Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF306 and GFF307 are separated by 142 nucleotides GFF307 and GFF308 are separated by 192 nucleotides GFF308 and GFF309 are separated by 352 nucleotides
GFF306 - Hexose phosphate uptake regulatory protein UhpC, at 24,036 to 25,364
GFF306
GFF307 - Hexose phosphate transport protein UhpT, at 25,507 to 26,898
GFF307
GFF308 - probable membrane protein YPO2654, at 27,091 to 27,543
GFF308
GFF309 - Putative transcriptional regulator of unknown carbohydrate utilization cluster, GntR family, at 27,896 to 28,639
GFF309
Position (kb) 25
26
27 Strain fitness (log2 ratio) -2
-1
0
1
2 at 24.555 kb on - strand, within GFF306 at 24.786 kb on + strand, within GFF306 at 24.862 kb on - strand, within GFF306 at 24.863 kb on + strand, within GFF306 at 24.863 kb on + strand, within GFF306 at 24.910 kb on + strand, within GFF306 at 24.910 kb on + strand, within GFF306 at 25.066 kb on - strand, within GFF306 at 25.066 kb on - strand, within GFF306 at 25.066 kb on - strand, within GFF306 at 25.158 kb on - strand, within GFF306 at 25.365 kb on + strand at 25.365 kb on + strand at 25.366 kb on - strand at 25.520 kb on + strand at 25.570 kb on - strand at 25.601 kb on - strand at 25.634 kb on - strand at 25.634 kb on - strand at 25.680 kb on + strand, within GFF307 at 25.739 kb on - strand, within GFF307 at 25.765 kb on + strand, within GFF307 at 25.806 kb on + strand, within GFF307 at 25.830 kb on + strand, within GFF307 at 25.830 kb on + strand, within GFF307 at 25.831 kb on - strand, within GFF307 at 25.894 kb on + strand, within GFF307 at 25.895 kb on - strand, within GFF307 at 26.001 kb on + strand, within GFF307 at 26.059 kb on + strand, within GFF307 at 26.060 kb on - strand, within GFF307 at 26.164 kb on + strand, within GFF307 at 26.328 kb on - strand, within GFF307 at 26.386 kb on - strand, within GFF307 at 26.410 kb on - strand, within GFF307 at 26.410 kb on - strand, within GFF307 at 26.557 kb on + strand, within GFF307 at 26.557 kb on + strand, within GFF307 at 26.557 kb on + strand, within GFF307 at 26.558 kb on - strand, within GFF307 at 26.558 kb on - strand, within GFF307 at 26.558 kb on - strand, within GFF307 at 26.558 kb on - strand, within GFF307 at 26.693 kb on - strand, within GFF307 at 26.814 kb on + strand at 26.946 kb on + strand at 27.007 kb on + strand at 27.129 kb on + strand at 27.198 kb on + strand, within GFF308 at 27.317 kb on + strand, within GFF308 at 27.418 kb on - strand, within GFF308 at 27.425 kb on + strand, within GFF308 at 27.498 kb on + strand at 27.544 kb on + strand at 27.555 kb on - strand at 27.646 kb on - strand at 27.664 kb on + strand at 27.690 kb on + strand at 27.691 kb on - strand at 27.735 kb on - strand at 27.735 kb on - strand at 27.735 kb on - strand at 27.757 kb on + strand at 27.758 kb on - strand at 27.760 kb on - strand at 27.812 kb on - strand at 27.865 kb on - strand at 27.867 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 24,555 - GFF306 0.39 +0.2 24,786 + GFF306 0.56 -0.8 24,862 - GFF306 0.62 +0.3 24,863 + GFF306 0.62 -0.2 24,863 + GFF306 0.62 +0.2 24,910 + GFF306 0.66 +0.1 24,910 + GFF306 0.66 +0.1 25,066 - GFF306 0.78 +0.3 25,066 - GFF306 0.78 -0.4 25,066 - GFF306 0.78 +0.1 25,158 - GFF306 0.84 +0.2 25,365 + -0.7 25,365 + +0.4 25,366 - -0.1 25,520 + -0.8 25,570 - +0.2 25,601 - +0.4 25,634 - +0.2 25,634 - -0.1 25,680 + GFF307 0.12 -0.7 25,739 - GFF307 0.17 +0.3 25,765 + GFF307 0.19 -0.8 25,806 + GFF307 0.21 +0.2 25,830 + GFF307 0.23 +0.0 25,830 + GFF307 0.23 -0.8 25,831 - GFF307 0.23 -0.9 25,894 + GFF307 0.28 -0.9 25,895 - GFF307 0.28 -0.6 26,001 + GFF307 0.35 -0.1 26,059 + GFF307 0.40 +0.4 26,060 - GFF307 0.40 -0.3 26,164 + GFF307 0.47 +0.2 26,328 - GFF307 0.59 -0.1 26,386 - GFF307 0.63 -0.1 26,410 - GFF307 0.65 -0.0 26,410 - GFF307 0.65 +0.7 26,557 + GFF307 0.75 -0.4 26,557 + GFF307 0.75 -0.2 26,557 + GFF307 0.75 +0.2 26,558 - GFF307 0.76 -0.0 26,558 - GFF307 0.76 -0.4 26,558 - GFF307 0.76 +1.1 26,558 - GFF307 0.76 -0.1 26,693 - GFF307 0.85 -0.1 26,814 + +0.0 26,946 + -0.3 27,007 + -0.3 27,129 + +0.2 27,198 + GFF308 0.24 +0.4 27,317 + GFF308 0.50 -0.9 27,418 - GFF308 0.72 +0.2 27,425 + GFF308 0.74 +0.3 27,498 + +0.1 27,544 + +0.1 27,555 - -0.4 27,646 - +0.5 27,664 + +1.9 27,690 + +0.2 27,691 - +0.5 27,735 - +0.0 27,735 - -1.2 27,735 - -0.2 27,757 + +0.1 27,758 - +0.2 27,760 - -0.1 27,812 - -1.9 27,865 - +0.1 27,867 - -0.2
Or see this region's nucleotide sequence