Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2221 and GFF2222 are separated by 274 nucleotides GFF2222 and GFF2223 are separated by 375 nucleotides
GFF2221 - Nitrogen regulation protein NtrB (EC 2.7.13.3), at 13,199 to 14,248
GFF2221
GFF2222 - Glutamine synthetase type I (EC 6.3.1.2), at 14,523 to 15,932
GFF2222
GFF2223 - GTP-binding protein TypA/BipA, at 16,308 to 18,131
GFF2223
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1 at 13.526 kb on + strand, within GFF2221 at 13.579 kb on - strand, within GFF2221 at 13.649 kb on - strand, within GFF2221 at 13.652 kb on + strand, within GFF2221 at 13.652 kb on + strand, within GFF2221 at 13.652 kb on + strand, within GFF2221 at 13.653 kb on - strand, within GFF2221 at 13.715 kb on + strand, within GFF2221 at 13.716 kb on - strand, within GFF2221 at 13.750 kb on - strand, within GFF2221 at 13.787 kb on + strand, within GFF2221 at 13.937 kb on + strand, within GFF2221 at 13.938 kb on - strand, within GFF2221 at 13.939 kb on + strand, within GFF2221 at 13.939 kb on + strand, within GFF2221 at 14.300 kb on - strand at 14.453 kb on + strand at 14.453 kb on + strand at 14.454 kb on - strand at 14.454 kb on - strand at 14.454 kb on - strand at 14.454 kb on - strand at 14.454 kb on - strand at 14.533 kb on + strand at 14.567 kb on + strand at 14.568 kb on - strand at 14.605 kb on + strand at 14.605 kb on + strand at 15.041 kb on - strand, within GFF2222 at 15.044 kb on - strand, within GFF2222 at 15.044 kb on - strand, within GFF2222 at 15.212 kb on - strand, within GFF2222 at 15.212 kb on - strand, within GFF2222 at 15.446 kb on + strand, within GFF2222 at 15.447 kb on - strand, within GFF2222 at 15.501 kb on - strand, within GFF2222 at 15.632 kb on - strand, within GFF2222 at 15.933 kb on - strand at 15.939 kb on - strand at 15.939 kb on - strand at 15.939 kb on - strand at 16.015 kb on - strand at 16.178 kb on + strand at 16.359 kb on - strand at 16.377 kb on - strand at 16.470 kb on - strand at 16.470 kb on - strand at 16.470 kb on - strand at 16.529 kb on + strand, within GFF2223 at 16.865 kb on - strand, within GFF2223
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 13,526 + GFF2221 0.31 -0.8 13,579 - GFF2221 0.36 +0.5 13,649 - GFF2221 0.43 -0.5 13,652 + GFF2221 0.43 -1.5 13,652 + GFF2221 0.43 -1.7 13,652 + GFF2221 0.43 +0.3 13,653 - GFF2221 0.43 -0.6 13,715 + GFF2221 0.49 +0.9 13,716 - GFF2221 0.49 -0.8 13,750 - GFF2221 0.52 -1.1 13,787 + GFF2221 0.56 -1.0 13,937 + GFF2221 0.70 -1.3 13,938 - GFF2221 0.70 -1.7 13,939 + GFF2221 0.70 -2.4 13,939 + GFF2221 0.70 -1.2 14,300 - +0.4 14,453 + -0.3 14,453 + -1.0 14,454 - -0.6 14,454 - -0.1 14,454 - -0.6 14,454 - +0.2 14,454 - -0.1 14,533 + +0.0 14,567 + -1.1 14,568 - -0.9 14,605 + +1.3 14,605 + -2.5 15,041 - GFF2222 0.37 -1.7 15,044 - GFF2222 0.37 -0.2 15,044 - GFF2222 0.37 -0.8 15,212 - GFF2222 0.49 -0.6 15,212 - GFF2222 0.49 -0.8 15,446 + GFF2222 0.65 -1.5 15,447 - GFF2222 0.66 -1.5 15,501 - GFF2222 0.69 -0.5 15,632 - GFF2222 0.79 -0.9 15,933 - -1.0 15,939 - -0.9 15,939 - -0.9 15,939 - -1.2 16,015 - -0.1 16,178 + +0.2 16,359 - -1.0 16,377 - -0.9 16,470 - -0.5 16,470 - -0.8 16,470 - +0.3 16,529 + GFF2223 0.12 +0.4 16,865 - GFF2223 0.31 -0.5
Or see this region's nucleotide sequence