Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF436 and GFF437 are separated by 116 nucleotides GFF437 and GFF438 are separated by 36 nucleotides GFF438 and GFF439 are separated by 173 nucleotides
GFF436 - Transcriptional regulator, IclR family, at 157,669 to 158,469
GFF436
GFF437 - Electron transport protein HydN, at 158,586 to 159,059
GFF437
GFF438 - Valine--pyruvate aminotransferase (EC 2.6.1.66), at 159,096 to 160,346
GFF438
GFF439 - Periplasmic alpha-amylase (EC 3.2.1.1), at 160,520 to 162,547
GFF439
Position (kb) 159
160
161 Strain fitness (log2 ratio) -1
0
1 at 158.101 kb on + strand, within GFF436 at 158.101 kb on + strand, within GFF436 at 158.128 kb on - strand, within GFF436 at 158.205 kb on + strand, within GFF436 at 158.205 kb on + strand, within GFF436 at 158.206 kb on - strand, within GFF436 at 158.230 kb on + strand, within GFF436 at 158.230 kb on + strand, within GFF436 at 158.231 kb on - strand, within GFF436 at 158.292 kb on - strand, within GFF436 at 158.305 kb on - strand, within GFF436 at 158.439 kb on + strand at 158.569 kb on - strand at 158.597 kb on + strand at 158.735 kb on + strand, within GFF437 at 158.871 kb on - strand, within GFF437 at 159.121 kb on - strand at 159.121 kb on - strand at 159.175 kb on + strand at 159.175 kb on + strand at 159.175 kb on + strand at 159.176 kb on - strand at 159.236 kb on - strand, within GFF438 at 159.286 kb on - strand, within GFF438 at 159.416 kb on - strand, within GFF438 at 159.418 kb on + strand, within GFF438 at 159.467 kb on + strand, within GFF438 at 159.555 kb on - strand, within GFF438 at 159.618 kb on + strand, within GFF438 at 159.618 kb on + strand, within GFF438 at 159.618 kb on + strand, within GFF438 at 159.618 kb on + strand, within GFF438 at 159.619 kb on - strand, within GFF438 at 159.847 kb on + strand, within GFF438 at 159.847 kb on + strand, within GFF438 at 159.848 kb on - strand, within GFF438 at 159.848 kb on - strand, within GFF438 at 159.848 kb on - strand, within GFF438 at 159.851 kb on + strand, within GFF438 at 159.957 kb on + strand, within GFF438 at 159.960 kb on + strand, within GFF438 at 159.971 kb on + strand, within GFF438 at 160.004 kb on + strand, within GFF438 at 160.008 kb on + strand, within GFF438 at 160.143 kb on - strand, within GFF438 at 160.178 kb on + strand, within GFF438 at 160.178 kb on + strand, within GFF438 at 160.388 kb on + strand at 160.563 kb on + strand at 160.652 kb on - strand at 160.654 kb on + strand at 160.654 kb on + strand at 160.654 kb on + strand at 160.655 kb on - strand at 160.780 kb on - strand, within GFF439 at 161.010 kb on + strand, within GFF439 at 161.010 kb on + strand, within GFF439 at 161.068 kb on + strand, within GFF439 at 161.290 kb on - strand, within GFF439 at 161.318 kb on - strand, within GFF439
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 158,101 + GFF436 0.54 -0.3 158,101 + GFF436 0.54 -0.2 158,128 - GFF436 0.57 +0.1 158,205 + GFF436 0.67 -0.6 158,205 + GFF436 0.67 +0.3 158,206 - GFF436 0.67 -0.5 158,230 + GFF436 0.70 +0.3 158,230 + GFF436 0.70 +0.2 158,231 - GFF436 0.70 -0.2 158,292 - GFF436 0.78 -0.6 158,305 - GFF436 0.79 -0.2 158,439 + +0.2 158,569 - -0.2 158,597 + +0.6 158,735 + GFF437 0.31 +0.1 158,871 - GFF437 0.60 +0.1 159,121 - +0.7 159,121 - +0.6 159,175 + +0.5 159,175 + -0.1 159,175 + -1.2 159,176 - -0.0 159,236 - GFF438 0.11 -1.2 159,286 - GFF438 0.15 -0.4 159,416 - GFF438 0.26 -0.3 159,418 + GFF438 0.26 -0.3 159,467 + GFF438 0.30 +0.1 159,555 - GFF438 0.37 -0.3 159,618 + GFF438 0.42 +0.1 159,618 + GFF438 0.42 -1.2 159,618 + GFF438 0.42 +0.5 159,618 + GFF438 0.42 -0.3 159,619 - GFF438 0.42 -0.0 159,847 + GFF438 0.60 -1.7 159,847 + GFF438 0.60 -0.1 159,848 - GFF438 0.60 +0.3 159,848 - GFF438 0.60 +0.3 159,848 - GFF438 0.60 -0.2 159,851 + GFF438 0.60 +0.7 159,957 + GFF438 0.69 -0.3 159,960 + GFF438 0.69 -0.2 159,971 + GFF438 0.70 +0.0 160,004 + GFF438 0.73 +0.5 160,008 + GFF438 0.73 -0.6 160,143 - GFF438 0.84 +0.4 160,178 + GFF438 0.86 +0.4 160,178 + GFF438 0.86 +0.5 160,388 + +0.0 160,563 + -0.9 160,652 - +0.1 160,654 + -0.0 160,654 + -0.3 160,654 + +0.5 160,655 - +0.5 160,780 - GFF439 0.13 -0.1 161,010 + GFF439 0.24 +0.3 161,010 + GFF439 0.24 +0.5 161,068 + GFF439 0.27 -0.1 161,290 - GFF439 0.38 +0.1 161,318 - GFF439 0.39 -0.3
Or see this region's nucleotide sequence