Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3074 and GFF3075 are separated by 23 nucleotides GFF3075 and GFF3076 are separated by 91 nucleotides GFF3076 and GFF3077 are separated by 47 nucleotides
GFF3074 - Acyl dehydratase, at 83,310 to 83,837
GFF3074
GFF3075 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 83,861 to 84,703
GFF3075
GFF3076 - LysR family transcriptional regulator STM3121, at 84,795 to 85,673
GFF3076
GFF3077 - Arylsulfatase (EC 3.1.6.1), at 85,721 to 87,460
GFF3077
Position (kb) 84
85
86 Strain fitness (log2 ratio) -1
0
1
2 at 83.922 kb on - strand at 84.023 kb on + strand, within GFF3075 at 84.322 kb on + strand, within GFF3075 at 84.362 kb on - strand, within GFF3075 at 84.418 kb on - strand, within GFF3075 at 84.448 kb on + strand, within GFF3075 at 84.448 kb on + strand, within GFF3075 at 84.449 kb on - strand, within GFF3075 at 84.449 kb on - strand, within GFF3075 at 84.494 kb on + strand, within GFF3075 at 84.674 kb on - strand at 84.674 kb on - strand at 84.757 kb on + strand at 84.758 kb on - strand at 84.874 kb on + strand at 84.874 kb on + strand at 84.875 kb on - strand at 84.902 kb on + strand, within GFF3076 at 84.999 kb on - strand, within GFF3076 at 85.086 kb on - strand, within GFF3076 at 85.181 kb on + strand, within GFF3076 at 85.298 kb on + strand, within GFF3076 at 85.299 kb on - strand, within GFF3076 at 85.562 kb on - strand, within GFF3076 at 85.565 kb on - strand, within GFF3076 at 85.697 kb on - strand at 85.719 kb on - strand at 85.830 kb on + strand at 85.830 kb on + strand at 86.004 kb on - strand, within GFF3077 at 86.057 kb on - strand, within GFF3077 at 86.074 kb on - strand, within GFF3077 at 86.238 kb on - strand, within GFF3077 at 86.258 kb on - strand, within GFF3077 at 86.288 kb on - strand, within GFF3077 at 86.372 kb on - strand, within GFF3077 at 86.480 kb on + strand, within GFF3077 at 86.481 kb on - strand, within GFF3077 at 86.570 kb on - strand, within GFF3077 at 86.570 kb on - strand, within GFF3077 at 86.602 kb on - strand, within GFF3077
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 83,922 - +0.3 84,023 + GFF3075 0.19 -0.1 84,322 + GFF3075 0.55 +0.1 84,362 - GFF3075 0.59 -0.3 84,418 - GFF3075 0.66 -0.0 84,448 + GFF3075 0.70 -0.4 84,448 + GFF3075 0.70 +0.3 84,449 - GFF3075 0.70 +0.9 84,449 - GFF3075 0.70 +0.4 84,494 + GFF3075 0.75 -0.1 84,674 - +0.2 84,674 - +0.6 84,757 + -0.0 84,758 - -0.6 84,874 + +0.1 84,874 + +0.1 84,875 - +0.2 84,902 + GFF3076 0.12 -0.1 84,999 - GFF3076 0.23 -0.8 85,086 - GFF3076 0.33 -0.8 85,181 + GFF3076 0.44 +0.1 85,298 + GFF3076 0.57 +0.2 85,299 - GFF3076 0.57 +0.3 85,562 - GFF3076 0.87 -0.3 85,565 - GFF3076 0.88 -0.2 85,697 - -0.6 85,719 - +0.0 85,830 + +0.4 85,830 + +1.9 86,004 - GFF3077 0.16 +0.9 86,057 - GFF3077 0.19 -0.2 86,074 - GFF3077 0.20 -0.4 86,238 - GFF3077 0.30 +0.1 86,258 - GFF3077 0.31 -0.0 86,288 - GFF3077 0.33 +0.1 86,372 - GFF3077 0.37 +0.1 86,480 + GFF3077 0.44 +0.0 86,481 - GFF3077 0.44 -0.2 86,570 - GFF3077 0.49 +0.1 86,570 - GFF3077 0.49 +0.3 86,602 - GFF3077 0.51 +0.1
Or see this region's nucleotide sequence