Experiment: R2A with Fusidic 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4218 and Atu4219 overlap by 1 nucleotides Atu4219 and Atu4220 are separated by 29 nucleotides Atu4220 and Atu4221 are separated by 23 nucleotides
Atu4218: Atu4218 - multidrug efflux protein, at 1,339,962 to 1,341,071
Atu4218
Atu4219: Atu4219 - cation efflux system protein, at 1,341,071 to 1,344,181
Atu4219
Atu4220: Atu4220 - short-chain dehydrogenase, at 1,344,211 to 1,345,086
Atu4220
Atu4221: Atu4221 - hypothetical protein, at 1,345,110 to 1,345,658
Atu4221
Position (kb)
1341
1342
1343
1344
1345 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1340.077 kb on + strand, within Atu4218 at 1340.085 kb on + strand, within Atu4218 at 1340.093 kb on - strand, within Atu4218 at 1340.103 kb on - strand, within Atu4218 at 1340.120 kb on - strand, within Atu4218 at 1340.167 kb on - strand, within Atu4218 at 1340.184 kb on - strand, within Atu4218 at 1340.236 kb on - strand, within Atu4218 at 1340.237 kb on - strand, within Atu4218 at 1340.271 kb on - strand, within Atu4218 at 1340.288 kb on - strand, within Atu4218 at 1340.369 kb on - strand, within Atu4218 at 1340.378 kb on - strand, within Atu4218 at 1340.412 kb on - strand, within Atu4218 at 1340.428 kb on - strand, within Atu4218 at 1340.460 kb on - strand, within Atu4218 at 1340.503 kb on - strand, within Atu4218 at 1340.532 kb on - strand, within Atu4218 at 1340.552 kb on + strand, within Atu4218 at 1340.590 kb on - strand, within Atu4218 at 1340.604 kb on + strand, within Atu4218 at 1340.604 kb on + strand, within Atu4218 at 1340.684 kb on + strand, within Atu4218 at 1340.754 kb on + strand, within Atu4218 at 1340.760 kb on - strand, within Atu4218 at 1340.796 kb on + strand, within Atu4218 at 1340.804 kb on - strand, within Atu4218 at 1340.833 kb on + strand, within Atu4218 at 1340.856 kb on + strand, within Atu4218 at 1340.869 kb on - strand, within Atu4218 at 1340.880 kb on + strand, within Atu4218 at 1340.882 kb on + strand, within Atu4218 at 1340.882 kb on + strand, within Atu4218 at 1340.888 kb on - strand, within Atu4218 at 1340.900 kb on - strand, within Atu4218 at 1340.911 kb on - strand, within Atu4218 at 1340.918 kb on + strand, within Atu4218 at 1340.927 kb on + strand, within Atu4218 at 1340.939 kb on + strand, within Atu4218 at 1340.945 kb on - strand, within Atu4218 at 1340.951 kb on + strand, within Atu4218 at 1341.025 kb on - strand at 1341.075 kb on + strand at 1341.075 kb on + strand at 1341.083 kb on - strand at 1341.102 kb on - strand at 1341.209 kb on - strand at 1341.251 kb on + strand at 1341.259 kb on - strand at 1341.267 kb on - strand at 1341.298 kb on + strand at 1341.306 kb on - strand at 1341.446 kb on - strand, within Atu4219 at 1341.478 kb on + strand, within Atu4219 at 1341.508 kb on + strand, within Atu4219 at 1341.553 kb on - strand, within Atu4219 at 1341.568 kb on + strand, within Atu4219 at 1341.682 kb on + strand, within Atu4219 at 1341.755 kb on + strand, within Atu4219 at 1341.784 kb on - strand, within Atu4219 at 1341.793 kb on + strand, within Atu4219 at 1341.859 kb on + strand, within Atu4219 at 1341.954 kb on + strand, within Atu4219 at 1341.962 kb on - strand, within Atu4219 at 1341.962 kb on - strand, within Atu4219 at 1341.972 kb on - strand, within Atu4219 at 1341.985 kb on + strand, within Atu4219 at 1342.017 