Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2962 and GFF2963 overlap by 4 nucleotides GFF2963 and GFF2964 are separated by 46 nucleotides GFF2964 and GFF2965 are separated by 115 nucleotides
GFF2962 - N-acetylmannosamine kinase (EC 2.7.1.60), at 46,381 to 47,256
GFF2962
GFF2963 - N-acetylmannosamine-6-phosphate 2-epimerase (EC 5.1.3.9), at 47,253 to 47,942
GFF2963
GFF2964 - Sialic acid transporter (permease) NanT, at 47,989 to 49,479
GFF2964
GFF2965 - N-acetylneuraminate lyase (EC 4.1.3.3), at 49,595 to 50,488
GFF2965
Position (kb) 47
48
49
50 Strain fitness (log2 ratio) -1
0
1 at 47.074 kb on + strand, within GFF2962 at 47.224 kb on - strand at 47.250 kb on + strand at 47.251 kb on - strand at 47.251 kb on - strand at 47.382 kb on - strand, within GFF2963 at 47.382 kb on - strand, within GFF2963 at 47.383 kb on + strand, within GFF2963 at 47.474 kb on - strand, within GFF2963 at 47.671 kb on - strand, within GFF2963 at 47.779 kb on - strand, within GFF2963 at 47.789 kb on + strand, within GFF2963 at 47.790 kb on - strand, within GFF2963 at 47.912 kb on + strand at 47.934 kb on + strand at 47.935 kb on - strand at 47.943 kb on - strand at 47.943 kb on - strand at 47.946 kb on - strand at 47.946 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 48.106 kb on + strand at 48.106 kb on + strand at 48.120 kb on + strand at 48.132 kb on + strand at 48.256 kb on - strand, within GFF2964 at 48.257 kb on + strand, within GFF2964 at 48.257 kb on + strand, within GFF2964 at 48.262 kb on - strand, within GFF2964 at 48.300 kb on - strand, within GFF2964 at 48.450 kb on - strand, within GFF2964 at 48.450 kb on - strand, within GFF2964 at 48.578 kb on + strand, within GFF2964 at 48.579 kb on - strand, within GFF2964 at 48.587 kb on - strand, within GFF2964 at 48.589 kb on + strand, within GFF2964 at 48.612 kb on - strand, within GFF2964 at 48.712 kb on + strand, within GFF2964 at 48.846 kb on + strand, within GFF2964 at 48.847 kb on - strand, within GFF2964 at 48.847 kb on - strand, within GFF2964 at 49.085 kb on + strand, within GFF2964 at 49.086 kb on - strand, within GFF2964 at 49.474 kb on - strand at 49.482 kb on + strand at 49.589 kb on - strand at 49.676 kb on - strand at 49.676 kb on - strand at 49.706 kb on - strand, within GFF2965 at 49.818 kb on - strand, within GFF2965 at 49.860 kb on + strand, within GFF2965 at 49.861 kb on - strand, within GFF2965 at 49.861 kb on - strand, within GFF2965 at 49.930 kb on - strand, within GFF2965 at 49.930 kb on - strand, within GFF2965 at 50.030 kb on - strand, within GFF2965 at 50.054 kb on + strand, within GFF2965 at 50.079 kb on + strand, within GFF2965 at 50.079 kb on + strand, within GFF2965 at 50.080 kb on - strand, within GFF2965 at 50.160 kb on + strand, within GFF2965 at 50.161 kb on - strand, within GFF2965 at 50.197 kb on - strand, within GFF2965 at 50.361 kb on + strand, within GFF2965 at 50.362 kb on - strand, within GFF2965
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 47,074 + GFF2962 0.79 -0.4 47,224 - +0.5 47,250 + +0.8 47,251 - +0.1 47,251 - +0.2 47,382 - GFF2963 0.19 -0.5 47,382 - GFF2963 0.19 +0.4 47,383 + GFF2963 0.19 -0.2 47,474 - GFF2963 0.32 +0.4 47,671 - GFF2963 0.61 -0.5 47,779 - GFF2963 0.76 +0.3 47,789 + GFF2963 0.78 -0.4 47,790 - GFF2963 0.78 +0.0 47,912 + -0.2 47,934 + +0.7 47,935 - +0.4 47,943 - +0.2 47,943 - +0.4 47,946 - +0.2 47,946 - +0.4 47,960 - +0.0 47,960 - -0.2 48,106 + -0.1 48,106 + -0.3 48,120 + +0.5 48,132 + +0.5 48,256 - GFF2964 0.18 +0.1 48,257 + GFF2964 0.18 +0.3 48,257 + GFF2964 0.18 -1.0 48,262 - GFF2964 0.18 +0.1 48,300 - GFF2964 0.21 +1.4 48,450 - GFF2964 0.31 -0.0 48,450 - GFF2964 0.31 +0.1 48,578 + GFF2964 0.40 -0.3 48,579 - GFF2964 0.40 +0.0 48,587 - GFF2964 0.40 +0.2 48,589 + GFF2964 0.40 -0.1 48,612 - GFF2964 0.42 -0.1 48,712 + GFF2964 0.48 +0.2 48,846 + GFF2964 0.57 +0.6 48,847 - GFF2964 0.58 -0.1 48,847 - GFF2964 0.58 +0.8 49,085 + GFF2964 0.74 -0.9 49,086 - GFF2964 0.74 -0.3 49,474 - +0.1 49,482 + -0.1 49,589 - -0.4 49,676 - -1.3 49,676 - +0.2 49,706 - GFF2965 0.12 -0.2 49,818 - GFF2965 0.25 +0.0 49,860 + GFF2965 0.30 -0.7 49,861 - GFF2965 0.30 +0.9 49,861 - GFF2965 0.30 +0.3 49,930 - GFF2965 0.37 +0.7 49,930 - GFF2965 0.37 +0.8 50,030 - GFF2965 0.49 +0.5 50,054 + GFF2965 0.51 +0.1 50,079 + GFF2965 0.54 +0.2 50,079 + GFF2965 0.54 +0.1 50,080 - GFF2965 0.54 +1.3 50,160 + GFF2965 0.63 -0.1 50,161 - GFF2965 0.63 +0.2 50,197 - GFF2965 0.67 +0.5 50,361 + GFF2965 0.86 +0.8 50,362 - GFF2965 0.86 -0.2
Or see this region's nucleotide sequence