854 - +0.5 320,920 + +0.2 320,930 + +0.7 320,962 - -0.0 321,189 - GFF1131 0.26 +0.3 321,265 + GFF1131 0.34 +0.2 321,312 + GFF1131 0.40 -0.3 321,337 + GFF1131 0.42 -0.1 321,348 + GFF1131 0.43 -0.1 321,349 - GFF1131 0.44 +0.5 321,427 + GFF1131 0.52 +2.5 321,625 + GFF1131 0.74 +0.6 321,870 + +0.2 321,871 - -0.0 321,907 - -0.4 321,937 - +0.4 322,039 + GFF1132 0.22 +0.2 322,039 + GFF1132 0.22 -0.0 322,040 - GFF1132 0.22 +0.2 322,043 + GFF1132 0.22 -0.1 322,044 - GFF1132 0.22 -0.2 322,064 + GFF1132 0.25 +0.3 322,065 - GFF1132 0.25 +0.7 322,150 + GFF1132 0.38 +0.7 322,248 + GFF1132 0.52 -0.1 322,313 - GFF1132 0.62 +0.8 322,367 - GFF1132 0.69 -1.6 322,421 + GFF1132 0.77 +0.6 322,565 - +0.7 322,603 + +0.3 322,603 + +0.1 322,604 - +0.1 322,604 - +0.6 322,647 - +0.2 322,672 + +0.5 322,673 - -0.5 322,805 + GFF1133 0.14 -0.2
Or see this region's nucleotide sequence