Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3233

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3231 and GFF3232 overlap by 19 nucleotidesGFF3232 and GFF3233 are separated by 221 nucleotidesGFF3233 and GFF3234 are separated by 270 nucleotidesGFF3234 and GFF3235 are separated by 426 nucleotides GFF3231 - Gifsy-2 prophage protein, at 3,453 to 4,079 GFF3231 GFF3232 - hypothetical protein, at 4,061 to 4,210 GFF3232 GFF3233 - FIG01046696: hypothetical protein, at 4,432 to 5,118 GFF3233 GFF3234 - Virulence protein msgA, at 5,389 to 5,580 GFF3234 GFF3235 - Membrane alanine aminopeptidase N (EC 3.4.11.2), at 6,007 to 8,619 GFF3235 Position (kb) 4 5 6Strain fitness (log2 ratio) -1 0 1at 3.455 kb on - strandat 3.455 kb on - strandat 3.635 kb on + strand, within GFF3231at 3.819 kb on - strand, within GFF3231at 3.919 kb on - strand, within GFF3231at 3.953 kb on + strand, within GFF3231at 3.984 kb on - strand, within GFF3231at 3.997 kb on + strand, within GFF3231at 3.998 kb on - strand, within GFF3231at 3.998 kb on - strand, within GFF3231at 4.005 kb on - strand, within GFF3231at 4.007 kb on + strand, within GFF3231at 4.040 kb on - strandat 4.080 kb on - strand, within GFF3232at 4.500 kb on - strandat 5.581 kb on - strandat 5.904 kb on + strandat 5.963 kb on + strandat 5.983 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
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3,455 - -0.5
3,455 - +0.4
3,635 + GFF3231 0.29 -0.3
3,819 - GFF3231 0.58 +0.4
3,919 - GFF3231 0.74 +0.1
3,953 + GFF3231 0.80 +0.0
3,984 - GFF3231 0.85 -0.2
3,997 + GFF3231 0.87 -1.0
3,998 - GFF3231 0.87 +0.2
3,998 - GFF3231 0.87 -0.0
4,005 - GFF3231 0.88 -0.5
4,007 + GFF3231 0.88 -1.0
4,040 - +1.1
4,080 - GFF3232 0.13 +0.4
4,500 - -0.3
5,581 - +0.5
5,904 + -0.5
5,963 + +0.4
5,983 - +0.0

Or see this region's nucleotide sequence