kb on + strand, within Atu4219 at 1342.106 kb on + strand, within Atu4219 at 1342.144 kb on - strand, within Atu4219 at 1342.280 kb on + strand, within Atu4219 at 1342.333 kb on + strand, within Atu4219 at 1342.333 kb on + strand, within Atu4219 at 1342.336 kb on - strand, within Atu4219 at 1342.414 kb on - strand, within Atu4219 at 1342.432 kb on + strand, within Atu4219 at 1342.521 kb on - strand, within Atu4219 at 1342.566 kb on - strand, within Atu4219 at 1342.580 kb on + strand, within Atu4219 at 1342.641 kb on - strand, within Atu4219 at 1342.714 kb on + strand, within Atu4219 at 1342.755 kb on - strand, within Atu4219 at 1342.776 kb on + strand, within Atu4219 at 1342.818 kb on + strand, within Atu4219 at 1342.831 kb on + strand, within Atu4219 at 1343.043 kb on - strand, within Atu4219 at 1343.049 kb on + strand, within Atu4219 at 1343.126 kb on + strand, within Atu4219 at 1343.145 kb on + strand, within Atu4219 at 1343.187 kb on + strand, within Atu4219 at 1343.263 kb on - strand, within Atu4219 at 1343.280 kb on + strand, within Atu4219 at 1343.298 kb on - strand, within Atu4219 at 1343.300 kb on - strand, within Atu4219 at 1343.369 kb on - strand, within Atu4219 at 1343.369 kb on - strand, within Atu4219 at 1343.548 kb on + strand, within Atu4219 at 1343.640 kb on - strand, within Atu4219 at 1343.773 kb on - strand, within Atu4219 at 1343.799 kb on - strand, within Atu4219 at 1343.811 kb on + strand, within Atu4219 at 1343.853 kb on + strand, within Atu4219 at 1343.865 kb on - strand, within Atu4219 at 1343.880 kb on - strand at 1343.935 kb on - strand at 1343.937 kb on + strand at 1343.937 kb on + strand at 1343.946 kb on + strand at 1344.057 kb on + strand at 1344.092 kb on + strand at 1344.092 kb on + strand at 1344.207 kb on + strand at 1344.232 kb on - strand at 1344.261 kb on + strand at 1344.411 kb on - strand, within Atu4220 at 1344.528 kb on - strand, within Atu4220 at 1344.538 kb on - strand, within Atu4220 at 1344.560 kb on - strand, within Atu4220 at 1344.616 kb on - strand, within Atu4220 at 1344.676 kb on - strand, within Atu4220 at 1344.748 kb on + strand, within Atu4220 at 1344.748 kb on + strand, within Atu4220 at 1344.748 kb on + strand, within Atu4220 at 1344.748 kb on + strand, within Atu4220 at 1344.756 kb on - strand, within Atu4220 at 1344.756 kb on - strand, within Atu4220 at 1344.756 kb on - strand, within Atu4220 at 1344.756 kb on - strand, within Atu4220 at 1344.842 kb on + strand, within Atu4220 at 1344.857 kb on - strand, within Atu4220 at 1344.874 kb on + strand, within Atu4220 at 1344.895 kb on + strand, within Atu4220 at 1344.903 kb on - strand, within Atu4220 at 1344.908 kb on - strand, within Atu4220 at 1344.913 kb on - strand, within Atu4220 at 1345.086 kb on + strand at 1345.094 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusidic 0.002 mM remove 1,340,077 + Atu4218 0.10 -0.9 1,340,085 + Atu4218 0.11 +0.3 1,340,093 - Atu4218 0.12 -0.6 1,340,103 - Atu4218 0.13 +0.4 1,340,120 - Atu4218 0.14 -0.4 1,340,167 - Atu4218 0.18 -0.3 1,340,184 - Atu4218 0.20 -0.8 1,340,236 - Atu4218 0.25 +0.2 1,340,237 - Atu4218 0.25 -0.0 1,340,271 - Atu4218 0.28 +1.3 1,340,288 - Atu4218 0.29 -0.1 1,340,369 - Atu4218 0.37 -0.6 1,340,378 - Atu4218 0.37 -0.0 1,340,412 - Atu4218 0.41 +0.1 1,340,428 - Atu4218 0.42 -0.1 1,340,460 - Atu4218 0.45 -0.8 1,340,503 - Atu4218 0.49 -1.1 1,340,532 - Atu4218 0.51 +1.0 1,340,552 + Atu4218 0.53 +0.1 1,340,590 - Atu4218 0.57 +0.6 1,340,604 + Atu4218 0.58 +0.5 1,340,604 + Atu4218 0.58 -0.1 1,340,684 + Atu4218 0.65 -0.5 1,340,754 + Atu4218 0.71 -0.5 1,340,760 - Atu4218 0.72 +0.1 1,340,796 + Atu4218 0.75 +0.5 1,340,804 - Atu4218 0.76 +0.9 1,340,833 + Atu4218 0.78 -1.3 1,340,856 + Atu4218 0.81 +0.2 1,340,869 - Atu4218 0.82 +0.2 1,340,880 + Atu4218 0.83 +0.6 1,340,882 + Atu4218 0.83 +0.5 1,340,882 + Atu4218 0.83 -0.0 1,340,888 - Atu4218 0.83 +0.3 1,340,900 - Atu4218 0.85 -0.5 1,340,911 - Atu4218 0.85 -0.3 1,340,918 + Atu4218 0.86 -0.0 1,340,927 + Atu4218 0.87 -0.3 1,340,939 + Atu4218 0.88 +0.9 1,340,945 - Atu4218 0.89 +0.3 1,340,951 + Atu4218 0.89 +0.2 1,341,025 - +0.5 1,341,075 + +0.1 1,341,075 + +0.0 1,341,083 - -0.1 1,341,102 - +0.2 1,341,209 - +0.2 1,341,251 + -0.2 1,341,259 - -0.1 1,341,267 - +0.3 1,341,298 + +0.2 1,341,306 - -0.1 1,341,446 - Atu4219 0.12 -0.3 1,341,478 + Atu4219 0.13 +1.3 1,341,508 + Atu4219 0.14 -1.2 1,341,553 - Atu4219 0.15 +0.4 1,341,568 + Atu4219 0.16 +0.7 1,341,682 + Atu4219 0.20 +0.2 1,341,755 + Atu4219 0.22 -1.9 1,341,784 - Atu4219 0.23 +0.7 1,341,793 + Atu4219 0.23 +1.2 1,341,859 + Atu4219 0.25 -0.3 1,341,954 + Atu4219 0.28 +1.0 1,341,962 - Atu4219 0.29 +0.2 1,341,962 - Atu4219 0.29 +0.3 1,341,972 - Atu4219 0.29 -1.1 1,341,985 + Atu4219 0.29 +0.1 1,342,017 + Atu4219 0.30 +0.2 1,342,106 + Atu4219 0.33 -0.4 1,342,144 - Atu4219 0.34 +0.0 1,342,280 + Atu4219 0.39 +0.2 1,342,333 + Atu4219 0.41 -0.1 1,342,333 + Atu4219 0.41 -1.6 1,342,336 - Atu4219 0.41 -0.1 1,342,414 - Atu4219 0.43 +1.0 1,342,432 + Atu4219 0.44 -0.5 1,342,521 - Atu4219 0.47 +0.2 1,342,566 - Atu4219 0.48 -0.4 1,342,580 + Atu4219 0.49 -0.8 1,342,641 - Atu4219 0.50 +0.4 1,342,714 + Atu4219 0.53 -0.1 1,342,755 - Atu4219 0.54 -0.8 1,342,776 + Atu4219 0.55 -0.1 1,342,818 + Atu4219 0.56 +0.1 1,342,831 + Atu4219 0.57 +1.1 1,343,043 - Atu4219 0.63 -0.2 1,343,049 + Atu4219 0.64 +0.5 1,343,126 + Atu4219 0.66 +0.2 1,343,145 + Atu4219 0.67 -0.1 1,343,187 + Atu4219 0.68 +0.2 1,343,263 - Atu4219 0.70 +0.3 1,343,280 + Atu4219 0.71 -1.8 1,343,298 - Atu4219 0.72 +0.5 1,343,300 - Atu4219 0.72 +0.1 1,343,369 - Atu4219 0.74 +0.0 1,343,369 - Atu4219 0.74 -0.1 1,343,548 + Atu4219 0.80 +1.5 1,343,640 - Atu4219 0.83 +0.2 1,343,773 - Atu4219 0.87 +0.1 1,343,799 - Atu4219 0.88 -0.4 1,343,811 + Atu4219 0.88 -0.5 1,343,853 + Atu4219 0.89 -0.4 1,343,865 - Atu4219 0.90 +0.1 1,343,880 - -0.4 1,343,935 - -0.5 1,343,937 + +0.3 1,343,937 + -0.1 1,343,946 + -0.1 1,344,057 + +1.1 1,344,092 + -0.4 1,344,092 + +1.0 1,344,207 + -0.2 1,344,232 - +0.2 1,344,261 + +0.1 1,344,411 - Atu4220 0.23 -0.7 1,344,528 - Atu4220 0.36 -1.1 1,344,538 - Atu4220 0.37 +0.1 1,344,560 - Atu4220 0.40 +0.5 1,344,616 - Atu4220 0.46 -0.2 1,344,676 - Atu4220 0.53 -0.0 1,344,748 + Atu4220 0.61 +1.6 1,344,748 + Atu4220 0.61 +0.1 1,344,748 + Atu4220 0.61 +1.0 1,344,748 + Atu4220 0.61 +0.4 1,344,756 - Atu4220 0.62 -0.8 1,344,756 - Atu4220 0.62 +0.2 1,344,756 - Atu4220 0.62 +0.1 1,344,756 - Atu4220 0.62 -0.2 1,344,842 + Atu4220 0.72 +0.7 1,344,857 - Atu4220 0.74 +0.2 1,344,874 + Atu4220 0.76 +0.1 1,344,895 + Atu4220 0.78 +1.2 1,344,903 - Atu4220 0.79 -0.6 1,344,908 - Atu4220 0.80 -0.6 1,344,913 - Atu4220 0.80 -0.4 1,345,086 + +2.0 1,345,094 - -0.4
Or see this region's nucleotide sequence