Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2687 and GFF2688 are separated by 225 nucleotides GFF2688 and GFF2689 overlap by 4 nucleotides GFF2689 and GFF2690 overlap by 14 nucleotides
GFF2687 - Psp operon transcriptional activator, at 208,189 to 209,184
GFF2687
GFF2688 - Peptide transport periplasmic protein SapA, at 209,410 to 210,945
GFF2688
GFF2689 - Peptide transport system permease protein SapB, at 210,942 to 211,907
GFF2689
GFF2690 - Peptide transport system permease protein SapC, at 211,894 to 212,784
GFF2690
Position (kb) 209
210
211 Strain fitness (log2 ratio) -2
-1
0
1 at 208.579 kb on + strand, within GFF2687 at 208.634 kb on + strand, within GFF2687 at 208.725 kb on - strand, within GFF2687 at 208.805 kb on + strand, within GFF2687 at 208.805 kb on + strand, within GFF2687 at 208.805 kb on + strand, within GFF2687 at 208.806 kb on - strand, within GFF2687 at 208.862 kb on - strand, within GFF2687 at 208.875 kb on + strand, within GFF2687 at 209.143 kb on + strand at 209.344 kb on - strand at 209.442 kb on + strand at 209.527 kb on - strand at 209.530 kb on + strand at 209.731 kb on - strand, within GFF2688 at 209.748 kb on + strand, within GFF2688 at 209.753 kb on + strand, within GFF2688 at 209.753 kb on + strand, within GFF2688 at 209.860 kb on + strand, within GFF2688 at 209.860 kb on + strand, within GFF2688 at 210.376 kb on + strand, within GFF2688 at 210.702 kb on + strand, within GFF2688 at 210.833 kb on + strand at 210.876 kb on - strand at 211.487 kb on + strand, within GFF2689 at 211.559 kb on + strand, within GFF2689 at 211.581 kb on + strand, within GFF2689 at 211.782 kb on + strand, within GFF2689
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 208,579 + GFF2687 0.39 -0.3 208,634 + GFF2687 0.45 +0.0 208,725 - GFF2687 0.54 +0.8 208,805 + GFF2687 0.62 +0.3 208,805 + GFF2687 0.62 -0.1 208,805 + GFF2687 0.62 +0.0 208,806 - GFF2687 0.62 -1.2 208,862 - GFF2687 0.68 -1.3 208,875 + GFF2687 0.69 +0.2 209,143 + +1.3 209,344 - -2.5 209,442 + -2.7 209,527 - -2.5 209,530 + -1.6 209,731 - GFF2688 0.21 -1.1 209,748 + GFF2688 0.22 -2.5 209,753 + GFF2688 0.22 -1.4 209,753 + GFF2688 0.22 -1.5 209,860 + GFF2688 0.29 -0.7 209,860 + GFF2688 0.29 -1.5 210,376 + GFF2688 0.63 -0.7 210,702 + GFF2688 0.84 -1.0 210,833 + -0.6 210,876 - -1.5 211,487 + GFF2689 0.56 -1.2 211,559 + GFF2689 0.64 -0.1 211,581 + GFF2689 0.66 -2.5 211,782 + GFF2689 0.87 -0.7
Or see this region's nucleotide sequence