Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4912 and GFF4913 are separated by 10 nucleotides GFF4913 and GFF4914 are separated by 95 nucleotides
GFF4912 - Low-specificity L-threonine aldolase (EC 4.1.2.5), at 15,008 to 16,009
GFF4912
GFF4913 - FIG00638862: hypothetical protein, at 16,020 to 17,453
GFF4913
GFF4914 - Putative nucleotide di-P-sugar epimerase or dehydratase, at 17,549 to 18,562
GFF4914
Position (kb) 16
17
18 Strain fitness (log2 ratio) -1
0
1 at 15.029 kb on - strand at 15.094 kb on - strand at 15.094 kb on - strand at 15.235 kb on + strand, within GFF4912 at 15.256 kb on + strand, within GFF4912 at 15.260 kb on + strand, within GFF4912 at 15.260 kb on + strand, within GFF4912 at 15.261 kb on - strand, within GFF4912 at 15.261 kb on - strand, within GFF4912 at 15.449 kb on + strand, within GFF4912 at 15.450 kb on - strand, within GFF4912 at 15.473 kb on - strand, within GFF4912 at 15.473 kb on - strand, within GFF4912 at 15.549 kb on + strand, within GFF4912 at 15.550 kb on - strand, within GFF4912 at 15.567 kb on - strand, within GFF4912 at 15.671 kb on - strand, within GFF4912 at 15.725 kb on + strand, within GFF4912 at 15.726 kb on - strand, within GFF4912 at 16.233 kb on + strand, within GFF4913 at 16.233 kb on + strand, within GFF4913 at 16.338 kb on - strand, within GFF4913 at 16.509 kb on + strand, within GFF4913 at 16.509 kb on + strand, within GFF4913 at 16.510 kb on - strand, within GFF4913 at 16.585 kb on - strand, within GFF4913 at 16.769 kb on + strand, within GFF4913 at 17.186 kb on + strand, within GFF4913 at 17.528 kb on + strand at 17.556 kb on + strand at 17.777 kb on - strand, within GFF4914 at 17.882 kb on - strand, within GFF4914 at 18.045 kb on + strand, within GFF4914 at 18.082 kb on - strand, within GFF4914 at 18.082 kb on - strand, within GFF4914 at 18.104 kb on - strand, within GFF4914 at 18.117 kb on - strand, within GFF4914 at 18.167 kb on + strand, within GFF4914 at 18.167 kb on + strand, within GFF4914 at 18.243 kb on - strand, within GFF4914 at 18.243 kb on - strand, within GFF4914 at 18.243 kb on - strand, within GFF4914 at 18.274 kb on - strand, within GFF4914 at 18.286 kb on - strand, within GFF4914 at 18.353 kb on - strand, within GFF4914 at 18.445 kb on + strand, within GFF4914
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 15,029 - -0.2 15,094 - +0.3 15,094 - -0.1 15,235 + GFF4912 0.23 +0.3 15,256 + GFF4912 0.25 -0.2 15,260 + GFF4912 0.25 -0.0 15,260 + GFF4912 0.25 +0.6 15,261 - GFF4912 0.25 +0.6 15,261 - GFF4912 0.25 +0.2 15,449 + GFF4912 0.44 +0.1 15,450 - GFF4912 0.44 +0.0 15,473 - GFF4912 0.46 +0.5 15,473 - GFF4912 0.46 -0.0 15,549 + GFF4912 0.54 +0.3 15,550 - GFF4912 0.54 +0.5 15,567 - GFF4912 0.56 -0.0 15,671 - GFF4912 0.66 +0.5 15,725 + GFF4912 0.72 +0.6 15,726 - GFF4912 0.72 +0.7 16,233 + GFF4913 0.15 +0.2 16,233 + GFF4913 0.15 +0.7 16,338 - GFF4913 0.22 +0.7 16,509 + GFF4913 0.34 +0.2 16,509 + GFF4913 0.34 +0.0 16,510 - GFF4913 0.34 +1.5 16,585 - GFF4913 0.39 +0.1 16,769 + GFF4913 0.52 +0.4 17,186 + GFF4913 0.81 -0.0 17,528 + -1.0 17,556 + +0.0 17,777 - GFF4914 0.22 +0.1 17,882 - GFF4914 0.33 +0.3 18,045 + GFF4914 0.49 +0.3 18,082 - GFF4914 0.53 +0.0 18,082 - GFF4914 0.53 -0.0 18,104 - GFF4914 0.55 -0.8 18,117 - GFF4914 0.56 -1.2 18,167 + GFF4914 0.61 +0.2 18,167 + GFF4914 0.61 +0.5 18,243 - GFF4914 0.68 -0.2 18,243 - GFF4914 0.68 +0.7 18,243 - GFF4914 0.68 -0.0 18,274 - GFF4914 0.71 +0.4 18,286 - GFF4914 0.73 +0.0 18,353 - GFF4914 0.79 +0.1 18,445 + GFF4914 0.88 -0.6
Or see this region's nucleotide sequence