Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2434

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2432 and GFF2433 are separated by 195 nucleotidesGFF2433 and GFF2434 are separated by 28 nucleotidesGFF2434 and GFF2435 are separated by 27 nucleotides GFF2432 - Chaperone protein HscB, at 48,544 to 49,059 GFF2432 GFF2433 - Iron binding protein IscA for iron-sulfur cluster assembly, at 49,255 to 49,578 GFF2433 GFF2434 - Iron-sulfur cluster assembly scaffold protein IscU, at 49,607 to 49,993 GFF2434 GFF2435 - Cysteine desulfurase (EC 2.8.1.7), IscS subfamily, at 50,021 to 51,235 GFF2435 Position (kb) 49 50Strain fitness (log2 ratio) -3 -2 -1 0 1at 48.808 kb on - strand, within GFF2432at 48.808 kb on - strand, within GFF2432at 49.121 kb on + strandat 49.122 kb on - strandat 49.237 kb on + strandat 49.257 kb on - strandat 49.460 kb on + strand, within GFF2433at 49.460 kb on + strand, within GFF2433at 49.460 kb on + strand, within GFF2433at 49.461 kb on - strand, within GFF2433at 49.461 kb on - strand, within GFF2433at 49.461 kb on - strand, within GFF2433at 49.461 kb on - strand, within GFF2433at 49.461 kb on - strand, within GFF2433at 49.579 kb on - strandat 49.633 kb on - strandat 49.919 kb on - strand, within GFF2434at 50.068 kb on - strandat 50.113 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
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48,808 - GFF2432 0.51 -1.7
48,808 - GFF2432 0.51 -0.6
49,121 + -3.6
49,122 - -0.4
49,237 + -0.4
49,257 - -0.2
49,460 + GFF2433 0.63 -1.9
49,460 + GFF2433 0.63 -1.9
49,460 + GFF2433 0.63 -2.2
49,461 - GFF2433 0.64 -0.8
49,461 - GFF2433 0.64 -0.9
49,461 - GFF2433 0.64 -1.1
49,461 - GFF2433 0.64 -2.4
49,461 - GFF2433 0.64 -0.6
49,579 - -0.0
49,633 - -1.4
49,919 - GFF2434 0.81 -1.9
50,068 - -0.5
50,113 + -1.0

Or see this region's nucleotide sequence