Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF335 and GFF336 are separated by 228 nucleotides GFF336 and GFF337 are separated by 54 nucleotides GFF337 and GFF338 are separated by 696 nucleotides
GFF335 - Putative inner membrane protein, at 51,803 to 52,330
GFF335
GFF336 - Nicotinamidase family protein YcaC, at 52,559 to 53,239
GFF336
GFF337 - hypothetical protein, at 53,294 to 53,419
GFF337
GFF338 - Putative sensory transducer, at 54,116 to 54,880
GFF338
Position (kb) 52
53
54 Strain fitness (log2 ratio) -2
-1
0
1 at 51.592 kb on + strand at 51.625 kb on + strand at 51.626 kb on - strand at 51.778 kb on - strand at 51.896 kb on - strand, within GFF335 at 52.000 kb on + strand, within GFF335 at 52.073 kb on - strand, within GFF335 at 52.073 kb on - strand, within GFF335 at 52.076 kb on + strand, within GFF335 at 52.076 kb on + strand, within GFF335 at 52.076 kb on + strand, within GFF335 at 52.077 kb on - strand, within GFF335 at 52.077 kb on - strand, within GFF335 at 52.122 kb on - strand, within GFF335 at 52.131 kb on + strand, within GFF335 at 52.131 kb on + strand, within GFF335 at 52.211 kb on - strand, within GFF335 at 52.211 kb on - strand, within GFF335 at 52.225 kb on - strand, within GFF335 at 52.242 kb on - strand, within GFF335 at 52.265 kb on - strand, within GFF335 at 52.329 kb on - strand at 52.526 kb on - strand at 52.557 kb on - strand at 52.561 kb on - strand at 52.589 kb on - strand at 52.657 kb on + strand, within GFF336 at 52.659 kb on + strand, within GFF336 at 52.661 kb on + strand, within GFF336 at 52.661 kb on + strand, within GFF336 at 52.661 kb on + strand, within GFF336 at 52.661 kb on + strand, within GFF336 at 52.661 kb on + strand, within GFF336 at 52.661 kb on + strand, within GFF336 at 52.661 kb on + strand, within GFF336 at 52.739 kb on + strand, within GFF336 at 52.740 kb on - strand, within GFF336 at 52.740 kb on - strand, within GFF336 at 52.742 kb on + strand, within GFF336 at 52.743 kb on - strand, within GFF336 at 52.794 kb on + strand, within GFF336 at 52.823 kb on + strand, within GFF336 at 52.824 kb on - strand, within GFF336 at 52.824 kb on - strand, within GFF336 at 52.879 kb on + strand, within GFF336 at 52.882 kb on + strand, within GFF336 at 52.980 kb on + strand, within GFF336 at 52.980 kb on + strand, within GFF336 at 52.980 kb on + strand, within GFF336 at 53.069 kb on - strand, within GFF336 at 53.075 kb on + strand, within GFF336 at 53.075 kb on + strand, within GFF336 at 53.239 kb on + strand at 53.240 kb on - strand at 53.240 kb on - strand at 53.686 kb on + strand at 53.687 kb on - strand at 53.961 kb on + strand at 53.962 kb on - strand at 53.977 kb on - strand at 53.995 kb on + strand at 54.004 kb on + strand at 54.054 kb on - strand at 54.086 kb on + strand at 54.127 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 51,592 + +0.4 51,625 + +0.2 51,626 - -0.6 51,778 - +0.2 51,896 - GFF335 0.18 +0.7 52,000 + GFF335 0.37 +0.3 52,073 - GFF335 0.51 +0.1 52,073 - GFF335 0.51 +0.3 52,076 + GFF335 0.52 -0.5 52,076 + GFF335 0.52 -0.2 52,076 + GFF335 0.52 +0.7 52,077 - GFF335 0.52 +0.1 52,077 - GFF335 0.52 +1.0 52,122 - GFF335 0.60 +1.1 52,131 + GFF335 0.62 +0.2 52,131 + GFF335 0.62 +1.3 52,211 - GFF335 0.77 -0.4 52,211 - GFF335 0.77 -0.0 52,225 - GFF335 0.80 +0.2 52,242 - GFF335 0.83 -0.4 52,265 - GFF335 0.88 -1.4 52,329 - -0.1 52,526 - +0.1 52,557 - +0.2 52,561 - -0.2 52,589 - -0.3 52,657 + GFF336 0.14 -1.8 52,659 + GFF336 0.15 -0.3 52,661 + GFF336 0.15 +0.2 52,661 + GFF336 0.15 -0.2 52,661 + GFF336 0.15 -1.1 52,661 + GFF336 0.15 +0.3 52,661 + GFF336 0.15 -0.4 52,661 + GFF336 0.15 -0.1 52,661 + GFF336 0.15 +0.1 52,739 + GFF336 0.26 -1.3 52,740 - GFF336 0.27 +0.4 52,740 - GFF336 0.27 +0.0 52,742 + GFF336 0.27 -0.4 52,743 - GFF336 0.27 -0.0 52,794 + GFF336 0.35 +0.5 52,823 + GFF336 0.39 -1.7 52,824 - GFF336 0.39 +0.7 52,824 - GFF336 0.39 +0.1 52,879 + GFF336 0.47 -0.9 52,882 + GFF336 0.47 +0.4 52,980 + GFF336 0.62 -0.1 52,980 + GFF336 0.62 -0.4 52,980 + GFF336 0.62 +0.4 53,069 - GFF336 0.75 -0.1 53,075 + GFF336 0.76 -0.9 53,075 + GFF336 0.76 -0.1 53,239 + -0.5 53,240 - +0.2 53,240 - -0.2 53,686 + +0.2 53,687 - -0.3 53,961 + +0.8 53,962 - -0.6 53,977 - +0.1 53,995 + -0.7 54,004 + +0.2 54,054 - -0.3 54,086 + -0.5 54,127 - +0.0
Or see this region's nucleotide sequence