Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2338 and GFF2339 are separated by 1 nucleotides GFF2339 and GFF2340 overlap by 14 nucleotides GFF2340 and GFF2341 are separated by 160 nucleotides
GFF2338 - Pyruvate formate-lyase activating enzyme (EC 1.97.1.4), at 130,001 to 130,879
GFF2338
GFF2339 - PTS system fructose-like IIB component 2 precursor (EC 2.7.1.69), at 130,881 to 131,234
GFF2339
GFF2340 - Transcriptional regulator, AraC family, at 131,221 to 132,072
GFF2340
GFF2341 - UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide, at 132,233 to 133,966
GFF2341
Position (kb) 131
132
133 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 130.292 kb on - strand, within GFF2338 at 130.295 kb on - strand, within GFF2338 at 130.416 kb on + strand, within GFF2338 at 130.485 kb on + strand, within GFF2338 at 130.485 kb on + strand, within GFF2338 at 130.515 kb on + strand, within GFF2338 at 130.515 kb on + strand, within GFF2338 at 130.516 kb on - strand, within GFF2338 at 130.596 kb on + strand, within GFF2338 at 130.707 kb on + strand, within GFF2338 at 130.708 kb on - strand, within GFF2338 at 130.713 kb on + strand, within GFF2338 at 130.944 kb on + strand, within GFF2339 at 130.944 kb on + strand, within GFF2339 at 130.944 kb on + strand, within GFF2339 at 130.944 kb on + strand, within GFF2339 at 130.945 kb on - strand, within GFF2339 at 130.945 kb on - strand, within GFF2339 at 130.945 kb on - strand, within GFF2339 at 130.945 kb on - strand, within GFF2339 at 130.945 kb on - strand, within GFF2339 at 130.946 kb on + strand, within GFF2339 at 130.946 kb on + strand, within GFF2339 at 131.173 kb on + strand, within GFF2339 at 131.214 kb on - strand at 131.295 kb on + strand at 131.296 kb on - strand at 131.322 kb on - strand, within GFF2340 at 131.326 kb on - strand, within GFF2340 at 131.326 kb on - strand, within GFF2340 at 131.326 kb on - strand, within GFF2340 at 131.412 kb on + strand, within GFF2340 at 131.485 kb on - strand, within GFF2340 at 131.487 kb on - strand, within GFF2340 at 131.623 kb on + strand, within GFF2340 at 131.624 kb on - strand, within GFF2340 at 131.627 kb on - strand, within GFF2340 at 131.685 kb on + strand, within GFF2340 at 131.685 kb on + strand, within GFF2340 at 131.792 kb on - strand, within GFF2340 at 132.068 kb on + strand at 132.068 kb on + strand at 132.068 kb on + strand at 132.069 kb on - strand at 132.069 kb on - strand at 132.227 kb on + strand at 132.227 kb on + strand at 132.227 kb on + strand at 132.228 kb on - strand at 132.234 kb on + strand at 132.259 kb on - strand at 132.259 kb on - strand at 132.268 kb on + strand at 132.269 kb on - strand at 132.269 kb on - strand at 132.269 kb on - strand at 132.274 kb on - strand at 132.274 kb on - strand at 132.297 kb on + strand at 132.372 kb on + strand at 132.372 kb on + strand at 132.372 kb on + strand at 132.386 kb on + strand at 132.424 kb on - strand, within GFF2341 at 132.424 kb on - strand, within GFF2341 at 132.505 kb on + strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.508 kb on - strand, within GFF2341 at 132.511 kb on + strand, within GFF2341 at 132.511 kb on + strand, within