Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4045

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4044 and GFF4045 are separated by 88 nucleotidesGFF4045 and GFF4046 overlap by 4 nucleotidesGFF4046 and GFF4047 are separated by 54 nucleotides GFF4044 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 6,772 to 7,527 GFF4044 GFF4045 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 7,616 to 8,284 GFF4045 GFF4046 - Ribonuclease HI (EC 3.1.26.4), at 8,281 to 8,748 GFF4046 GFF4047 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 8,803 to 9,543 GFF4047 Position (kb) 7 8 9Strain fitness (log2 ratio) -2 -1 0 1at 6.681 kb on - strandat 6.721 kb on - strandat 6.721 kb on - strandat 6.763 kb on + strandat 6.952 kb on - strand, within GFF4044at 7.017 kb on + strand, within GFF4044at 7.017 kb on + strand, within GFF4044at 7.018 kb on - strand, within GFF4044at 7.022 kb on - strand, within GFF4044at 7.245 kb on + strand, within GFF4044at 7.246 kb on - strand, within GFF4044at 7.401 kb on + strand, within GFF4044at 7.402 kb on - strand, within GFF4044at 7.402 kb on - strand, within GFF4044at 7.513 kb on - strandat 7.527 kb on + strandat 7.528 kb on - strandat 7.530 kb on - strandat 7.532 kb on + strandat 7.631 kb on + strandat 7.806 kb on + strand, within GFF4045at 7.806 kb on + strand, within GFF4045at 7.855 kb on + strand, within GFF4045at 7.856 kb on - strand, within GFF4045at 8.073 kb on + strand, within GFF4045at 8.074 kb on - strand, within GFF4045at 8.074 kb on - strand, within GFF4045at 8.079 kb on - strand, within GFF4045at 8.079 kb on - strand, within GFF4045at 8.097 kb on + strand, within GFF4045at 8.098 kb on - strand, within GFF4045at 8.164 kb on + strand, within GFF4045at 8.165 kb on - strand, within GFF4045at 8.216 kb on - strand, within GFF4045at 8.283 kb on - strandat 8.304 kb on + strandat 8.800 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
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6,681 - -1.0
6,721 - +0.3
6,721 - -0.2
6,763 + +0.1
6,952 - GFF4044 0.24 +0.0
7,017 + GFF4044 0.32 +0.8
7,017 + GFF4044 0.32 +0.2
7,018 - GFF4044 0.33 +0.2
7,022 - GFF4044 0.33 +0.7
7,245 + GFF4044 0.63 +0.0
7,246 - GFF4044 0.63 -2.5
7,401 + GFF4044 0.83 +0.6
7,402 - GFF4044 0.83 -0.1
7,402 - GFF4044 0.83 +0.8
7,513 - -0.0
7,527 + +0.1
7,528 - +0.5
7,530 - -0.0
7,532 + +0.9
7,631 + -0.6
7,806 + GFF4045 0.28 +0.0
7,806 + GFF4045 0.28 +0.3
7,855 + GFF4045 0.36 -0.5
7,856 - GFF4045 0.36 -0.1
8,073 + GFF4045 0.68 -0.3
8,074 - GFF4045 0.68 -0.7
8,074 - GFF4045 0.68 -0.5
8,079 - GFF4045 0.69 -0.3
8,079 - GFF4045 0.69 -0.7
8,097 + GFF4045 0.72 -0.4
8,098 - GFF4045 0.72 +0.6
8,164 + GFF4045 0.82 -0.4
8,165 - GFF4045 0.82 -0.6
8,216 - GFF4045 0.90 -0.7
8,283 - +0.5
8,304 + +0.6
8,800 + -1.2

Or see this region's nucleotide sequence