Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2661 and GFF2662 are separated by 141 nucleotides GFF2662 and GFF2663 are separated by 105 nucleotides GFF2663 and GFF2664 are separated by 137 nucleotides GFF2664 and GFF2665 are separated by 26 nucleotides
GFF2661 - FIG01045442: hypothetical protein, at 184,153 to 184,839
GFF2661
GFF2662 - FIG01045240: hypothetical protein, at 184,981 to 185,205
GFF2662
GFF2663 - Thiol peroxidase, Tpx-type (EC 1.11.1.15), at 185,311 to 185,829
GFF2663
GFF2664 - FIG01200701: possible membrane protein, at 185,967 to 186,791
GFF2664
GFF2665 - Invasin-like protein, at 186,818 to 188,800
GFF2665
Position (kb) 185
186 Strain fitness (log2 ratio) -1
0
1 at 184.331 kb on + strand, within GFF2661 at 184.332 kb on - strand, within GFF2661 at 184.463 kb on - strand, within GFF2661 at 184.463 kb on - strand, within GFF2661 at 184.465 kb on - strand, within GFF2661 at 184.483 kb on + strand, within GFF2661 at 184.519 kb on + strand, within GFF2661 at 184.570 kb on - strand, within GFF2661 at 184.620 kb on + strand, within GFF2661 at 184.677 kb on - strand, within GFF2661 at 184.702 kb on - strand, within GFF2661 at 184.702 kb on - strand, within GFF2661 at 184.756 kb on + strand, within GFF2661 at 185.215 kb on - strand at 185.334 kb on + strand at 185.350 kb on - strand at 185.384 kb on - strand, within GFF2663 at 185.543 kb on + strand, within GFF2663 at 186.169 kb on - strand, within GFF2664 at 186.448 kb on - strand, within GFF2664 at 186.767 kb on - strand at 186.792 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 184,331 + GFF2661 0.26 +0.0 184,332 - GFF2661 0.26 -0.8 184,463 - GFF2661 0.45 +0.2 184,463 - GFF2661 0.45 +0.1 184,465 - GFF2661 0.45 +0.2 184,483 + GFF2661 0.48 -0.6 184,519 + GFF2661 0.53 +0.1 184,570 - GFF2661 0.61 +0.5 184,620 + GFF2661 0.68 +0.5 184,677 - GFF2661 0.76 +0.3 184,702 - GFF2661 0.80 -0.1 184,702 - GFF2661 0.80 +0.1 184,756 + GFF2661 0.88 +0.3 185,215 - +1.3 185,334 + +0.5 185,350 - -1.7 185,384 - GFF2663 0.14 -0.8 185,543 + GFF2663 0.45 -0.4 186,169 - GFF2664 0.24 -0.1 186,448 - GFF2664 0.58 +0.1 186,767 - +0.7 186,792 - -0.4
Or see this region's nucleotide sequence