Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF506 and GFF507 are separated by 12 nucleotides GFF507 and GFF508 are separated by 325 nucleotides
GFF506 - Putative inner membrane protein, at 234,253 to 235,842
GFF506
GFF507 - Cytoplasmic trehalase (EC 3.2.1.28), at 235,855 to 237,504
GFF507
GFF508 - Transcriptional regulator, GntR family, at 237,830 to 238,549
GFF508
Position (kb) 235
236
237
238 Strain fitness (log2 ratio) -1
0
1 at 234.881 kb on - strand, within GFF506 at 234.881 kb on - strand, within GFF506 at 235.017 kb on + strand, within GFF506 at 235.017 kb on + strand, within GFF506 at 235.018 kb on - strand, within GFF506 at 235.018 kb on - strand, within GFF506 at 235.018 kb on - strand, within GFF506 at 235.025 kb on + strand, within GFF506 at 235.182 kb on + strand, within GFF506 at 235.182 kb on + strand, within GFF506 at 235.182 kb on + strand, within GFF506 at 235.183 kb on - strand, within GFF506 at 235.183 kb on - strand, within GFF506 at 235.205 kb on + strand, within GFF506 at 235.206 kb on - strand, within GFF506 at 235.206 kb on - strand, within GFF506 at 235.206 kb on - strand, within GFF506 at 235.218 kb on + strand, within GFF506 at 235.257 kb on + strand, within GFF506 at 235.429 kb on - strand, within GFF506 at 235.438 kb on - strand, within GFF506 at 235.483 kb on - strand, within GFF506 at 235.563 kb on + strand, within GFF506 at 235.643 kb on + strand, within GFF506 at 235.644 kb on - strand, within GFF506 at 235.644 kb on - strand, within GFF506 at 235.686 kb on + strand at 235.746 kb on + strand at 235.795 kb on - strand at 235.825 kb on + strand at 235.969 kb on - strand at 236.189 kb on - strand, within GFF507 at 236.294 kb on + strand, within GFF507 at 236.295 kb on - strand, within GFF507 at 236.295 kb on - strand, within GFF507 at 236.330 kb on + strand, within GFF507 at 236.413 kb on - strand, within GFF507 at 236.483 kb on - strand, within GFF507 at 236.747 kb on + strand, within GFF507 at 236.856 kb on - strand, within GFF507 at 236.877 kb on + strand, within GFF507 at 236.885 kb on + strand, within GFF507 at 236.925 kb on + strand, within GFF507 at 236.925 kb on + strand, within GFF507 at 236.925 kb on + strand, within GFF507 at 236.925 kb on + strand, within GFF507 at 236.925 kb on + strand, within GFF507 at 236.929 kb on + strand, within GFF507 at 236.967 kb on + strand, within GFF507 at 236.968 kb on - strand, within GFF507 at 236.968 kb on - strand, within GFF507 at 237.020 kb on + strand, within GFF507 at 237.020 kb on + strand, within GFF507 at 237.020 kb on + strand, within GFF507 at 237.020 kb on + strand, within GFF507 at 237.021 kb on - strand, within GFF507 at 237.103 kb on - strand, within GFF507 at 237.137 kb on + strand, within GFF507 at 237.219 kb on + strand, within GFF507 at 237.308 kb on - strand, within GFF507 at 237.330 kb on - strand, within GFF507 at 237.459 kb on + strand at 237.460 kb on - strand at 237.740 kb on - strand at 237.792 kb on + strand at 237.869 kb on + strand at 237.871 kb on + strand at 237.918 kb on - strand, within GFF508 at 238.110 kb on + strand, within GFF508 at 238.111 kb on - strand, within GFF508 at 238.235 kb on - strand, within GFF508 at 238.275 kb on + strand, within GFF508 at 238.322 kb on - strand, within GFF508 at 238.334 kb on + strand, within GFF508 at 238.335 kb on - strand, within GFF508 at 238.335 kb on - strand, within GFF508 at 238.335 kb on - strand, within GFF508 at 238.362 kb on + strand, within GFF508 at 238.362 kb on + strand, within GFF508
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 234,881 - GFF506 0.39 -0.0 234,881 - GFF506 0.39 -0.0 235,017 + GFF506 0.48 +0.4 235,017 + GFF506 0.48 +0.1 235,018 - GFF506 0.48 -0.3 235,018 - GFF506 0.48 -0.8 235,018 - GFF506 0.48 +0.4 235,025 + GFF506 0.49 +0.1 235,182 + GFF506 0.58 +0.3 235,182 + GFF506 0.58 +0.3 235,182 + GFF506 0.58 +0.2 235,183 - GFF506 0.58 +0.2 235,183 - GFF506 0.58 +1.0 235,205 + GFF506 0.60 +0.1 235,206 - GFF506 0.60 +1.5 235,206 - GFF506 0.60 -1.3 235,206 - GFF506 0.60 -0.0 235,218 + GFF506 0.61 +0.9 235,257 + GFF506 0.63 -0.0 235,429 - GFF506 0.74 +0.4 235,438 - GFF506 0.75 +0.1 235,483 - GFF506 0.77 -0.0 235,563 + GFF506 0.82 +0.9 235,643 + GFF506 0.87 -0.0 235,644 - GFF506 0.87 +0.0 235,644 - GFF506 0.87 +1.1 235,686 + +0.0 235,746 + +0.1 235,795 - +0.2 235,825 + +0.9 235,969 - -0.2 236,189 - GFF507 0.20 +0.3 236,294 + GFF507 0.27 +0.9 236,295 - GFF507 0.27 +0.2 236,295 - GFF507 0.27 +0.3 236,330 + GFF507 0.29 +0.4 236,413 - GFF507 0.34 +0.3 236,483 - GFF507 0.38 +0.2 236,747 + GFF507 0.54 -0.2 236,856 - GFF507 0.61 -0.4 236,877 + GFF507 0.62 -0.4 236,885 + GFF507 0.62 -0.1 236,925 + GFF507 0.65 +0.0 236,925 + GFF507 0.65 +0.4 236,925 + GFF507 0.65 -0.5 236,925 + GFF507 0.65 -0.0 236,925 + GFF507 0.65 -0.1 236,929 + GFF507 0.65 -0.8 236,967 + GFF507 0.67 -0.4 236,968 - GFF507 0.67 -0.3 236,968 - GFF507 0.67 +0.1 237,020 + GFF507 0.71 -0.1 237,020 + GFF507 0.71 -0.1 237,020 + GFF507 0.71 -0.3 237,020 + GFF507 0.71 +0.3 237,021 - GFF507 0.71 +0.5 237,103 - GFF507 0.76 +0.2 237,137 + GFF507 0.78 +0.8 237,219 + GFF507 0.83 -0.7 237,308 - GFF507 0.88 +0.1 237,330 - GFF507 0.89 -0.1 237,459 + -0.5 237,460 - -0.3 237,740 - +0.2 237,792 + +0.9 237,869 + -0.8 237,871 + -0.2 237,918 - GFF508 0.12 -0.5 238,110 + GFF508 0.39 -0.1 238,111 - GFF508 0.39 -1.2 238,235 - GFF508 0.56 -0.2 238,275 + GFF508 0.62 -0.8 238,322 - GFF508 0.68 -0.2 238,334 + GFF508 0.70 -0.2 238,335 - GFF508 0.70 -0.4 238,335 - GFF508 0.70 -0.6 238,335 - GFF508 0.70 -0.0 238,362 + GFF508 0.74 -0.4 238,362 + GFF508 0.74 -1.6
Or see this region's nucleotide sequence