Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3147

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3145 and GFF3146 overlap by 1 nucleotidesGFF3146 and GFF3147 are separated by 24 nucleotidesGFF3147 and GFF3148 overlap by 4 nucleotidesGFF3148 and GFF3149 are separated by 206 nucleotides GFF3145 - Putative esterase, FIGfam005057, at 151,017 to 151,598 GFF3145 GFF3146 - '3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17)' transl_table=11, at 151,598 to 152,425 GFF3146 GFF3147 - FIG074102: hypothetical protein, at 152,450 to 152,872 GFF3147 GFF3148 - ADP-ribose pyrophosphatase (EC 3.6.1.13), at 152,869 to 153,501 GFF3148 GFF3149 - Type I secretion outer membrane protein, TolC precursor, at 153,708 to 155,177 GFF3149 Position (kb) 152 153Strain fitness (log2 ratio) -2 -1 0 1at 151.715 kb on - strand, within GFF3146at 151.816 kb on + strand, within GFF3146at 152.436 kb on + strandat 152.568 kb on + strand, within GFF3147at 152.664 kb on + strand, within GFF3147at 152.664 kb on + strand, within GFF3147at 152.704 kb on + strand, within GFF3147at 152.744 kb on + strand, within GFF3147at 153.007 kb on - strand, within GFF3148at 153.037 kb on - strand, within GFF3148at 153.076 kb on - strand, within GFF3148at 153.076 kb on - strand, within GFF3148at 153.113 kb on + strand, within GFF3148at 153.205 kb on + strand, within GFF3148at 153.522 kb on - strandat 153.529 kb on - strandat 153.529 kb on - strandat 153.603 kb on - strandat 153.684 kb on - strandat 153.763 kb on + strandat 153.764 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
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151,715 - GFF3146 0.14 +1.0
151,816 + GFF3146 0.26 +0.7
152,436 + +1.5
152,568 + GFF3147 0.28 +0.1
152,664 + GFF3147 0.51 -0.3
152,664 + GFF3147 0.51 +0.0
152,704 + GFF3147 0.60 -0.6
152,744 + GFF3147 0.70 +0.2
153,007 - GFF3148 0.22 -0.3
153,037 - GFF3148 0.27 -0.3
153,076 - GFF3148 0.33 -0.1
153,076 - GFF3148 0.33 -0.2
153,113 + GFF3148 0.39 -0.2
153,205 + GFF3148 0.53 +0.4
153,522 - +1.0
153,529 - +0.0
153,529 - +0.7
153,603 - +0.2
153,684 - -2.1
153,763 + -1.5
153,764 - +0.0

Or see this region's nucleotide sequence