Experiment: LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2214 and GFF2215 are separated by 242 nucleotides GFF2215 and GFF2216 are separated by 58 nucleotides GFF2216 and GFF2217 are separated by 334 nucleotides
GFF2214 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 3,759 to 4,622
GFF2214
GFF2215 - hypothetical protein, at 4,865 to 4,996
GFF2215
GFF2216 - DNA polymerase I (EC 2.7.7.7), at 5,055 to 7,841
GFF2216
GFF2217 - GTP-binding protein EngB, at 8,176 to 8,808
GFF2217
Position (kb)
5
6
7
8 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4.229 kb on - strand, within GFF2214 at 4.260 kb on - strand, within GFF2214 at 4.264 kb on - strand, within GFF2214 at 4.326 kb on - strand, within GFF2214 at 4.391 kb on + strand, within GFF2214 at 4.392 kb on - strand, within GFF2214 at 4.392 kb on - strand, within GFF2214 at 4.401 kb on + strand, within GFF2214 at 4.440 kb on + strand, within GFF2214 at 4.441 kb on - strand, within GFF2214 at 4.501 kb on + strand, within GFF2214 at 4.501 kb on + strand, within GFF2214 at 4.502 kb on - strand, within GFF2214 at 4.502 kb on - strand, within GFF2214 at 4.502 kb on - strand, within GFF2214 at 4.542 kb on - strand at 4.670 kb on + strand at 4.744 kb on + strand at 4.793 kb on + strand at 5.009 kb on + strand at 5.010 kb on - strand at 6.140 kb on + strand, within GFF2216 at 6.322 kb on - strand, within GFF2216 at 6.378 kb on - strand, within GFF2216 at 6.393 kb on + strand, within GFF2216 at 6.464 kb on + strand, within GFF2216 at 6.972 kb on + strand, within GFF2216 at 6.972 kb on + strand, within GFF2216 at 7.060 kb on + strand, within GFF2216 at 7.150 kb on - strand, within GFF2216 at 7.170 kb on + strand, within GFF2216 at 7.171 kb on - strand, within GFF2216 at 7.190 kb on + strand, within GFF2216 at 7.193 kb on + strand, within GFF2216 at 7.193 kb on + strand, within GFF2216 at 7.295 kb on + strand, within GFF2216 at 7.397 kb on + strand, within GFF2216 at 7.414 kb on - strand, within GFF2216 at 7.438 kb on - strand, within GFF2216 at 7.456 kb on - strand, within GFF2216 at 7.456 kb on - strand, within GFF2216 at 7.494 kb on + strand, within GFF2216 at 7.495 kb on - strand, within GFF2216 at 7.524 kb on + strand, within GFF2216 at 7.524 kb on + strand, within GFF2216 at 7.686 kb on - strand at 7.701 kb on + strand at 7.874 kb on + strand at 7.875 kb on - strand at 8.004 kb on + strand at 8.014 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.01875 MOI remove 4,229 - GFF2214 0.54 -0.1 4,260 - GFF2214 0.58 +0.5 4,264 - GFF2214 0.58 +0.6 4,326 - GFF2214 0.66 +0.3 4,391 + GFF2214 0.73 +0.2 4,392 - GFF2214 0.73 +1.9 4,392 - GFF2214 0.73 -0.0 4,401 + GFF2214 0.74 +0.0 4,440 + GFF2214 0.79 -0.4 4,441 - GFF2214 0.79 +0.6 4,501 + GFF2214 0.86 +0.1 4,501 + GFF2214 0.86 -0.3 4,502 - GFF2214 0.86 -0.3 4,502 - GFF2214 0.86 +0.1 4,502 - GFF2214 0.86 -0.6 4,542 - +0.1 4,670 + -0.5 4,744 + -0.1 4,793 + +0.9 5,009 + +0.2 5,010 - +0.2 6,140 + GFF2216 0.39 -1.3 6,322 - GFF2216 0.45 -2.5 6,378 - GFF2216 0.47 -2.5 6,393 + GFF2216 0.48 -1.3 6,464 + GFF2216 0.51 -5.6 6,972 + GFF2216 0.69 -0.9 6,972 + GFF2216 0.69 -4.4 7,060 + GFF2216 0.72 -4.4 7,150 - GFF2216 0.75 -2.6 7,170 + GFF2216 0.76 -3.6 7,171 - GFF2216 0.76 -0.3 7,190 + GFF2216 0.77 -3.2 7,193 + GFF2216 0.77 -4.5 7,193 + GFF2216 0.77 -3.1 7,295 + GFF2216 0.80 -2.1 7,397 + GFF2216 0.84 -1.7 7,414 - GFF2216 0.85 -2.3 7,438 - GFF2216 0.86 -3.4 7,456 - GFF2216 0.86 -3.8 7,456 - GFF2216 0.86 -4.1 7,494 + GFF2216 0.88 -1.2 7,495 - GFF2216 0.88 -1.5 7,524 + GFF2216 0.89 -1.5 7,524 + GFF2216 0.89 -2.1 7,686 - -1.1 7,701 + -3.6 7,874 + -0.4 7,875 - -0.6 8,004 + +0.9 8,014 + +0.5
Or see this region's nucleotide sequence