Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2220 and GFF2221 are separated by 8 nucleotides GFF2221 and GFF2222 are separated by 274 nucleotides
GFF2220 - Nitrogen regulation protein NR(I), at 11,781 to 13,190
GFF2220
GFF2221 - Nitrogen regulation protein NtrB (EC 2.7.13.3), at 13,199 to 14,248
GFF2221
GFF2222 - Glutamine synthetase type I (EC 6.3.1.2), at 14,523 to 15,932
GFF2222
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1 at 12.406 kb on + strand, within GFF2220 at 12.406 kb on + strand, within GFF2220 at 12.407 kb on - strand, within GFF2220 at 12.639 kb on - strand, within GFF2220 at 12.704 kb on + strand, within GFF2220 at 12.731 kb on + strand, within GFF2220 at 12.731 kb on + strand, within GFF2220 at 12.769 kb on - strand, within GFF2220 at 12.804 kb on - strand, within GFF2220 at 12.804 kb on - strand, within GFF2220 at 12.826 kb on + strand, within GFF2220 at 12.869 kb on + strand, within GFF2220 at 12.869 kb on + strand, within GFF2220 at 12.870 kb on - strand, within GFF2220 at 12.930 kb on + strand, within GFF2220 at 12.950 kb on + strand, within GFF2220 at 12.950 kb on + strand, within GFF2220 at 12.983 kb on + strand, within GFF2220 at 12.984 kb on - strand, within GFF2220 at 13.101 kb on - strand at 13.154 kb on + strand at 13.154 kb on + strand at 13.154 kb on + strand at 13.154 kb on + strand at 13.155 kb on - strand at 13.155 kb on - strand at 13.155 kb on - strand at 13.191 kb on - strand at 13.191 kb on - strand at 13.191 kb on - strand at 13.298 kb on - strand at 13.298 kb on - strand at 13.301 kb on + strand at 13.403 kb on - strand, within GFF2221 at 13.516 kb on + strand, within GFF2221 at 13.516 kb on + strand, within GFF2221 at 13.516 kb on + strand, within GFF2221 at 13.516 kb on + strand, within GFF2221 at 13.517 kb on - strand, within GFF2221 at 13.526 kb on + strand, within GFF2221 at 13.579 kb on - strand, within GFF2221 at 13.649 kb on - strand, within GFF2221 at 13.652 kb on + strand, within GFF2221 at 13.652 kb on + strand, within GFF2221 at 13.652 kb on + strand, within GFF2221 at 13.653 kb on - strand, within GFF2221 at 13.715 kb on + strand, within GFF2221 at 13.716 kb on - strand, within GFF2221 at 13.750 kb on - strand, within GFF2221 at 13.787 kb on + strand, within GFF2221 at 13.937 kb on + strand, within GFF2221 at 13.938 kb on - strand, within GFF2221 at 13.939 kb on + strand, within GFF2221 at 13.939 kb on + strand, within GFF2221 at 14.300 kb on - strand at 14.453 kb on + strand at 14.453 kb on + strand at 14.454 kb on - strand at 14.454 kb on - strand at 14.454 kb on - strand at 14.454 kb on - strand at 14.454 kb on - strand at 14.533 kb on + strand at 14.567 kb on + strand at 14.568 kb on - strand at 14.605 kb on + strand at 14.605 kb on + strand at 15.041 kb on - strand, within GFF2222 at 15.044 kb on - strand, within GFF2222 at 15.044 kb on - strand, within GFF2222 at 15.212 kb on - strand, within GFF2222 at 15.212 kb on - strand, within GFF2222
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 12,406 + GFF2220 0.44 -0.6 12,406 + GFF2220 0.44 -1.0 12,407 - GFF2220 0.44 -2.3 12,639 - GFF2220 0.61 -1.2 12,704 + GFF2220 0.65 -0.7 12,731 + GFF2220 0.67 -1.0 12,731 + GFF2220 0.67 -0.7 12,769 - GFF2220 0.70 -1.0 12,804 - GFF2220 0.73 +0.6 12,804 - GFF2220 0.73 -1.2 12,826 + GFF2220 0.74 -0.1 12,869 + GFF2220 0.77 -0.6 12,869 + GFF2220 0.77 -0.9 12,870 - GFF2220 0.77 +0.6 12,930 + GFF2220 0.81 -1.1 12,950 + GFF2220 0.83 -0.6 12,950 + GFF2220 0.83 -0.1 12,983 + GFF2220 0.85 -0.8 12,984 - GFF2220 0.85 -1.7 13,101 - -0.4 13,154 + -1.4 13,154 + -1.1 13,154 + -1.4 13,154 + -1.1 13,155 - -1.1 13,155 - -0.5 13,155 - -1.2 13,191 - -0.3 13,191 - +1.3 13,191 - +1.1 13,298 - -1.2 13,298 - -0.7 13,301 + -0.6 13,403 - GFF2221 0.19 -0.2 13,516 + GFF2221 0.30 -1.6 13,516 + GFF2221 0.30 +0.0 13,516 + GFF2221 0.30 -0.8 13,516 + GFF2221 0.30 -1.0 13,517 - GFF2221 0.30 +1.7 13,526 + GFF2221 0.31 -0.8 13,579 - GFF2221 0.36 +0.5 13,649 - GFF2221 0.43 -0.5 13,652 + GFF2221 0.43 -1.5 13,652 + GFF2221 0.43 -1.7 13,652 + GFF2221 0.43 +0.3 13,653 - GFF2221 0.43 -0.6 13,715 + GFF2221 0.49 +0.9 13,716 - GFF2221 0.49 -0.8 13,750 - GFF2221 0.52 -1.1 13,787 + GFF2221 0.56 -1.0 13,937 + GFF2221 0.70 -1.3 13,938 - GFF2221 0.70 -1.7 13,939 + GFF2221 0.70 -2.4 13,939 + GFF2221 0.70 -1.2 14,300 - +0.4 14,453 + -0.3 14,453 + -1.0 14,454 - -0.6 14,454 - -0.1 14,454 - -0.6 14,454 - +0.2 14,454 - -0.1 14,533 + +0.0 14,567 + -1.1 14,568 - -0.9 14,605 + +1.3 14,605 + -2.5 15,041 - GFF2222 0.37 -1.7 15,044 - GFF2222 0.37 -0.2 15,044 - GFF2222 0.37 -0.8 15,212 - GFF2222 0.49 -0.6 15,212 - GFF2222 0.49 -0.8
Or see this region's nucleotide sequence