Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4889 and GFF4890 are separated by 44 nucleotides GFF4890 and GFF4891 are separated by 0 nucleotides
GFF4889 - Leucyl/phenylalanyl-tRNA--protein transferase (EC 2.3.2.6), at 5,199 to 5,903
GFF4889
GFF4890 - Transport ATP-binding protein CydC, at 5,948 to 7,669
GFF4890
GFF4891 - Transport ATP-binding protein CydD, at 7,670 to 9,436
GFF4891
Position (kb) 5
6
7
8 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 5.069 kb on + strand at 5.080 kb on + strand at 5.080 kb on + strand at 5.081 kb on - strand at 5.081 kb on - strand at 5.082 kb on + strand at 5.083 kb on - strand at 5.083 kb on - strand at 5.083 kb on - strand at 5.113 kb on - strand at 5.135 kb on - strand at 5.168 kb on - strand at 5.470 kb on + strand, within GFF4889 at 5.558 kb on + strand, within GFF4889 at 5.788 kb on - strand, within GFF4889 at 5.941 kb on + strand at 5.949 kb on + strand at 6.122 kb on + strand, within GFF4890 at 6.274 kb on + strand, within GFF4890 at 6.274 kb on + strand, within GFF4890 at 6.887 kb on + strand, within GFF4890 at 6.887 kb on + strand, within GFF4890 at 6.888 kb on - strand, within GFF4890 at 6.888 kb on - strand, within GFF4890 at 7.204 kb on + strand, within GFF4890 at 7.358 kb on + strand, within GFF4890 at 7.359 kb on - strand, within GFF4890 at 7.377 kb on + strand, within GFF4890 at 7.492 kb on + strand, within GFF4890 at 7.493 kb on - strand, within GFF4890 at 7.495 kb on - strand, within GFF4890 at 7.495 kb on - strand, within GFF4890 at 7.617 kb on - strand at 7.627 kb on + strand at 7.651 kb on - strand at 7.672 kb on - strand at 7.672 kb on - strand at 8.012 kb on + strand, within GFF4891 at 8.108 kb on + strand, within GFF4891 at 8.136 kb on - strand, within GFF4891 at 8.233 kb on - strand, within GFF4891 at 8.249 kb on + strand, within GFF4891 at 8.252 kb on + strand, within GFF4891 at 8.369 kb on - strand, within GFF4891 at 8.372 kb on - strand, within GFF4891 at 8.372 kb on - strand, within GFF4891 at 8.372 kb on - strand, within GFF4891 at 8.372 kb on - strand, within GFF4891 at 8.470 kb on + strand, within GFF4891 at 8.497 kb on + strand, within GFF4891 at 8.506 kb on - strand, within GFF4891 at 8.657 kb on - strand, within GFF4891
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 5,069 + +0.2 5,080 + +0.2 5,080 + +1.2 5,081 - -0.4 5,081 - -0.1 5,082 + +0.4 5,083 - +0.4 5,083 - -0.1 5,083 - +0.1 5,113 - -1.2 5,135 - +0.1 5,168 - -1.5 5,470 + GFF4889 0.38 -0.2 5,558 + GFF4889 0.51 +0.7 5,788 - GFF4889 0.84 +0.5 5,941 + -0.3 5,949 + +0.3 6,122 + GFF4890 0.10 -1.6 6,274 + GFF4890 0.19 -4.4 6,274 + GFF4890 0.19 -3.9 6,887 + GFF4890 0.55 -5.8 6,887 + GFF4890 0.55 -2.7 6,888 - GFF4890 0.55 -4.9 6,888 - GFF4890 0.55 -2.8 7,204 + GFF4890 0.73 -3.4 7,358 + GFF4890 0.82 -3.6 7,359 - GFF4890 0.82 -0.8 7,377 + GFF4890 0.83 -1.2 7,492 + GFF4890 0.90 -2.2 7,493 - GFF4890 0.90 -0.2 7,495 - GFF4890 0.90 -2.7 7,495 - GFF4890 0.90 -3.0 7,617 - -2.6 7,627 + -5.2 7,651 - -4.2 7,672 - -0.4 7,672 - +0.2 8,012 + GFF4891 0.19 -3.3 8,108 + GFF4891 0.25 -3.2 8,136 - GFF4891 0.26 -4.4 8,233 - GFF4891 0.32 -2.1 8,249 + GFF4891 0.33 -5.2 8,252 + GFF4891 0.33 -0.2 8,369 - GFF4891 0.40 -2.9 8,372 - GFF4891 0.40 -1.2 8,372 - GFF4891 0.40 -2.1 8,372 - GFF4891 0.40 -3.4 8,372 - GFF4891 0.40 -4.3 8,470 + GFF4891 0.45 -3.7 8,497 + GFF4891 0.47 -4.0 8,506 - GFF4891 0.47 -3.8 8,657 - GFF4891 0.56 -0.8
Or see this region's nucleotide sequence