Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4806 and GFF4807 are separated by 4 nucleotides GFF4807 and GFF4808 are separated by 91 nucleotides GFF4808 and GFF4809 overlap by 1 nucleotides GFF4809 and GFF4810 overlap by 4 nucleotides
GFF4806 - FIG01045727: hypothetical protein, at 107,266 to 107,457
GFF4806
GFF4807 - CRISPR associated protein of unknown function, at 107,462 to 108,118
GFF4807
GFF4808 - CRISPR-associated protein, Cas2, at 108,210 to 108,503
GFF4808
GFF4809 - CRISPR-associated protein Cas1, at 108,503 to 109,423
GFF4809
GFF4810 - CRISPR-associated protein, Cse3 family, at 109,420 to 110,070
GFF4810
Position (kb)
108
109 Strain fitness (log2 ratio)
-1
0
1 at 107.212 kb on + strand at 107.212 kb on + strand at 107.213 kb on - strand at 107.324 kb on + strand, within GFF4806 at 107.400 kb on + strand, within GFF4806 at 108.066 kb on + strand at 108.127 kb on - strand at 108.127 kb on - strand at 108.269 kb on + strand, within GFF4808 at 108.269 kb on + strand, within GFF4808 at 108.314 kb on + strand, within GFF4808 at 108.413 kb on - strand, within GFF4808 at 108.471 kb on + strand, within GFF4808 at 108.498 kb on - strand at 108.498 kb on - strand at 108.806 kb on - strand, within GFF4809 at 108.886 kb on + strand, within GFF4809 at 108.886 kb on + strand, within GFF4809 at 108.886 kb on + strand, within GFF4809 at 108.887 kb on - strand, within GFF4809 at 108.887 kb on - strand, within GFF4809 at 108.930 kb on + strand, within GFF4809 at 108.930 kb on + strand, within GFF4809 at 108.931 kb on - strand, within GFF4809 at 108.934 kb on + strand, within GFF4809 at 108.978 kb on + strand, within GFF4809 at 109.006 kb on + strand, within GFF4809 at 109.271 kb on - strand, within GFF4809 at 109.271 kb on - strand, within GFF4809 at 109.295 kb on - strand, within GFF4809 at 109.359 kb on + strand at 109.383 kb on - strand at 109.477 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 107,212 + -0.3 107,212 + +0.4 107,213 - +0.0 107,324 + GFF4806 0.30 +1.0 107,400 + GFF4806 0.70 -0.1 108,066 + +0.4 108,127 - +0.3 108,127 - +0.2 108,269 + GFF4808 0.20 +0.3 108,269 + GFF4808 0.20 +1.1 108,314 + GFF4808 0.35 +1.3 108,413 - GFF4808 0.69 +0.1 108,471 + GFF4808 0.89 +0.3 108,498 - +0.7 108,498 - -0.3 108,806 - GFF4809 0.33 +0.1 108,886 + GFF4809 0.42 +0.0 108,886 + GFF4809 0.42 -0.0 108,886 + GFF4809 0.42 +0.1 108,887 - GFF4809 0.42 -0.1 108,887 - GFF4809 0.42 +1.3 108,930 + GFF4809 0.46 +0.9 108,930 + GFF4809 0.46 +1.7 108,931 - GFF4809 0.46 +0.4 108,934 + GFF4809 0.47 +0.2 108,978 + GFF4809 0.52 +0.3 109,006 + GFF4809 0.55 -0.0 109,271 - GFF4809 0.83 -0.1 109,271 - GFF4809 0.83 -0.5 109,295 - GFF4809 0.86 +0.4 109,359 + +0.3 109,383 - -0.6 109,477 - +0.0
Or see this region's nucleotide sequence