Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF328

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF326 and GFF327 overlap by 4 nucleotidesGFF327 and GFF328 are separated by 3 nucleotidesGFF328 and GFF329 overlap by 14 nucleotidesGFF329 and GFF330 are separated by 235 nucleotides GFF326 - PTS system, mannose-specific IIC component, at 41,815 to 42,564 GFF326 GFF327 - PTS system, mannose-specific IID component (EC 2.7.1.69), at 42,561 to 43,421 GFF327 GFF328 - L-seryl-tRNA(Sec) selenium transferase-related protein, at 43,425 to 44,534 GFF328 GFF329 - 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16), at 44,521 to 45,264 GFF329 GFF330 - Transposase, at 45,500 to 46,441 GFF330 Position (kb) 43 44 45Strain fitness (log2 ratio) -3 -2 -1 0 1at 42.577 kb on + strandat 42.577 kb on + strandat 42.578 kb on - strandat 42.578 kb on - strandat 42.654 kb on - strand, within GFF327at 42.688 kb on - strand, within GFF327at 42.697 kb on + strand, within GFF327at 42.791 kb on + strand, within GFF327at 42.800 kb on + strand, within GFF327at 42.842 kb on + strand, within GFF327at 43.072 kb on - strand, within GFF327at 43.094 kb on + strand, within GFF327at 43.094 kb on + strand, within GFF327at 43.094 kb on + strand, within GFF327at 43.094 kb on + strand, within GFF327at 43.095 kb on - strand, within GFF327at 43.095 kb on - strand, within GFF327at 43.095 kb on - strand, within GFF327at 43.206 kb on + strand, within GFF327at 43.207 kb on - strand, within GFF327at 43.326 kb on + strand, within GFF327at 43.332 kb on + strand, within GFF327at 43.394 kb on - strandat 43.419 kb on + strandat 43.419 kb on + strandat 43.420 kb on - strandat 43.437 kb on - strandat 43.437 kb on - strandat 43.607 kb on + strand, within GFF328at 43.622 kb on + strand, within GFF328at 43.623 kb on - strand, within GFF328at 43.623 kb on - strand, within GFF328at 43.623 kb on - strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.626 kb on + strand, within GFF328at 43.627 kb on - strand, within GFF328at 43.627 kb on - strand, within GFF328at 43.627 kb on - strand, within GFF328at 43.627 kb on - strand, within GFF328at 43.742 kb on - strand, within GFF328at 43.760 kb on + strand, within GFF328at 44.045 kb on + strand, within GFF328at 44.049 kb on + strand, within GFF328at 44.050 kb on - strand, within GFF328at 44.050 kb on - strand, within GFF328at 44.070 kb on - strand, within GFF328at 44.099 kb on + strand, within GFF328at 44.099 kb on + strand, within GFF328at 44.099 kb on + strand, within GFF328at 44.190 kb on + strand, within GFF328at 44.191 kb on - strand, within GFF328at 44.600 kb on - strand, within GFF329at 44.600 kb on - strand, within GFF329at 44.600 kb on - strand, within GFF329at 44.600 kb on - strand, within GFF329at 44.600 kb on - strand, within GFF329at 44.657 kb on + strand, within GFF329at 44.657 kb on + strand, within GFF329at 44.743 kb on + strand, within GFF329at 44.743 kb on + strand, within GFF329at 44.746 kb on + strand, within GFF329at 44.773 kb on - strand, within GFF329at 45.041 kb on + strand, within GFF329at 45.041 kb on + strand, within GFF329at 45.041 kb on + strand, within GFF329at 45.042 kb on - strand, within GFF329at 45.245 kb on + strandat 45.246 kb on - strandat 45.313 kb on + strandat 45.314 kb on - strandat 45.314 kb on - strandat 45.382 kb on - strandat 45.382 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
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42,577 + -0.8
42,577 + -0.0
42,578 - +0.2
42,578 - +0.3
42,654 - GFF327 0.11 -0.7
42,688 - GFF327 0.15 +0.1
42,697 + GFF327 0.16 -0.0
42,791 + GFF327 0.27 -0.0
42,800 + GFF327 0.28 +0.9
42,842 + GFF327 0.33 +0.3
43,072 - GFF327 0.59 -0.5
43,094 + GFF327 0.62 -0.0
43,094 + GFF327 0.62 +0.3
43,094 + GFF327 0.62 -0.3
43,094 + GFF327 0.62 +0.6
43,095 - GFF327 0.62 -0.9
43,095 - GFF327 0.62 +0.3
43,095 - GFF327 0.62 +0.9
43,206 + GFF327 0.75 +0.4
43,207 - GFF327 0.75 -1.0
43,326 + GFF327 0.89 -0.1
43,332 + GFF327 0.90 -0.2
43,394 - +0.1
43,419 + -0.0
43,419 + -0.0
43,420 - +0.0
43,437 - -0.4
43,437 - -3.7
43,607 + GFF328 0.16 -0.2
43,622 + GFF328 0.18 -1.0
43,623 - GFF328 0.18 -0.1
43,623 - GFF328 0.18 +0.5
43,623 - GFF328 0.18 +0.3
43,626 + GFF328 0.18 +0.2
43,626 + GFF328 0.18 +0.3
43,626 + GFF328 0.18 +0.6
43,626 + GFF328 0.18 +1.0
43,626 + GFF328 0.18 -1.7
43,626 + GFF328 0.18 +0.3
43,626 + GFF328 0.18 -0.0
43,626 + GFF328 0.18 -0.1
43,627 - GFF328 0.18 +0.5
43,627 - GFF328 0.18 -0.2
43,627 - GFF328 0.18 -0.0
43,627 - GFF328 0.18 +0.2
43,742 - GFF328 0.29 -0.3
43,760 + GFF328 0.30 -0.5
44,045 + GFF328 0.56 -0.1
44,049 + GFF328 0.56 -0.2
44,050 - GFF328 0.56 -0.6
44,050 - GFF328 0.56 +0.3
44,070 - GFF328 0.58 -1.3
44,099 + GFF328 0.61 +0.0
44,099 + GFF328 0.61 -0.3
44,099 + GFF328 0.61 +0.0
44,190 + GFF328 0.69 -0.4
44,191 - GFF328 0.69 -1.2
44,600 - GFF329 0.11 +0.4
44,600 - GFF329 0.11 +0.2
44,600 - GFF329 0.11 -1.5
44,600 - GFF329 0.11 +0.0
44,600 - GFF329 0.11 -0.0
44,657 + GFF329 0.18 -0.0
44,657 + GFF329 0.18 -0.0
44,743 + GFF329 0.30 -0.5
44,743 + GFF329 0.30 +0.2
44,746 + GFF329 0.30 -0.0
44,773 - GFF329 0.34 +0.1
45,041 + GFF329 0.70 -0.0
45,041 + GFF329 0.70 -0.3
45,041 + GFF329 0.70 +0.6
45,042 - GFF329 0.70 +0.4
45,245 + +0.3
45,246 - +0.2
45,313 + +0.0
45,314 - -0.5
45,314 - +0.8
45,382 - -0.2
45,382 - +0.1

Or see this region's nucleotide sequence