Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4288 and GFF4289 are separated by 70 nucleotides GFF4289 and GFF4290 are separated by 37 nucleotides GFF4290 and GFF4291 are separated by 125 nucleotides
GFF4288 - AroM protein, at 72,071 to 72,748
GFF4288
GFF4289 - Cytoplasmic protein YaiE, at 72,819 to 73,103
GFF4289
GFF4290 - DNA recombination-dependent growth factor C, at 73,141 to 74,052
GFF4290
GFF4291 - Glucokinase, ROK family (EC 2.7.1.2), at 74,178 to 75,086
GFF4291
Position (kb) 73
74
75 Strain fitness (log2 ratio) -1
0
1
2 at 72.172 kb on + strand, within GFF4288 at 72.352 kb on + strand, within GFF4288 at 72.355 kb on - strand, within GFF4288 at 72.398 kb on + strand, within GFF4288 at 72.421 kb on + strand, within GFF4288 at 72.582 kb on + strand, within GFF4288 at 72.707 kb on - strand at 72.780 kb on - strand at 72.780 kb on - strand at 72.854 kb on + strand, within GFF4289 at 72.855 kb on - strand, within GFF4289 at 72.921 kb on + strand, within GFF4289 at 72.922 kb on - strand, within GFF4289 at 72.924 kb on - strand, within GFF4289 at 72.924 kb on - strand, within GFF4289 at 73.014 kb on - strand, within GFF4289 at 73.083 kb on + strand at 73.084 kb on - strand at 73.257 kb on + strand, within GFF4290 at 73.374 kb on + strand, within GFF4290 at 73.605 kb on + strand, within GFF4290 at 73.605 kb on + strand, within GFF4290 at 74.005 kb on - strand at 74.025 kb on - strand at 74.091 kb on + strand at 74.175 kb on + strand at 74.310 kb on - strand, within GFF4291 at 74.388 kb on + strand, within GFF4291 at 74.417 kb on + strand, within GFF4291 at 74.552 kb on - strand, within GFF4291 at 74.596 kb on + strand, within GFF4291 at 74.707 kb on - strand, within GFF4291 at 74.784 kb on - strand, within GFF4291 at 75.020 kb on - strand at 75.023 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 72,172 + GFF4288 0.15 -0.9 72,352 + GFF4288 0.41 -1.4 72,355 - GFF4288 0.42 -1.3 72,398 + GFF4288 0.48 -0.4 72,421 + GFF4288 0.52 +0.2 72,582 + GFF4288 0.75 -0.9 72,707 - -1.0 72,780 - +0.1 72,780 - -0.4 72,854 + GFF4289 0.12 +0.6 72,855 - GFF4289 0.13 +1.2 72,921 + GFF4289 0.36 -0.3 72,922 - GFF4289 0.36 -0.4 72,924 - GFF4289 0.37 +2.4 72,924 - GFF4289 0.37 -0.2 73,014 - GFF4289 0.68 -0.0 73,083 + -0.6 73,084 - +0.0 73,257 + GFF4290 0.13 +0.1 73,374 + GFF4290 0.26 +0.9 73,605 + GFF4290 0.51 -0.3 73,605 + GFF4290 0.51 +0.3 74,005 - +0.0 74,025 - +0.1 74,091 + -0.2 74,175 + +0.1 74,310 - GFF4291 0.15 -1.1 74,388 + GFF4291 0.23 -1.0 74,417 + GFF4291 0.26 +0.1 74,552 - GFF4291 0.41 +0.8 74,596 + GFF4291 0.46 +1.4 74,707 - GFF4291 0.58 -0.2 74,784 - GFF4291 0.67 -0.0 75,020 - +0.2 75,023 + -1.5
Or see this region's nucleotide sequence