GFF2341 at 132.512 kb on - strand, within GFF2341 at 132.512 kb on - strand, within GFF2341 at 132.568 kb on - strand, within GFF2341 at 132.568 kb on - strand, within GFF2341 at 132.627 kb on + strand, within GFF2341 at 132.628 kb on - strand, within GFF2341 at 132.654 kb on + strand, within GFF2341 at 132.681 kb on + strand, within GFF2341 at 132.691 kb on - strand, within GFF2341 at 132.713 kb on + strand, within GFF2341 at 132.714 kb on - strand, within GFF2341 at 132.714 kb on - strand, within GFF2341 at 132.780 kb on + strand, within GFF2341 at 132.780 kb on + strand, within GFF2341 at 132.781 kb on - strand, within GFF2341 at 132.912 kb on + strand, within GFF2341 at 133.001 kb on - strand, within GFF2341 at 133.022 kb on - strand, within GFF2341
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 130,292 - GFF2338 0.33 -0.2 130,295 - GFF2338 0.33 -0.1 130,416 + GFF2338 0.47 +0.0 130,485 + GFF2338 0.55 -0.3 130,485 + GFF2338 0.55 +0.3 130,515 + GFF2338 0.58 +0.0 130,515 + GFF2338 0.58 +0.4 130,516 - GFF2338 0.59 -0.6 130,596 + GFF2338 0.68 -0.0 130,707 + GFF2338 0.80 +0.4 130,708 - GFF2338 0.80 +0.0 130,713 + GFF2338 0.81 +0.3 130,944 + GFF2339 0.18 +0.1 130,944 + GFF2339 0.18 -0.0 130,944 + GFF2339 0.18 -1.1 130,944 + GFF2339 0.18 +0.1 130,945 - GFF2339 0.18 +0.1 130,945 - GFF2339 0.18 -0.1 130,945 - GFF2339 0.18 -0.1 130,945 - GFF2339 0.18 -0.1 130,945 - GFF2339 0.18 -0.0 130,946 + GFF2339 0.18 +0.5 130,946 + GFF2339 0.18 -0.0 131,173 + GFF2339 0.82 -2.3 131,214 - -0.5 131,295 + +0.1 131,296 - +0.2 131,322 - GFF2340 0.12 +0.2 131,326 - GFF2340 0.12 +0.3 131,326 - GFF2340 0.12 +0.1 131,326 - GFF2340 0.12 +0.5 131,412 + GFF2340 0.22 +0.3 131,485 - GFF2340 0.31 +0.2 131,487 - GFF2340 0.31 +3.6 131,623 + GFF2340 0.47 +1.0 131,624 - GFF2340 0.47 +0.7 131,627 - GFF2340 0.48 -0.2 131,685 + GFF2340 0.54 +0.4 131,685 + GFF2340 0.54 -0.0 131,792 - GFF2340 0.67 -0.2 132,068 + -1.2 132,068 + +0.3 132,068 + +0.1 132,069 - +0.1 132,069 - +0.2 132,227 + +0.2 132,227 + -0.2 132,227 + -0.1 132,228 - +0.2 132,234 + -0.3 132,259 - +2.0 132,259 - +0.8 132,268 + -0.1 132,269 - +0.1 132,269 - +0.2 132,269 - +0.4 132,274 - +0.4 132,274 - -0.1 132,297 + -0.7 132,372 + +0.7 132,372 + +0.0 132,372 + -0.1 132,386 + +0.2 132,424 - GFF2341 0.11 +0.1 132,424 - GFF2341 0.11 -0.1 132,505 + GFF2341 0.16 -0.6 132,508 - GFF2341 0.16 +0.1 132,508 - GFF2341 0.16 +0.3 132,508 - GFF2341 0.16 +0.2 132,508 - GFF2341 0.16 -0.4 132,508 - GFF2341 0.16 +0.7 132,508 - GFF2341 0.16 +0.4 132,508 - GFF2341 0.16 +0.0 132,508 - GFF2341 0.16 +0.9 132,511 + GFF2341 0.16 +0.6 132,511 + GFF2341 0.16 +0.3 132,512 - GFF2341 0.16 +0.1 132,512 - GFF2341 0.16 +0.1 132,568 - GFF2341 0.19 +0.6 132,568 - GFF2341 0.19 +0.4 132,627 + GFF2341 0.23 +0.5 132,628 - GFF2341 0.23 +3.3 132,654 + GFF2341 0.24 +0.2 132,681 + GFF2341 0.26 +0.2 132,691 - GFF2341 0.26 +1.1 132,713 + GFF2341 0.28 +0.2 132,714 - GFF2341 0.28 +0.0 132,714 - GFF2341 0.28 -0.2 132,780 + GFF2341 0.32 -0.1 132,780 + GFF2341 0.32 -0.5 132,781 - GFF2341 0.32 +1.0 132,912 + GFF2341 0.39 -0.3 133,001 - GFF2341 0.44 +0.4 133,022 - GFF2341 0.46 +0.2
Or see this region's nucleotide sequence