Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF940 and GFF941 are separated by 165 nucleotides GFF941 and GFF942 are separated by 59 nucleotides GFF942 and GFF943 are separated by 32 nucleotides
GFF940 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 115,099 to 116,103
GFF940
GFF941 - Ribonuclease E inhibitor RraB, at 116,269 to 116,685
GFF941
GFF942 - tRNA-(ms[2]io[6]A)-hydroxylase (EC 1.-.-.-), at 116,745 to 117,509
GFF942
GFF943 - FIG01046694: hypothetical protein, at 117,542 to 117,664
GFF943
Position (kb)
116
117 Strain fitness (log2 ratio)
-1
0
1 at 115.296 kb on + strand, within GFF940 at 115.344 kb on - strand, within GFF940 at 115.395 kb on + strand, within GFF940 at 115.419 kb on + strand, within GFF940 at 115.603 kb on - strand, within GFF940 at 115.603 kb on - strand, within GFF940 at 115.605 kb on + strand, within GFF940 at 115.706 kb on + strand, within GFF940 at 115.746 kb on - strand, within GFF940 at 115.846 kb on - strand, within GFF940 at 115.901 kb on + strand, within GFF940 at 116.103 kb on + strand at 116.104 kb on - strand at 116.104 kb on - strand at 116.104 kb on - strand at 116.185 kb on + strand at 116.286 kb on - strand at 116.286 kb on - strand at 116.362 kb on + strand, within GFF941 at 116.420 kb on + strand, within GFF941 at 116.441 kb on - strand, within GFF941 at 116.609 kb on - strand, within GFF941 at 116.650 kb on + strand at 116.694 kb on - strand at 116.697 kb on - strand at 116.740 kb on + strand at 116.744 kb on + strand at 116.914 kb on - strand, within GFF942 at 117.017 kb on + strand, within GFF942 at 117.159 kb on + strand, within GFF942 at 117.233 kb on - strand, within GFF942 at 117.233 kb on - strand, within GFF942 at 117.259 kb on - strand, within GFF942 at 117.259 kb on - strand, within GFF942 at 117.364 kb on - strand, within GFF942 at 117.372 kb on + strand, within GFF942 at 117.373 kb on - strand, within GFF942 at 117.373 kb on - strand, within GFF942 at 117.439 kb on + strand at 117.452 kb on + strand at 117.452 kb on + strand at 117.452 kb on + strand at 117.453 kb on - strand at 117.672 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 115,296 + GFF940 0.20 -0.1 115,344 - GFF940 0.24 -0.5 115,395 + GFF940 0.29 -0.2 115,419 + GFF940 0.32 +0.5 115,603 - GFF940 0.50 +0.8 115,603 - GFF940 0.50 +0.1 115,605 + GFF940 0.50 +0.4 115,706 + GFF940 0.60 +0.7 115,746 - GFF940 0.64 +0.7 115,846 - GFF940 0.74 +0.1 115,901 + GFF940 0.80 +0.2 116,103 + +0.6 116,104 - -0.7 116,104 - +0.6 116,104 - +0.2 116,185 + +0.1 116,286 - -0.1 116,286 - +0.2 116,362 + GFF941 0.22 -0.4 116,420 + GFF941 0.36 -0.7 116,441 - GFF941 0.41 -0.3 116,609 - GFF941 0.82 -1.0 116,650 + +0.9 116,694 - +0.3 116,697 - -0.7 116,740 + +0.1 116,744 + +0.0 116,914 - GFF942 0.22 -0.7 117,017 + GFF942 0.36 -0.1 117,159 + GFF942 0.54 +0.1 117,233 - GFF942 0.64 -0.7 117,233 - GFF942 0.64 +0.4 117,259 - GFF942 0.67 +0.6 117,259 - GFF942 0.67 -0.2 117,364 - GFF942 0.81 +0.7 117,372 + GFF942 0.82 -0.1 117,373 - GFF942 0.82 -0.9 117,373 - GFF942 0.82 +0.1 117,439 + +0.0 117,452 + +0.6 117,452 + -1.2 117,452 + +0.8 117,453 - -0.4 117,672 + +0.1
Or see this region's nucleotide sequence