Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF899 and GFF900 are separated by 72 nucleotides GFF900 and GFF901 are separated by 160 nucleotides
GFF899 - Myo-inositol 2-dehydrogenase 1 (EC 1.1.1.18), at 80,289 to 81,263
GFF899
GFF900 - alpha-ketoglutarate permease, at 81,336 to 82,562
GFF900
GFF901 - Inosose isomerase (EC 5.3.99.-), at 82,723 to 83,538
GFF901
Position (kb) 81
82
83 Strain fitness (log2 ratio) -2
-1
0
1 at 80.450 kb on + strand, within GFF899 at 80.951 kb on - strand, within GFF899 at 80.952 kb on + strand, within GFF899 at 80.952 kb on + strand, within GFF899 at 80.953 kb on - strand, within GFF899 at 80.953 kb on - strand, within GFF899 at 80.953 kb on - strand, within GFF899 at 81.015 kb on - strand, within GFF899 at 81.146 kb on + strand, within GFF899 at 81.146 kb on + strand, within GFF899 at 81.386 kb on + strand at 81.677 kb on + strand, within GFF900 at 81.681 kb on + strand, within GFF900 at 81.681 kb on + strand, within GFF900 at 81.682 kb on - strand, within GFF900 at 81.682 kb on - strand, within GFF900 at 81.683 kb on + strand, within GFF900 at 81.684 kb on - strand, within GFF900 at 81.718 kb on - strand, within GFF900 at 81.796 kb on - strand, within GFF900 at 81.796 kb on - strand, within GFF900 at 81.991 kb on - strand, within GFF900 at 81.995 kb on + strand, within GFF900 at 81.995 kb on + strand, within GFF900 at 81.999 kb on + strand, within GFF900 at 81.999 kb on + strand, within GFF900 at 82.000 kb on - strand, within GFF900 at 82.000 kb on - strand, within GFF900 at 82.044 kb on - strand, within GFF900 at 82.060 kb on - strand, within GFF900 at 82.079 kb on - strand, within GFF900 at 82.097 kb on + strand, within GFF900 at 82.140 kb on - strand, within GFF900 at 82.140 kb on - strand, within GFF900 at 82.140 kb on - strand, within GFF900 at 82.276 kb on + strand, within GFF900 at 82.277 kb on - strand, within GFF900 at 82.286 kb on + strand, within GFF900 at 82.286 kb on + strand, within GFF900 at 82.287 kb on - strand, within GFF900 at 82.290 kb on + strand, within GFF900 at 82.290 kb on + strand, within GFF900 at 82.290 kb on + strand, within GFF900 at 82.290 kb on + strand, within GFF900 at 82.290 kb on + strand, within GFF900 at 82.291 kb on - strand, within GFF900 at 82.301 kb on + strand, within GFF900 at 82.301 kb on + strand, within GFF900 at 82.493 kb on + strand at 82.493 kb on + strand at 82.542 kb on - strand at 82.705 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 80,450 + GFF899 0.17 -0.1 80,951 - GFF899 0.68 +0.1 80,952 + GFF899 0.68 +0.1 80,952 + GFF899 0.68 +0.2 80,953 - GFF899 0.68 -0.0 80,953 - GFF899 0.68 +0.5 80,953 - GFF899 0.68 -0.1 81,015 - GFF899 0.74 +0.1 81,146 + GFF899 0.88 -0.1 81,146 + GFF899 0.88 -1.0 81,386 + -0.0 81,677 + GFF900 0.28 -0.6 81,681 + GFF900 0.28 -0.1 81,681 + GFF900 0.28 -0.0 81,682 - GFF900 0.28 -0.1 81,682 - GFF900 0.28 -0.6 81,683 + GFF900 0.28 +0.5 81,684 - GFF900 0.28 +0.2 81,718 - GFF900 0.31 +0.8 81,796 - GFF900 0.37 -1.8 81,796 - GFF900 0.37 -0.1 81,991 - GFF900 0.53 +0.3 81,995 + GFF900 0.54 -0.1 81,995 + GFF900 0.54 +0.5 81,999 + GFF900 0.54 +0.3 81,999 + GFF900 0.54 +0.3 82,000 - GFF900 0.54 +0.6 82,000 - GFF900 0.54 -0.6 82,044 - GFF900 0.58 -0.3 82,060 - GFF900 0.59 +0.2 82,079 - GFF900 0.61 -0.4 82,097 + GFF900 0.62 -0.0 82,140 - GFF900 0.66 -0.6 82,140 - GFF900 0.66 -0.0 82,140 - GFF900 0.66 -0.2 82,276 + GFF900 0.77 -1.1 82,277 - GFF900 0.77 -0.9 82,286 + GFF900 0.77 -0.2 82,286 + GFF900 0.77 +0.7 82,287 - GFF900 0.78 +0.5 82,290 + GFF900 0.78 -0.4 82,290 + GFF900 0.78 -1.7 82,290 + GFF900 0.78 -0.1 82,290 + GFF900 0.78 -0.1 82,290 + GFF900 0.78 -0.3 82,291 - GFF900 0.78 -0.0 82,301 + GFF900 0.79 -0.6 82,301 + GFF900 0.79 -0.1 82,493 + -0.0 82,493 + +0.2 82,542 - -0.3 82,705 - +0.7
Or see this region's nucleotide sequence