Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3120 and GFF3121 are separated by 139 nucleotides GFF3121 and GFF3122 are separated by 63 nucleotides GFF3122 and GFF3123 overlap by 11 nucleotides
GFF3120 - Cystathionine beta-lyase (EC 4.4.1.8), at 126,130 to 127,317
GFF3120
GFF3121 - DedA family inner membrane protein YghB, at 127,457 to 128,116
GFF3121
GFF3122 - Transcriptional regulator, AraC family, at 128,180 to 129,079
GFF3122
GFF3123 - hypothetical protein, at 129,069 to 129,185
GFF3123
Position (kb) 127
128
129 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 126.460 kb on + strand, within GFF3120 at 126.558 kb on + strand, within GFF3120 at 126.581 kb on + strand, within GFF3120 at 126.687 kb on + strand, within GFF3120 at 126.735 kb on + strand, within GFF3120 at 126.735 kb on + strand, within GFF3120 at 126.877 kb on + strand, within GFF3120 at 126.878 kb on - strand, within GFF3120 at 126.878 kb on - strand, within GFF3120 at 126.878 kb on - strand, within GFF3120 at 126.879 kb on + strand, within GFF3120 at 126.880 kb on - strand, within GFF3120 at 126.880 kb on - strand, within GFF3120 at 126.880 kb on - strand, within GFF3120 at 127.009 kb on - strand, within GFF3120 at 127.108 kb on + strand, within GFF3120 at 127.142 kb on - strand, within GFF3120 at 127.176 kb on + strand, within GFF3120 at 127.316 kb on - strand at 127.336 kb on + strand at 127.337 kb on - strand at 127.337 kb on - strand at 127.338 kb on + strand at 127.338 kb on + strand at 127.338 kb on + strand at 127.338 kb on + strand at 127.339 kb on - strand at 127.339 kb on - strand at 127.339 kb on - strand at 127.339 kb on - strand at 127.339 kb on - strand at 127.339 kb on - strand at 127.412 kb on - strand at 127.417 kb on - strand at 127.432 kb on + strand at 127.475 kb on - strand at 127.542 kb on - strand, within GFF3121 at 127.599 kb on + strand, within GFF3121 at 127.600 kb on - strand, within GFF3121 at 127.715 kb on + strand, within GFF3121 at 127.739 kb on - strand, within GFF3121 at 127.741 kb on + strand, within GFF3121 at 127.742 kb on - strand, within GFF3121 at 127.785 kb on - strand, within GFF3121 at 127.827 kb on + strand, within GFF3121 at 127.827 kb on + strand, within GFF3121 at 128.019 kb on + strand, within GFF3121 at 128.020 kb on - strand, within GFF3121 at 128.052 kb on + strand at 128.108 kb on - strand at 128.120 kb on - strand at 128.120 kb on - strand at 128.182 kb on - strand at 128.182 kb on - strand at 128.246 kb on - strand at 128.246 kb on - strand at 128.470 kb on - strand, within GFF3122 at 128.488 kb on + strand, within GFF3122 at 128.850 kb on - strand, within GFF3122 at 128.976 kb on + strand, within GFF3122 at 129.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 126,460 + GFF3120 0.28 -0.2 126,558 + GFF3120 0.36 +0.2 126,581 + GFF3120 0.38 -0.1 126,687 + GFF3120 0.47 -0.1 126,735 + GFF3120 0.51 -0.7 126,735 + GFF3120 0.51 +0.3 126,877 + GFF3120 0.63 +0.9 126,878 - GFF3120 0.63 +0.1 126,878 - GFF3120 0.63 -0.2 126,878 - GFF3120 0.63 +0.2 126,879 + GFF3120 0.63 +0.4 126,880 - GFF3120 0.63 -0.1 126,880 - GFF3120 0.63 +0.1 126,880 - GFF3120 0.63 +0.1 127,009 - GFF3120 0.74 +1.7 127,108 + GFF3120 0.82 -0.1 127,142 - GFF3120 0.85 +0.1 127,176 + GFF3120 0.88 +0.0 127,316 - +0.4 127,336 + -0.1 127,337 - +0.4 127,337 - +0.4 127,338 + +0.2 127,338 + -0.1 127,338 + +0.3 127,338 + +0.4 127,339 - -0.1 127,339 - +1.1 127,339 - -0.3 127,339 - -0.2 127,339 - -0.0 127,339 - +0.5 127,412 - +0.1 127,417 - -0.4 127,432 + +0.1 127,475 - -0.1 127,542 - GFF3121 0.13 -2.8 127,599 + GFF3121 0.22 +0.2 127,600 - GFF3121 0.22 -0.2 127,715 + GFF3121 0.39 +1.3 127,739 - GFF3121 0.43 +0.2 127,741 + GFF3121 0.43 +0.5 127,742 - GFF3121 0.43 +0.7 127,785 - GFF3121 0.50 +1.0 127,827 + GFF3121 0.56 +0.2 127,827 + GFF3121 0.56 +0.3 128,019 + GFF3121 0.85 +0.5 128,020 - GFF3121 0.85 +0.1 128,052 + +0.3 128,108 - -0.2 128,120 - +0.0 128,120 - +0.6 128,182 - -0.0 128,182 - -0.2 128,246 - -0.2 128,246 - +0.2 128,470 - GFF3122 0.32 +0.1 128,488 + GFF3122 0.34 +1.4 128,850 - GFF3122 0.74 +0.3 128,976 + GFF3122 0.88 +0.6 129,012 - +0.4
Or see this region's nucleotide sequence